Optical-fiber raman photometer, construction method therefor and application thereof

Patent metadata

Field	Value
Publication number	US-2024310285-A1
Application number	US-202218681426-A
Country	US
Kind code	A1
Filing date	Aug 4, 2022
Priority date	Aug 6, 2021
Publication date	Sep 19, 2024
Grant date	—

Abstract

An optical-fiber Raman photometer. The optical-fiber Raman photometer comprises: a light source portion; a scanning system, wherein the core of the scanning system is based on a laser confocal unit, the scanning system comprises a first filter, a second filter, a first reflector, a second reflector, a scanner and an objective lens, and an excitation light is generated from a fiber laser, then passes through the first filter, the first reflector, the second filter and the second reflector in sequence and enters the scanner, and is then focused by means of the objective lens; a detection system, wherein the detection system performs signal transmission and collection based on a multi-mode optical fiber, an end of the optical fiber is tapered, an incident light enters the optical fiber after being focused by the objective lens, the excitation light is emitted from the tapered end of the optical fiber to excite a probe to generate Raman signals, and the Raman signals are then collected by means of the same optical fiber; and a signal collection system, wherein the signal collection system partially overlaps with the scanning system, and when the Raman signals collected by the optical fiber return to the scanner, the Raman signals return to a Raman spectrometer by means of the second reflector and the second filter, and the Raman spectrometer reads out the Raman signals.

First claim

1 . An optical-fiber Raman photometer, wherein, comprises:
a light source portion, said light source portion is a fiber laser; a scanning system, the core of said scanning system is based on the laser confocal unit, including a first filter, a second filter, a first reflector, a second reflector, a scanner, and an objective lens, when an excitation light is generated from the fiber laser, it passes through the first filter, the first reflector, the second filter and the second reflector in sequence and enters the scanner, and is then focused by means of the objective lens; a detection system, wherein said detection system performs signal transmission and collection based on a multi-mode optical fiber, an end of the optical fiber is tapered, an incident light enters the optical fiber after being focused by the objective lens, an excitation light is emitted from the tapered end of the optical fiber to excite probes to generate Raman signals, and the Raman signals are then collected by means of the same optical fiber; a signal collection system, wherein said signal collection system partially overlaps with the scanning system, and when the Raman signals collected by the optical fiber return to the scanner, the Raman signals return to the Raman spectrometer by means of the second reflector and the second filter, and the Raman spectrometer reads out the Raman signals.

2 . The optical-fiber Raman photometer of claim 1 , wherein, in said light source portion, said excitation center wavelength of the laser is 785 nm±0.5 nm; the output power is adjustable from 0-500 mW; the line width is less than 0.1 nm; the output interface is SMA905 or FC/PC; the working voltage is 220V.

3 . The optical-fiber Raman photometer of claim 1 , wherein, in said scanning system, said first filter is a bandpass filter, which allows the light of 785 nm to pass through; said second filter is a notch filter, for filtering 785 nm incident light; said first reflector and said second reflector are total reflectors, said scanner is a laser confocal scanning unit, and the magnification of said objective lens is 10×, NA 0.25.

4 . The optical-fiber Raman photometer of claim 1 , wherein, in said detection system, said optical fiber is a multi-mode optical fiber, the core is 200 microns, the cladding is 25 microns, and the numerical aperture (NA) is 0.22, the transmission range is 400-1100 nm, and said taper length at the end of the optical fiber is 480 microns.

5 . A method for constructing the optical-fiber Raman photometer of claim 1 , comprising the following steps:
Step 1: assemble the confocal scanning unit, specifically including the following sub-steps: Step 1-1: install the first filter, the first reflector, the second filter, and the second reflector in sequence; Step 1-2: install the objective lens; Step 2: build the scanning system, specifically including the following sub-steps: Step 2-1: connect the fiber laser to the confocal scanning unit through the optical fiber; Step 2-2: debug the optical path, test that the excitation light can pass through the confocal scanning unit and be transmitted out by the objective lens; Step 3: build the detection system, specifically including the following sub-steps: Step 3-1: taper the optical fiber to obtain a tapered optical fiber to improve the efficiency of optical fiber signal collection; Step 3-2: assemble the tapered optical fiber to the end of the scanning system, and debug the excitation light coupling into the optical fiber; Step 4: build the signal collection system, specifically including the following sub-steps: Step 4-1: connect the Raman spectrometer to the confocal scanning unit through the optical fiber; Step 4-2: test the Raman signal collection.

6 . Application of the optical-fiber Raman photometer constructed by the method of claim 5 in collecting the Raman signals in vivo and/or in vitro.

7 . Application of the optical-fiber Raman photometer constructed by the method of claim 5 for in vitro Raman molecular signal collection under the excitation of the excitation light; wherein,
said Raman molecules include rhodamine B, copper titanocyanine, and cyanine dye 5;
said excitation light includes 633 nm, 785 nm;
said Raman molecule concentration is 0.1-5 mM;
the scanning range is 100-3200 cm −1 .

8 . Application of the optical-fiber Raman photometer constructed by the method of claim 5 in collecting Raman molecular signals in different brain regions under the excitation of the excitation light; wherein,
said different brain regions are cortex, hippocampus, striatum, and thalamus;
said excitation light includes 633 nm, 785 nm;
said Raman molecules include rhodamine B, copper titanocyanine, cyanine dye 5;
the scanning range is 100-3200 cm −1 .

9 . A method for constructing the optical-fiber Raman photometer of claim 2 , comprising the following steps:
Step 1: assemble the confocal scanning unit, specifically including the following sub-steps: Step 1-1: install the first filter, the first reflector, the second filter, and the second reflector in sequence; Step 1-2: install the objective lens; Step 2: build the scanning system, specifically including the following sub-steps: Step 2-1: connect the fiber laser to the confocal scanning unit through the optical fiber; Step 2-2: debug the optical path, test that the excitation light can pass through the confocal scanning unit and be transmitted out by the objective lens; Step 3: build the detection system, specifically including the following sub-steps: Step 3-1: taper the optical fiber to obtain a tapered optical fiber to improve the efficiency of optical fiber signal collection; Step 3-2: assemble the tapered optical fiber to the end of the scanning system, and debug the excitation light coupling into the optical fiber; Step 4: build the signal collection system, specifically including the following sub-steps: Step 4-1: connect the Raman spectrometer to the confocal scanning unit through the optical fiber; Step 4-2: test the Raman signal collection.

10 . A method for constructing the optical-fiber Raman photometer of claim 3 , comprising the following steps:
Step 1: assemble the confocal scanning unit, specifically including the following sub-steps: Step 1-1: install the first filter, the first reflector, the second filter, and the second reflector in sequence; Step 1-2: install the objective lens; Step 2: build the scanning system, specifically including the following sub-steps: Step 2-1: connect the fiber laser to the confocal scanning unit through the optical fiber; Step 2-2: debug the optical path, test that the excitation light can pass through the confocal scanning unit and be transmitted out by the objective lens; Step 3: build the detection system, specifically including the following sub-steps: Step 3-1: taper the optical fiber to obtain a tapered optical fiber to improve the efficiency of optical fiber signal collection; Step 3-2: assemble the tapered optical fiber to the end of the scanning system, and debug the excitation light coupling into the optical fiber; Step 4: build the signal collection system, specifically including the following sub-steps: Step 4-1: connect the Raman spectrometer to the confocal scanning unit through the optical fiber; Step 4-2: test the Raman signal collection.

11 . A method for constructing the optical-fiber Raman photometer of claim 4 , comprising the following steps:
Step 1: assemble the confocal scanning unit, specifically including the following sub-steps: Step 1-1: install the first filter, the first reflector, the second filter, and the second reflector in sequence; Step 1-2: install the objective lens;

Assignees

• Univ East China Normal

Inventors

• Tian Yang
• Liu Zhichao

Classifications

• G01J3/10

  Arrangements of light sources specially adapted for spectrometry or colorimetry · CPC title

• G01J3/0227

  using notch filters · CPC title

• G01J3/0208

  using focussing or collimating elements, e.g. lenses or mirrors; performing aberration correction · CPC title

• G01N2021/6484

  Optical fibres · CPC title

• A61B5/4064

  Evaluating the brain (for intracranial pressure A61B5/031; for cerebral blood gases A61B5/14553; using EEG A61B5/369) · CPC title