Mass spectrometry analysis of microorganisms in samples

Patent metadata

Field	Value
Publication number	US-2024194469-A1
Application number	US-202318519342-A
Country	US
Kind code	A1
Filing date	Nov 27, 2023
Priority date	Jun 25, 2013
Publication date	Jun 13, 2024
Grant date	—

Abstract

The invention generally relates to systems and methods for mass spectrometry analysis of microorganisms in samples.

First claim

1 - 18 . (canceled)

19 . A method for capturing a target analyte from a sample, the method comprising:
providing a product comprising a capture module configured to capture a target analyte from a sample, wherein the capture module comprises a porous substrate that is held between a top and bottom portion of a cartridge and an electrode, the top and bottom portion sandwiching the porous substrate and each having an opening therein such that a portion of the porous substrate is exposed when the porous substrate in held in the cartridge and the electrode contacting the porous substrate when the porous substrate in held in the cartridge; and moving air or liquid through the exposed portion of the porous substrate such that any target analyte in the air or liquid is retained and captured by the porous substrate.

20 . The method according to claim 19 , wherein the air or liquid comprises a plurality of unknown microorganisms.

21 . The method according to claim 19 , wherein the porous substrate is discrete from a flow of solvent.

22 . The method according to claim 19 , wherein the porous substrate tapers to at least one distal tip.

23 . The method according to claim 19 , further comprising a solvent applied to the porous substrate, wherein optionally, the solvent comprises an internal standard.

24 . The method according to claim 19 , wherein the target analyte is a microorganism is selected from the group consisting of a bacterium, a virus, a protozoan, and a fungus.

25 . The method of claim 19 , wherein the electrode is associated with the top portion of the cartridge.

Assignees

• Purdue Research Foundation

Inventors

• Cooks Robert Graham
• Hamid Ahmed Mohamed
• Jarmusch Alan Keith
• Ouyang Zheng

Classifications

• H01J49/0445 Primary

  with means for introducing as a spray, a jet or an aerosol (electrospray ion sources H01J49/165) · CPC title

• C12Q1/24

  Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms · CPC title

• C12N13/00

  Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves · CPC title

• C12Q1/04 Primary

  Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor {(C12Q1/6897 takes precedence)} · CPC title

• G01N2560/00

  Chemical aspects of mass spectrometric analysis of biological material · CPC title