Device and Method for Separating Blood Plasma from Whole Blood

Patent metadata

Field	Value
Publication number	US-2024116051-A1
Application number	US-202218263819-A
Country	US
Kind code	A1
Filing date	Feb 8, 2022
Priority date	Feb 8, 2021
Publication date	Apr 11, 2024
Grant date	—

Abstract

A microfluidic device includes at least one separation chamber having an inlet and an outlet. The inlet opens into the separation chamber at a lower height than the outlet. In order to separate blood plasma from whole blood, whole blood is introduced into the separation chamber of the microfluidic device, blood cells are sedimented from the whole blood, and blood plasma separated in the process is sublayered using a transport medium which has a higher density than the blood plasma.

First claim

1 . A microfluidic device comprising at least one separation chamber with an inlet and an outlet, wherein the inlet is configured to open into the separation chamber at a lower height than the outlet.

2 . The microfluidic device according to claim 1 , wherein at least one sensor is arranged in the separation chamber.

3 . The microfluidic device according to claim 1 , further comprising a filter arranged at and/or in the outlet.

4 . The microfluidic device according to claim 1 , wherein the outlet is disposed in a side wall of the separation chamber and a sample inlet is disposed in a top of the separation chamber.

5 . The microfluidic device according to claim 4 , wherein the outlet is configured to open into another chamber having a common wall with the separation chamber.

6 . The microfluidic device according to claim 5 , wherein the separation chamber and the further chamber are inclined together towards the further chamber.

7 . A method for separating blood plasma from whole blood, comprising:
introducing the whole blood into the separation chamber of a microfluidic device according to claim 1 , sedimenting blood cells from the whole blood, and at least partially discharging blood plasma separated by sedimentation via the outlet.

8 . The method according to claim 7 , wherein the step of at least partially discharging blood plasma is performed by sublayering with a transport medium.

9 . The method according to claim 8 , wherein the step of sublayering is continued until a phase boundary between the blood plasma and a residue of the whole blood has reached a predeterminable height in the separation chamber.

10 . The method according to 8 , wherein the step of sublayering is continued until a predeterminable amount of the transport medium has been introduced into the separation chamber.

11 . The method according to claim 7 , wherein a remainder of the whole blood is transported out of the separation chamber by way of a transport medium.

12 . The method according to claim 7 , wherein the step of at least partially discharging blood plasma is performed by sublayering with a transport medium which has a higher density than the blood plasma.

Assignees

• Bosch Gmbh Robert

Inventors

• Bott Hannah

Classifications

• B01L3/502753 Primary

  characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation · CPC title

• B01L2200/0652

  Sorting or classification of particles or molecules · CPC title

• B01L2300/0874

  Three dimensional network · CPC title

• B01L2300/0681

  Filter · CPC title

• B01L2400/0481

  squeezing of channels or chambers · CPC title