Stimulated Raman Scattering Tomography System And Method

Patent metadata

Field	Value
Publication number	US-2024241057-A1
Application number	US-202218563631-A
Country	US
Kind code	A1
Filing date	May 23, 2022
Priority date	May 24, 2021
Publication date	Jul 18, 2024
Grant date	—

Abstract

A stimulated Raman scattering tomography system includes means for generating a first input light beam, means for generating a second input light beam, an objective, a condenser and a detector. The first input light beam is phase-modulated while the second input light beam is amplitude-modulated. The objective is configured to direct the first and second input light beams onto a sample. The condenser is configured to collect an output light beam from the sample. The is detector configured to detect at least a portion of the output light beam corresponding to the first input light beam. The system further includes means for forming a depth-resolved image of the sample from the detected portion of the output light beam.

First claim

1 . A stimulated Raman scattering tomography system comprising:
a laser light source configured to provide a first output and a second output, wherein the first output is configured to generate a first input light beam, wherein the first input light beam is phase-modulated; and wherein the second output is configured to generate a second input light beam, wherein the second input light beam is amplitude-modulated;
an objective configured to direct the first and second input light beams onto a sample;
a condenser configured to collect an output light beam from the sample;
a detector configured to detect at least a portion of the output light beam corresponding to the first input light beam; and
a lock-in amplifier configured to demodulate the detected portion of the output light beam from the sample for forming a depth-resolved image of the sample from the detected portion of the output light beam.

2 . (canceled)

3 . The system as claimed in claim 1 , wherein the laser light source comprises a broadband femtosecond laser light source.

4 . The system as claimed in claim 1 , wherein the first input light beam comprises a pump beam and the second input light beam comprises a Stokes beam.

5 . The system as claimed in claim 3 , further comprising a spatial light modulator for phase-modulating the pump beam based on a predetermined pattern.

6 . The system as claimed in claim 3 , further comprising an electro-optic modulator for amplitude-modulating the Stokes beam at a predetermined frequency.

7 . The system as claimed claim 3 , wherein the pump beam and Stokes beam comprise collinear Bessel beams.

8 . (canceled)

9 . The system as claimed claim 1 , further comprising a bandpass filter set positioned after the condenser and configured to spectrally isolate the portion of the output light beam corresponding to the first input light beam.

10 . (canceled)

11 . (canceled)

12 . The system as claimed in claim 1 , wherein the lock-in amplifier is configured to demodulate the detected portion of the output light beam based on an inverse fast Fourier transform.

13 . The system as claimed in claim 1 , wherein the output light beam comprises a reflected beam from the sample.

14 . The system as claimed in claim 1 , wherein the output light beam comprises a transmitted beam from the sample.

15 . A stimulated Raman scattering tomography method comprising:
generating a first input light beam, wherein the first input light beam is phase-modulated; generating a second input light beam, wherein the second input light beam is amplitude-modulated; directing the first and second input light beams onto a sample; collecting an output light beam from the sample; detecting at least a portion of the output light beam corresponding to the first input light beam; and forming a depth-resolved image of the sample from the detected portion of the output light beam.

16 . The method as claimed in claim 15 , wherein the first input light beam comprises a pump beam and the second input light beam comprises a Stokes beam.

17 . The method as claimed in claim 16 , wherein the pump beam is phase-modulated based on a predetermined pattern using a spatial light modulator.

18 . The method as claimed in claim 15 , wherein the Stokes beam is amplitude-modulated at a predetermined frequency using an electro-optic modulator.

19 . The method as claimed in claim 15 , wherein generating the pump and Stokes beams comprises forming collinear Bessel beams.

20 . The method as claimed in claim 12 , wherein forming the depth-resolved image of the sample comprises demodulating the detected portion of the output light beam from the sample using a lock-in amplifier.

21 . The method as claimed in claim 20 , wherein demodulating the detected portion of the output light beam comprises applying an inverse fast Fourier transform.

22 . A three-dimensional volumetric imaging method comprising the method as claimed in claim 12 .

23 . (canceled)

24 . The three-dimensional volumetric imaging method as claimed in claim 22 , wherein the sample is label-free.

25 . The three-dimensional volumetric imaging method as claimed in claim 22 , wherein directing the first and second input light beams onto the sample comprises directing the first and second input light beams without scanning a focus of the first or second input light beams across a depth of the sample.

Assignees

• Nat Univ Singapore

Inventors

• Huang Zhiwei
• Gong Li
• Lin Shulang

Classifications

• G02B21/33

  Immersion oils {, or microscope systems or objectives for use with immersion fluids} · CPC title

• G01N2201/0697

  Pulsed lasers · CPC title

• G01N2201/0675

  SLM · CPC title

• G01N2201/06113

  Coherent sources; lasers · CPC title

• G01N2021/655

  Stimulated Raman · CPC title