Cellulosimicrobium cellulans strain and use thereof in straw field composting

Patent metadata

Field	Value
Publication number	US-2024059624-A1
Application number	US-202318350084-A
Country	US
Kind code	A1
Filing date	Jul 11, 2023
Priority date	Aug 16, 2022
Publication date	Feb 22, 2024
Grant date	—

Abstract

The present disclosure provides a Cellulosimicrobium cellulans strain and use thereof in straw field composting, belonging to the technical field of microorganisms. In the present disclosure, the Cellulosimicrobium cellulans strain is named Cellulosimicrobium cellulans MC29-GFP, and is deposited in the China General Microbiological Culture Collection Center (CGMCC), with a deposit number of CGMCC No. 25013. The Cellulosimicrobium cellulans MC29-GFP can promote the compost decomposition of straw in field. The experimental results show that the Cellulosimicrobium cellulans MC29-GFP significantly increases activities of cellulase and peroxidase in the straw during the decomposition compared with Bacillus amyloliquefaciens SQR9, thereby achieving rapid decomposition.

First claim

What is claimed is:

1 . A Cellulosimicrobium cellulans strain, wherein the Cellulosimicrobium cellulans strain is named Cellulosimicrobium cellulans MC29-GFP, and is deposited in the China General Microbiological Culture Collection Center (CGMCC), with a deposit number of CGMCC No. 25013.

2 . A culture method of the Cellulosimicrobium cellulans strain according to claim 1 , comprising the following steps: inoculating the Cellulosimicrobium cellulans strain into an LB medium to prepare a seed liquid, inoculating the seed liquid into a liquid LB medium, and conducting culture to obtain a bacterial solution; wherein
the bacterial solution has the Cellulosimicrobium cellulans strain at a concentration of 1.0×10 9 cfu/mL to 2.5×10 9 cfu/mL.

3 . A method for quickly decomposing straw in field, comprising the following steps: adjusting the straw to a carbon/nitrogen (C/N) ratio of (25-35):1, mixing the straw with a bacterial solution prepared by the culture method according to claim 2 , and conducting composting.

4 . The method according to claim 3 , wherein the C/N ratio of the straw is adjusted by adding a nitrogen source; and the nitrogen source is a urea ammonium nitrate (UAN) solution.

5 . The method according to claim 3 , wherein the bacterial solution is inoculated at 0.08% to 0.12% of whole compost.

6 . The method according to claim 3 , wherein the composting is conducted for 57 d to 63 d.

7 . A straw organic fertilizer prepared by the method according to claim 3 .

Assignees

• Univ Anhui Agricultural

Inventors

• Wei Zhengyu
• Ma Chao
• Yin Zhong
• Zhang Ziyun
• Hu Zhanqin
• Wang Xin
• Song Shengli
• Jiang Lanshan
• Hu Hongxiang
• Zhang Zhen
• Ye Xinxin

Classifications

• C12N1/205 Primary

  Bacterial isolates · CPC title

• C05F17/20 Primary

  using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment (use of invertebrates C05F17/05) · CPC title

• C05F11/08

  Organic fertilisers containing added bacterial cultures, mycelia or the like · CPC title

• C05G3/80

  Soil conditioners · CPC title

• C12N1/20

  Bacteria; Culture media therefor · CPC title