Antibacterial Composition

Patent metadata

Field	Value
Publication number	US-2024284904-A1
Application number	US-202218563694-A
Country	US
Kind code	A1
Filing date	Sep 28, 2022
Priority date	Oct 21, 2021
Publication date	Aug 29, 2024
Grant date	—

Abstract

An antibacterial composition including a compound represented by the disclosed formula 1 and having an antibacterial activity of 80% or more as measured by the disclosed method 1, against at least one strain of gram-positive bacteria, gram-negative bacteria, and fungus. Since the antibacterial composition includes a hydrophilic functional group and a hydrophobic functional group at the same time, it is advantageous for imparting antibacterial properties.

First claim

1 . An antibacterial composition including one or more compounds represented by formula 1 below and having an antibacterial activity of 80% or more, as measured by method 1 below, against at least one strain of gram-positive bacteria, gram-negative bacteria, and fungus:

wherein,
L1 and L2 are the same as or different from each other, and are each independently a direct bonds a substituted or unsubstituted alkylene group having 1 to 4 carbon atoms, or a substituted or unsubstituted arylene group having 6 to 30 carbon atoms;
A is hydrogen, or a substituted or unsubstituted alkyl group having 1 to 3 carbon atoms-;
n is an integer of 0 to 4;
R1 and R2 are the same as or different from each other, and each independently a substituted or unsubstituted alkyl group having 1 to 20 carbon atoms, and at least one of R1 and R2 is a substituted or unsubstituted alkyl group having 1 to 10 carbon atoms; and
R3 is an alkyl group having 4 to 20 carbon atoms, wherein when n is 2 or more, As having n of 2 or more are the same as or different from each other; and
[Method 1]
after putting 25 mL of a broth-type medium inoculated with 3,000 CFU/mL of bacteria in a 50 mL conical tube, 0.015 g of the antibacterial composition i added and suspended, and a sufficiently mixed solution is cultured for 16 hours in a shaking water bath maintained at 35° C., and
after diluting the cultured solution to 1/5 using 1×PBS buffer solution, an absorbance is measured using a UV/Vis spectrophotometer, and the measured absorbance is compared with the solution cultured without addition of the antibacterial composition to calculate the antibacterial activity, which is a bacteriostatic reduction rate, by an equation as follows:

Antibacterial
⁢

activity
⁢

(
%
)

=

(

1
-

A
sample

/

A
Reference

)

×
100

A
sample

=

Absorbance
⁢

of
⁢

medium
⁢

solution
⁢

cultured

⁢

with
⁢

addition
⁢

of
⁢

antibacterial
⁢

composition

A
Reference

=

Absorbance
⁢

of
⁢

medium
⁢

solution
⁢

cultured

⁢

without
⁢

addition
⁢

of
⁢

antibacterial
⁢

composition

2 . The antibacterial composition of claim 1 , wherein the antibacterial activity is 90% or more.

3 . The antibacterial composition of claim 1 , wherein the gram-positive bacteria are any one selected from Enterococcus faecalis, Staphylococcus aureus, Streptococcus pneumoniae, Streptococcus pyogene, Enterococcus faecium , and Lactobacillus lactis.

4 . The antibacterial composition of claim 1 , wherein the gram-negative bacteria are any one selected from Proteus mirabilis, Escherichia coli, Salmonella typhi, Pseudomonas aeruginosa, Vibrio cholerae , and Enterobacter cloacae.

5 . The antibacterial composition of claim 1 , wherein at least one of the R1 and R2 is an unsubstituted alkyl group having 1 to 4 carbon atoms.

6 . The antibacterial composition of claim 1 , wherein the R3 is an unsubstituted alkyl group having 4 to 16 carbon atoms.

7 . The antibacterial composition of claim 1 , wherein, when the R1 and R2 are the same as or different from each other and each independently a methyl group or an ethyl group, the R3 is an alkyl group having 4 to 16 carbon atoms.

8 . The antibacterial composition of claim 1 , wherein the L1 is a direct bond.

9 . The antibacterial composition of claim 1 , wherein the L2 is a methylene group.

10 . The antibacterial composition of claim 1 , wherein the compound represented by formula 1 above is any one selected from the group consisting of structures as follows:

Assignees

• Lg Chemical Ltd

Inventors

• Lee Seungmo
• Baek Leehyeon
• Lee Jeong Yun
• Lee Hwanhee
• Choi Doowhan
• Hur Yoon Hyung
• Lee Jiyoung
• Choi Hyungsam

Classifications

• Y02A50/30

  Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change · CPC title

• C08F212/28

  Amines · CPC title

• C09D125/18

  Homopolymers or copolymers of aromatic monomers containing elements other than carbon and hydrogen · CPC title

• C08F12/28

  Amines · CPC title

• A01P3/00

  Fungicides · CPC title