Method and system for determining height of solution accommodated in container

Patent metadata

Field	Value
Publication number	US-2024318997-A1
Application number	US-202218578812-A
Country	US
Kind code	A1
Filing date	Jul 11, 2022
Priority date	Jul 12, 2021
Publication date	Sep 26, 2024
Grant date	—

Abstract

The present disclosure provides a method and a system for determining the height of a solution in a container in order to amplify and detect a target nucleic acid, the method comprising the steps of: using an illuminator to emit infrared light at the container, which is a colored container; using a detector comprising a photosensor, so as to sense the infrared light passing through the container and refracted, the detector being arranged in the direction of sensing the refracted infrared light; using the sensed refracted infrared light so as to acquire an image; and analyzing the acquired image so as to determine the height of the solution in the container.

First claim

What is claimed is:

1 . A method for determining the height of a solution in a container for amplifying and detecting a target nucleic acid, the method comprising:
emitting infrared light to the container using an illuminator, wherein the container is a colored container; sensing the infrared light passing through and refracted by the container using a detector including an optical sensor, wherein the detector is disposed in an orientation such that the detector senses the refracted infrared light; obtaining an image using the refracted infrared light sensed; and determining the height of the solution in the container by analyzing the image obtained.

2 . The method of claim 1 , wherein the infrared light that is emitted to the container includes infrared light wavelengths selected from a wavelength range of 730 nm to 980 nm.

3 . The method of claim 1 , wherein the analyzing of the obtained image includes:
identifying a horizontal edge from the obtained image; and determining the height of the solution on the basis of the identified horizontal edge.

4 . The method of claim 1 , wherein the solution additionally includes a solute.

5 . The method of claim 4 , wherein the solute is a dye.

6 . The method of claim 5 , wherein the dye is a fluorescent, luminescent, and chromogenic dye.

7 . The method of claim 4 , wherein the solute is an oligonucleotide.

8 . The method of claim 4 , wherein the solute is an oligonucleotide bonded with a dye.

9 . The method of claim 1 , wherein the detector is a monochrome detector.

10 . The method of claim 1 , wherein the container has higher transmittance for the infrared light than visible light.

11 . A system for determining the height of a solution in a container for amplifying and detecting a target nucleic acid, the system comprising:
an illuminator for emitting infrared light to the container; a detector for obtaining an image by sensing the infrared light passing through and refracted by the container using the illuminator, wherein the detector is disposed in an orientation such that the detector senses the refracted infrared light; and a controller for determining the height of the solution in the container by analyzing the image obtained by the detector.

12 . The system of claim 11 , wherein the controller identifies a horizontal edge from the obtained image and determines the height of the solution on the basis of the identified horizontal edge.

13 . The system of claim 11 , wherein the detector is a monochrome detector.

Assignees

• Seegene Inc

Inventors

• Han Yoo Jun
• Cho Deog Pill
• Jeong Won Seok
• Lim Hyun Jin

Classifications

• G01F23/292 Primary

  Light {, e.g. infrared or ultraviolet} · CPC title

• G06T2207/30204

  Marker · CPC title

• G06T2207/10064

  Fluorescence image · CPC title

• G06T2207/10048

  Infrared image · CPC title

• G06T7/60

  Analysis of geometric attributes · CPC title