Flow cells

Patent metadata

Field	Value
Publication number	US-2024207842-A1
Application number	US-202318542511-A
Country	US
Kind code	A1
Filing date	Dec 15, 2023
Priority date	Dec 16, 2022
Publication date	Jun 27, 2024
Grant date	—

Abstract

An example of a flow cell includes a substrate; a plurality of reactive regions spatially separated from one another across the substrate; and a plurality of independently removable coatings respectively positioned over each of the plurality of reactive regions. Each of the plurality of reactive regions includes a polymeric hydrogel layer; and a reactive entity attached to the polymeric hydrogel layer. At least one of the independently removable coatings is a gas-dissolvable coating.

First claim

What is claimed is:

1 . A flow cell, comprising:
a substrate; a plurality of reactive regions spatially separated from one another across the substrate, each of the plurality of reactive regions including:
a polymeric hydrogel layer; and
a reactive entity attached to the polymeric hydrogel layer; and
a plurality of independently removable coatings respectively positioned over each of the plurality of reactive regions; wherein at least one of the plurality of independently removable coatings is a gas-dissolvable coating.

2 . The flow cell as defined in claim 1 , wherein the reactive entity in each of the plurality of reactive regions is a primer set.

3 . The flow cell as defined in claim 2 , wherein the primer set is the same in each of the plurality of reactive regions.

4 . The flow cell as defined in claim 2 , wherein the primer set of at least one of the plurality of reactive regions is different than the primer set of at least one other of the plurality of reactive regions.

5 . The flow cell as defined in claim 1 , wherein:
the substrate includes a plurality of depressions; each of the plurality of reactive regions is positioned within a respective one of the plurality of depressions; and each of the plurality of independently removable coatings covers a respective one of the plurality of reactive regions.

6 . The flow cell as defined in claim 1 , wherein:
the substrate includes a plurality of protrusions; each of the plurality of reactive regions is positioned at a respective one of the plurality of protrusions; and each of the plurality of independently removable coatings covers a respective one of the plurality of protrusions.

7 . The flow cell as defined in claim 1 , wherein the gas-dissolvable coating is selected from the group consisting of an amine-based coating, an amidine-based coating, a guanidine-based coating, an oxygen-responsive copolymer, an oxidation-responsive copolymer, and combinations thereof.

8 . A method, comprising:
selectively removing at least one of a plurality of independently removable coatings respectively positioned over each of a plurality of reactive regions spatially separated from one another across a substrate, thereby exposing at least one of the plurality of reactive regions and a reactive entity at the at least one of the plurality of reactive regions;
wherein each of the plurality of reactive regions includes a polymeric hydrogel layer and a reactive entity attached to the polymeric hydrogel layer; and
wherein at least one of the plurality of independently removable coatings is a gas-dissolvable coating; and
initiating a reaction involving the reactive entity.

9 . The method as defined in claim 8 , wherein selectively removing the at least one of the plurality of independently removable coatings involves exposing the at least one of the plurality of independently removable coatings to a reactive gas and water in the presence of an inert gas, thereby dissolving the at least one of the plurality of independently removable coatings.

10 . The method as defined in claim 8 , wherein:
the substrate includes a plurality of depressions; each of the plurality of reactive regions is positioned within a respective one of the plurality of depressions; and each of the plurality of independently removable coatings covers a respective one of the plurality of reactive regions.

11 . The method as defined in claim 8 , wherein:
the substrate includes a plurality of protrusions; each of the plurality of reactive regions is positioned at a respective one of the plurality of protrusions; and each of the plurality of independently removable coatings covers a respective one of the plurality of protrusions.

12 . The method as defined in claim 8 , wherein the reactive entity in each of the plurality of reactive regions is a primer set.

13 . The method as defined in claim 12 , wherein the primer set is the same in each of the plurality of reactive regions.

14 . The method as defined in claim 12 , wherein the primer set of at least one of the plurality of reactive regions is different than the primer set of at least one other of the plurality of reactive regions.

15 . The method as defined in claim 8 , wherein the gas-dissolvable coating is selected from the group consisting of an amine-based coating, an amidine-based coating, a guanidine-based coating, an oxygen-responsive copolymer, an oxidation-responsive copolymer, and combinations thereof.

16 . A flow cell, comprising:
a substrate; a plurality of reactive regions spatially separated from one another across the substrate, each of the plurality of reactive regions including:
a polymeric hydrogel layer; and
a reactive entity attached to the polymeric hydrogel layer;
a heating mechanism aligned with at least one of the plurality of reactive regions; and a plurality of independently removable coatings respectively positioned over each of the plurality of reactive regions; wherein at least one of the plurality of independently removable coatings is a heat-responsive coating.

17 . The flow cell as defined in claim 16 , wherein the reactive entity in each of the plurality of reactive regions is a primer set.

18 . The flow cell as defined in claim 17 , wherein the primer set is the same in each of the plurality of reactive regions.

19 . The flow cell as defined in claim 17 , wherein the primer set of at least one of the plurality of reactive regions is different than the primer set of at least one other of the plurality of reactive regions.

20 . The flow cell as defined in claim 16 , wherein:
the substrate includes a plurality of depressions; each of the plurality of reactive regions is positioned within a respective one of the plurality of depressions; each of the plurality of independently removable coatings covers a respective one of the plurality of reactive regions.

21 . The flow cell as defined in claim 16 , wherein:
the substrate includes a plurality of protrusions; each of the plurality of reactive regions is positioned at a respective one of the plurality of protrusions; and each of the plurality of independently removable coatings covers a respective one of the plurality of protrusions.

22 . The flow cell as defined in claim 16 , wherein the heat-responsive coating is selected from the group consisting of polylactic acid, poly(lactic-co-glycolic) acid, polycaprolactone, agarose, wax, poly(acrylamide-co-acrylonitrile), poly(N-isopropylacrylamide), cyclodextrins, polyethylene glycol homopolymer, polyethylene glycol graft copolymer, polyethylene block copolymer, and a combination thereof.

23 . A method, comprising:
selectively removing at least one of a plurality of independently removable coatings respectively positioned over each of a plurality of reactive regions spatially separated from one another across a substrate by activating at least one heating mechanism, thereby exposing at least one of the plurality of reactive regions and a reactive entity at the at least one of the plurality of reactive regions;
wherein each of the plurality of reactive regions includes a polymeric hydrogel layer and a reactive entity attached to the polymeric hydrogel layer;
wherein at least one of the plurality of independently removable coatings is a heat-responsive coating; and
wherein at least one of the plurality of reactive regions aligns with the at least one heating mechanism; and
initiating a

Assignees

• Illumina Inc

Inventors

• Artioli Gianluca Andrea
• Basuki Johan Sebastian
• Boyanov Boyan
• Canzi Gabriele
• Hong Sahngki
• Nguyen Nam
• Ricoult Sebastien Georg Gabriel
• Szemjonov Alexandra
• Von Hatten Xavier

Classifications

• B01J2219/00722

  Nucleotides · CPC title

• B01J2219/00644

  the porous medium being present in discrete locations, e.g. gel pads · CPC title

• B01J2219/00637

  by coating it with another layer · CPC title

• B01J2219/00621

  by physical means, e.g. trenches, raised areas · CPC title

• B01J2219/00608

  DNA chips · CPC title