Cartridge and immunochromatographic assay apparatus

Patent metadata

Field	Value
Publication number	US-2024009663-A1
Application number	US-202318471498-A
Country	US
Kind code	A1
Filing date	Sep 21, 2023
Priority date	Mar 24, 2021
Publication date	Jan 11, 2024
Grant date	—

Abstract

The cartridge includes a carrier having a spotting region and an assay region, a first reagent holding part that holds a first reagent and that starts a supply of the first reagent to the carrier by directly or indirectly receiving an external force, a second reagent holding part that starts a supply of the second reagent to the carrier by directly or indirectly receiving an external force exerted by an internal mechanism provided in the immunochromatographic assay apparatus, and a suppression structure that suppresses transmission of an external force exerted by a user directly or indirectly to the second reagent holding part or application of an external force by the user to the second reagent holding part, while allowing the external force exerted by the internal mechanism to be transmitted to the second reagent holding part.

First claim

1 . A cartridge that is attachably and detachably loaded into an immunochromatographic assay apparatus, the cartridge comprising:
a carrier having a spotting region on which a sample is spotted and an assay region in which a color development state changes depending on whether the sample is positive or negative; a first reagent holding part that holds a first reagent and that starts a supply of the first reagent to the carrier by directly or indirectly receiving an external force; a second reagent holding part that holds a second reagent that is supplied to the carrier after the first reagent is supplied to the carrier and that starts a supply of the second reagent to the carrier by directly or indirectly receiving an external force exerted by an internal mechanism provided in the immunochromatographic assay apparatus; and
a suppression structure that suppresses transmission of an external force exerted by a user directly or indirectly to the second reagent holding part or application of an external force by the user to the second reagent holding part, while allowing the external force exerted by the internal mechanism to be transmitted to the second reagent holding part.

2 . The cartridge according to claim 1 ,
wherein the suppression structure has a cover member that covers the second reagent holding part and has an opening for exerting the external force on the second reagent holding part, and the opening has a form in which a member of the internal mechanism is allowed to be inserted, but a finger of the user is not allowed to be inserted.

3 . The cartridge according to claim 2 ,
wherein an opening width of the opening is 5 mm or less.

4 . The cartridge according to claim 1 ,
wherein the suppression structure has a pressed part that is deformed or displaced by receiving a pressing force as the external force and that transmits an operating force that is a force of magnitude necessary for starting the supply of the second reagent to the second reagent holding part by the deformation or the displacement.

5 . The cartridge according to claim 4 ,
wherein the pressed part transmits the operating force to the second reagent holding part in a case where the pressing force is 50 N or more but does not transmit the operating force in a case where the pressing force is less than 50 N.

6 . The cartridge according to claim 1 , further comprising:
a cover member having a pressed part that is deformed or displaced by receiving a pressing force as the external force and that transmits an operating force that is a force of magnitude necessary for starting the supply of the second reagent to the second reagent holding part by the deformation or the displacement, or an opening for exerting the external force on the second reagent holding part, wherein the suppression structure has the pressed part or a coating member that covers the opening.

7 . The cartridge according to claim 1 ,
wherein at least the second reagent among the first reagent and the second reagent is an amplifying liquid that amplifies color development in the assay region.

8 . The cartridge according to claim 1 ,
wherein the first reagent and the second reagent are amplifying liquids that amplify color development in the assay region by reacting with both of the first reagent and the second reagent.

9 . The cartridge according to claim 1 ,
wherein the carrier further includes a color development region in which a color development state changes by a reaction with the first reagent, and in a case where a direction toward the assay region with respect to the spotting region is defined as a downstream side of the carrier, the color development region is disposed on a downstream side of the assay region and the first reagent holding part is provided on an upstream side of the spotting region.

10 . The cartridge according to claim 1 ,
wherein in a case where a direction toward the assay region with respect to the spotting region is defined as a downstream side of the carrier, the carrier has a control region that is provided on a downstream side of the assay region and that shows a development of the sample supplied from the spotting region to the carrier in the assay region by a change in color development state.

11 . An immunochromatographic assay apparatus comprising:
a loading part in which the cartridge according to claim 1 is attachably and detachably loaded; and the internal mechanism.

12 . The immunochromatographic assay apparatus according to claim 11 ,
wherein the suppression structure has a cover member that covers the second reagent holding part and has an opening for exerting the external force on the second reagent holding part, and the opening has a form in which a member of the internal mechanism is allowed to be inserted, but a finger of the user is not allowed to be inserted, and wherein the internal mechanism includes an insertion member capable of being inserted into the opening.

13 . The immunochromatographic assay apparatus according to claim 11 ,
wherein the suppression structure has a pressed part that is deformed or displaced by receiving a pressing force as the external force and that transmits an operating force that is a force of magnitude necessary for starting the supply of the second reagent to the second reagent holding part by the deformation or the displacement, and wherein the internal mechanism is capable of pressing the pressed part with a pressing force of 50 N or more.

Assignees

• Fujifilm Corp

Inventors

• Tanaka Yasutake
• Ishii Hiroyasu

Classifications

• B01L3/5023 Primary

  with a sample being transported to, and subsequently stored in an absorbent for analysis · CPC title

• B01L2400/0481

  squeezing of channels or chambers · CPC title

• B01L2200/16

  Reagents, handling or storing thereof · CPC title

• B01L2300/0825

  Test strips · CPC title

• B01L2400/0683

  mechanically breaking a wall or membrane within a channel or chamber · CPC title