Neuronal cell cultures as compute substrates
US-2024386258-A1 · Nov 21, 2024 · US
Production method for stage 5 neurons
US2023399611A1 · US · A1
| Field | Value |
|---|---|
| Publication number | US-2023399611-A1 |
| Application number | US-202318332209-A |
| Country | US |
| Kind code | A1 |
| Filing date | Jun 9, 2023 |
| Priority date | Jun 14, 2022 |
| Publication date | Dec 14, 2023 |
| Grant date | — |
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Abstract
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A production method for stage 5 neurons with mature dendrites includes culturing stage 3 neurons with immature dendrites in a culture medium containing B-27 Plus.
First claim
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What is claimed is: 1 . A production method for stage 5 neurons with mature dendrites comprising: culturing stage 3 neurons with immature dendrites in a culture medium containing B-27 Plus. 2 . The production method according to claim 1 , wherein the stage 5 neurons have dendritic spines. 3 . The production method according to claim 1 , wherein a postsynaptic structure including drebrin and PSD95 is formed in the stage 5 neurons. 4 . The production method according to claim 1 , wherein the stage 3 neurons express a neuronal marker including MAP2 and neurofilament heavy chain (NF-H1) and are polarized. 5 . The production method according to claim 1 , wherein the stage 3 neurons are obtained by differentiation induction of induced pluripotent stem cells (iPSCs). 6 . The production method according to claim 5 , wherein the stage 3 neurons are induced to differentiate by induction of expression of a transcription factor in the iPSCs. 7 . Stage 5 neurons with mature dendrites that are isolated and cultured in a container. 8 . A screening method for a therapeutic drug for a neurological disease, comprising: culturing stage 3 neurons that are induced to differentiate from neurological disease patient-derived iPSCs in a culture medium containing B-27 Plus in the presence of a test substance to obtain stage 5 neurons; and assessing a phenotype of the stage 5 neurons, wherein a case in which the phenotype of the stage 5 neurons in the presence of the test substance exhibits a phenotype more similar to a wild-type as compared with a phenotype of the stage 5 neurons in the absence of the test substance indicates that the test substance is a candidate of the therapeutic drug for the neurological disease. 9 . A screening method for a therapeutic drug for a neurological disease, comprising: culturing stage 5 neurons that are induced to differentiate from neurological disease patient-derived iPSCs for a predetermined period of time in the presence of a test substance; and assessing a phenotype of the stage 5 neurons, wherein a case in which the phenotype of the stage 5 neurons in the presence of the test substance exhibits a phenotype more similar to a wild-type as compared with a phenotype of the stage 5 neurons in the absence of the test substance indicates that the test substance is a candidate of the therapeutic drug for the neurological disease.
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- C12N5/0619Primary
Neurons · CPC title
from artificially induced pluripotent stem cells · CPC title
Drug screening · CPC title
Vitamins · CPC title
Insulin-transferrin; Insulin-transferrin-selenium · CPC title
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- What does patent US2023399611A1 cover?
- A production method for stage 5 neurons with mature dendrites includes culturing stage 3 neurons with immature dendrites in a culture medium containing B-27 Plus.
- Who is the assignee on this patent?
- Lin Waka, Watanabe Hikaru, Shiomoto Shusaku, and 1 more
- What technology area does this patent fall under?
- Primary CPC classification C12N5/0619. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Thu Dec 14 2023 00:00:00 GMT+0000 (Coordinated Universal Time) (A1). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).