Detection method and detection apparatus

Patent metadata

Field	Value
Publication number	US-2023398552-A1
Application number	US-202318457404-A
Country	US
Kind code	A1
Filing date	Aug 29, 2023
Priority date	Mar 18, 2021
Publication date	Dec 14, 2023
Grant date	—

Abstract

In a detection method, first dielectric particles each capable of being bound to a first target substance and second dielectric particles each capable of being bound to a second target substance are caused to react with a sample that contains a first target substance and a second target substance, the second dielectric particles having a different dielectrophoretic property from the first dielectric particles, a first composite particle to which the first target substance is bound is separated from the other first dielectric particle, and a second composite particle to which the second target substance is bound is separated from the other second dielectric particle by causing dielectrophoresis in the sample after the reaction, and the first target substance contained in the separated first composite particle and the second target substance contained in the second composite particle are each detected.

First claim

What is claimed is:

1 . A detection method comprising:
causing first dielectric particles and second dielectric particles to react with a sample that contains a first target substance and a second target substance, the first dielectric particles being modified with first substances having a property of specifically binding to the first target substance, the second dielectric particles being modified with second substances having a property of specifically binding to the second target substance different from the first target substance and having a different dielectrophoretic property from the first dielectric particles; by causing dielectrophoresis in the sample after the reaction, separating a first composite particle that is a first dielectric particle to which the first target substance is bound among the first dielectric particles from the other first dielectric particle, and separating a second composite particle that is a second dielectric particle to which the second target substance is bound among the second dielectric particles from the other second dielectric particle; and detecting each of the first target substance and the second target substance, the first target substance being contained in the separated first composite particle, the second target substance being contained in the separated second composite particle.

2 . The detection method according to claim 1 ,
wherein when the dielectrophoresis is caused, first dielectrophoresis and second dielectrophoresis are caused in a time-division manner, the first dielectrophoresis being based on an alternating current voltage at a first frequency, the second dielectrophoresis being based on an alternating current voltage at a second frequency different from the first frequency.

3 . The detection method according to claim 1 ,
wherein a particle diameter of a base material portion of the first dielectric particle excluding the first substance is different from a particle diameter of a base material portion of the second dielectric particle excluding the second substance.

4 . The detection method according to claim 1 ,
wherein when the dielectrophoresis is caused, the dielectrophoresis is caused by generating a non-uniform electric field in the sample.

5 . The detection method according to claim 1 ,
wherein each of the first substance and the second substance is an antibody.

6 . The detection method according to claim 1 ,
wherein a spectroscopic property of a base material portion of the first dielectric particle excluding the first substance is different from a spectroscopic property of a base material portion of the second dielectric particle excluding the second substance.

7 . A detection apparatus comprising:
a reaction section that causes first dielectric particles and second dielectric particles to react with a sample that contains a first target substance and a second target substance, the first dielectric particles being modified with first substances having a property of specifically binding to the first target substance, the second dielectric particles being modified with second substances having a property of specifically binding to the second target substance different from the first target substance and having a different dielectrophoretic property from the first dielectric particles; a separator that separates a first composite particle that is a first dielectric particle to which the first target substance is bound among the first dielectric particles from the other first dielectric particle and separates a second composite particle that is a second dielectric particle to which the second target substance is bound among the second dielectric particles from the other second dielectric particle by causing dielectrophoresis in the sample after the reaction; and a detector that detects each of the first target substance and the second target substance, the first target substance being contained in the separated first composite particle, the second target substance being contained in the separated second composite particle.

8 . A detection method comprising:
(a) accommodating a sample that contains first target substances and second target substances, first dielectric particles, and second dielectric particles in a first space, thereby the first space containing a first composite particle, a second composite particle, a third dielectric particle, and a fourth dielectric particle, each of the first dielectric particles contains a first base material and a first substance, which modifies the first base material, each of the second dielectric particles contains a second base material and a second substance, which modifies the second base material, the first dielectric particles contain the third dielectric particle and a fifth dielectric particle, the second dielectric particles contain the fourth dielectric particle and a sixth dielectric particle, the first composite particle contains the fifth dielectric particle and a third target substance, which is contained in the first target substances, and the fifth dielectric particle and the third target substance are bound to each other via the first substance, the second composite particle contains the sixth dielectric particle and a fourth target substance, which is contained in the second target substances, and the sixth dielectric particle and the fourth target substance are bound to each other via the second substance, the third dielectric particle is not bound to any of the first target substances, the third dielectric particle is not bound to any of the second target substances, the fourth dielectric particle is not bound to any of the first target substances, the fourth dielectric particle is not bound to any of the second target substances, the first substance is capable of being directly bound to one of the first target substances, the second substance is capable of being directly bound to one of the second target substances, the first substance is incapable of being directly bound to any of the second target substances, the second substance is incapable of being directly bound to any of the first target substances, and a particle diameter of the first base material is larger than a particle diameter of each second base material; and (b) applying, at different times, voltages having frequencies to a first region included in the first space, thereby the third dielectric particle, the fourth dielectric particle, the first composite particle, and the second composite particle being each detected, the frequencies being different from each other.

Assignees

• Panasonic Ip Man Co Ltd

Inventors

• Arimoto Satoshi
• Kanno Takashi
• HIRAOKA Rui

Classifications

• B03C5/005 Primary

  Dielectrophoresis, i.e. dielectric particles migrating towards the region of highest field strength · CPC title

• G01N15/1434

  Optical arrangements · CPC title

• G01N33/6854 Primary

  Immunoglobulins · CPC title

• B03C5/022

  Non-uniform field separators · CPC title

• G01N27/44721

  by optical means · CPC title