Method for decreasing colorectal cancer cells

Patent metadata

Field	Value
Publication number	US-2024252531-A1
Application number	US-202418599582-A
Country	US
Kind code	A1
Filing date	Mar 8, 2024
Priority date	Jan 19, 2022
Publication date	Aug 1, 2024
Grant date	—

Abstract

A method of making Cu—Ag3PO4 nanoparticles is provided. The method includes forming a mixture of at least one silver salt, at least one phosphate salt, and at least one copper (II) salt. The method further includes dissolving the mixture in water. The method further includes sonicating the mixture. The method further includes precipitating the Cu—Ag3PO4 nanoparticles or “nanoparticles”. The copper is present in the nanoparticles in an amount of 2 to 23 weight percent (wt. %) based on the total weight of the Cu—Ag3PO4. The nanoparticles of the present disclosure find application in treating cervical cancer, and colorectal cancer. The nanoparticles may also be used in photodegrading environmental pollutants.

First claim

1 - 10 . (canceled)

11 . A method of decreasing an amount of, colorectal cancer cells in a cell culture, comprising:
contacting a composition with the colorectal cancer cells and incubating in a CO2 incubator wherein the composition comprises Cu—Ag 3 PO 4 substantially spherical nanoparticles having a mean particle size of 100-1,000 nm, wherein an amount of the Cu—Ag 3 PO 4 nanoparticles is 5.0 to 95 μg per mL of the cell culture, wherein the Cu—Ag 3 PO 4 nanoparticles have an IC50 of 36-46 μg per mL of the cell culture, wherein the colorectal cancer cells are HCT-116 cells, wherein copper is present in the Cu—Ag 3 PO 4 nanoparticles in an amount of 10-15 weight percent (wt. %) based on the total weight of the Cu—Ag 3 PO 4 nanoparticles.

12 - 20 . (canceled)

21 . The method of claim 11 , wherein the contacting is for 48 hours.

22 . The method of claim 21 , wherein the colorectal cancer cells are incubated in the CO 2 incubator for 4 hours.

23 . The method of claim 11 , further comprising:
centrifuging the reaction mixture after the precipitating to separate the Cu—Ag 3 PO 4 nanoparticles from the mixture; washing the Cu—Ag 3 PO 4 nanoparticles with ethanol and water; and drying the washed Cu—Ag 3 PO 4 nanoparticles at a temperature less than 150° C.

24 . The method of claim 11 , wherein the Cu—Ag 3 PO 4 nanoparticles have a BET mean surface area of 4.2-6.1 m 2 /g.

25 . The method of claim 24 , wherein the Cu—Ag 3 PO 4 nanoparticles have a BET mean pore size of 19.5-29.6 nm.

26 . The method of claim 11 , wherein during the contacting the Cu—Ag 3 PO 4 nanoparticles are aggregated.

27 . The method of claim 11 , wherein the composition consists of the Cu—Ag 3 PO 4 nanoparticles.

28 . The method of claim 11 , wherein the Cu—Ag 3 PO 4 nanoparticles have a mean particle size of 200-300 nm.

29 . The method of claim 11 , wherein the contacting is in vitro.

Assignees

• Univ Imam Abdulrahman Bin Faisal

Inventors

• Nawaz Muhammad
• Khan Firdos Alam

Classifications

• B82Y5/00

  Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery · CPC title

• C01P2006/14

  Pore volume · CPC title

• C01P2002/84

  by UV- or VIS- data · CPC title

• C01P2002/85

  by XPS, EDX or EDAX data · CPC title

• C01P2004/03

  obtained by SEM · CPC title