Microfluidic detection system with adjustable flow control

Patent metadata

Field	Value
Publication number	US-2024253033-A1
Application number	US-202318505601-A
Country	US
Kind code	A1
Filing date	Nov 9, 2023
Priority date	Nov 9, 2022
Publication date	Aug 1, 2024
Grant date	—

Abstract

There is provided a detection system for detecting an analyte in a sample. The detection system contains a microfluidic chip which has an inlet adapted to receive the sample, an incubation chamber, a sensing chamber, and an outlet. The detection system has a suction membrane in fluid communication with the outlet of the microfluidic chip, and an actuator for applying or relieving pressure from the suction membrane which modifies the air pressure in the microfluidic chip and drives a flow of the sample in the microfluidic chip. Finally, a detection apparatus is also provided for measuring a signal of the analyte in the sensing chamber.

First claim

What is claimed is:

1 . A detection system for detecting an analyte in a sample, comprising:
a microfluidic chip comprising:
an inlet adapted to receive the sample,
an incubation chamber having an incubation chamber inlet fluidly connected to the inlet downstream thereof, to incubate the analyte in the sample,
a sensing chamber fluidly connected to the incubation chamber, downstream of the filter barrier, and
an outlet fluidly connected to the sensing chamber downstream thereof;
a suction membrane in fluid communication with the outlet of the microfluidic chip; an actuator for applying or relieving pressure from the suction membrane which modifies the air pressure in the microfluidic chip and drives a flow of the sample in the microfluidic chip; and a detection apparatus for measuring a signal of the analyte in the sensing chamber.

2 . The detection system of claim 1 , wherein the detection apparatus is a spectroscopy detection apparatus or an electrical detection apparatus.

3 . The detection system of claim 1 , wherein the microfluidic chip further comprises a filter barrier fluidly connected to the incubation chamber, downstream of the incubation chamber inlet.

4 . The detection system of claim 3 , wherein the detection apparatus is a light detection apparatus and comprises
a light source for providing an epi illumination on the sensing chamber of the microfluidic chip, a condensing lens for condensing light from the sensing chamber of the microfluidic chip, and an image sensor receiving the light condensed by the condensing lens, the image sensor adapted to register the light as an electronic signal and to send said electronic signal to a processing device.

5 . The detection system of claim 4 , wherein the microfluidic chip is part of a microfluidic cartridge comprising
an inlet apparatus connected to the microfluidic chip, and covering the inlet, the incubation chamber and the filter barrier of the microfluidic chip, the inlet apparatus comprising:
a receptacle in fluid communication with the inlet of the microfluidic chip, the receptacle being adapted to receive the sample,
a storage chamber having a rupturable membrane and housing a colorimetric sensor, the storage chamber is in fluid communication with the microfluidic chip upstream of the sensing chamber and the filter barrier of the microfluidic chip,
an outlet apparatus connected to the microfluidic chip and covering the outlet, the outlet apparatus is in fluid communication with the outlet of the microfluidic chip, the outlet apparatus comprising:
the actuator, wherein the actuator is a screw adapted to be released in order to drive the flow of the sample to the incubation chamber,
a second suction membrane adapted to be released in order to flow the sample past the filter barrier and to drive a flow of the colorimetric sensor released from the storage chamber.

6 . The detection system of claim 5 , wherein the outlet apparatus comprises a second crew, and wherein the screw is adapted to be released in order to drive the flow of the sample to the incubation chamber, and the second screw is adapted to be released in order to flow the sample past the filter barrier and to drive the flow of the colorimetric sensor released from the storage chamber.

7 . The detection system of claim 5 , further comprising a closing lid for closing the receptacle of the inlet apparatus.

8 . The detection system of claim 5 , further comprising a second storage chamber in the inlet apparatus, the second storage chamber housing lysis reagents and having a rupturable membrane.

9 . The detection system of claim 8 , further comprising a second piercing actuator in the inlet apparatus to pierce the rupturable membrane of the second storage chamber, and the second piercing actuator is connected to the actuating motor.

10 . The detection system of claim 8 , further comprising a heating actuator in the inlet apparatus comprising a heating element for a lysis of the sample, and the heating actuator is connected to the actuating motor.

11 . The detection system of claim 1 , further comprising an imaging box, wherein the imaging box comprises the suction membrane, the detection apparatus and the actuator, and wherein the suction membrane is positioned below a support which is adapted to releasably bind to the microfluidic chip, and wherein the detection apparatus is a light detection apparatus.

12 . The detection system of claim 1 , wherein the flow of the sample is bidirectional.

13 . The detection system of claim 1 , wherein the detection apparatus is a light detection apparatus and wherein the sensing chamber comprises a plasmonic nanosurface, the plasmonic nanosurface including nanostructures protruding from the plasmonic nanosurface, the nanostructures having a size that is smaller than that of the diffraction limit of light, the nanostructures having a metallic layer that is plasmon-supported on top of a back reflector layer.

14 . The detection system of claim 1 , wherein the detection apparatus is an electrical detection apparatus and wherein the sensing chamber comprises a dimeric DNA aptamer gold nanostructure.

15 . The detection system of claim 1 , wherein the microfluidic chip comprises a plurality of the sensing chamber and multiple parallel channels each leading to one of the sensing chambers.

16 . The detection system of claim 15 , further comprising a motorized platform connected to the detection apparatus for moving the detection apparatus between the plurality of the sensing chamber.

17 . The detection system of claim 1 , wherein the analyte is selected from nucleic acid, microorganism, a cell of a multicellular organism, or a protein.

18 . The detection system of claim 1 , further comprises a heating plate.

19 . The detection system of claim 1 , wherein the actuating motor is connected to a controller and the controller is coupled to the processing device.

20 . The detection system of claim 1 , wherein the processing device is selected from a smart phone, a tablet, or a computer.

Assignees

• The Royal Institution For The Advancement Of Learning/Mcgill Univ

Inventors

• MAHSHID Sara
• Jalali Mahsa
• Abdelwahab Tamer
• Yedire Sripadh Guptha
• Hosseini Seyed Imman Isaac

Classifications

• B01L3/502715 Primary

  characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces · CPC title

• B01L3/50273 Primary

  characterised by the means or forces applied to move the fluids · CPC title

• B01L2300/0663

  Whole sensors · CPC title

• B01L2400/0487

  fluid pressure, pneumatics · CPC title

• B01L2300/1805

  Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks · CPC title