Systems and methods for fluorescence detection with a movable detection module
US-10669576-B2 · Jun 2, 2020 · US
US2023258862A1 · US · A1
| Field | Value |
|---|---|
| Publication number | US-2023258862-A1 |
| Application number | US-202318138700-A |
| Country | US |
| Kind code | A1 |
| Filing date | Apr 24, 2023 |
| Priority date | Dec 16, 2016 |
| Publication date | Aug 17, 2023 |
| Grant date | — |
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System and methods for optical power distribution to a large numbers of sample wells within an integrated device that can analyze single molecules and perform nucleic acid sequencing are described. The integrated device may include a grating coupler configured to receive an optical beam from an optical source and optical splitters configured to divide optical power of the grating coupler to waveguides of the integrated device positioned to couple with the sample wells. Outputs of the grating coupler may vary in one or more dimensions to account for an optical intensity profile of the optical source.
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What is claimed is: 1 . An integrated device comprising: a metal layer disposed on a surface of the integrated device, the metal layer having a discontinuity; and at least one sample well having a top aperture corresponding with the discontinuity of the metal layer, and wherein a surface of the at least one sample well extends beyond the metal layer along a direction that is substantially perpendicular to the surface of the integrated device.
characterised by the input or output waveguides, e.g. tapered waveguide ends, coupled together pairs of output waveguides · CPC title
for batch operation, i.e. multisample apparatus (analytical automats G01N35/00) · CPC title
Individual samples arranged in a regular 2D-array, e.g. multiwell plates · CPC title
using evanescent coupling or surface plasmon coupling for the excitation of fluorescence · CPC title
using reagent-clad optical fibres or optical waveguides (using measurement of total internal reflection or attenuated total reflection G01N21/552; optical fibres or waveguides per se G02B) · CPC title
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