Detection kit, detection apparatus and method of manufacturing detection kit

Patent metadata

Field	Value
Publication number	US-2023251183-A1
Application number	US-202318164240-A
Country	US
Kind code	A1
Filing date	Feb 3, 2023
Priority date	Feb 4, 2022
Publication date	Aug 10, 2023
Grant date	—

Abstract

A detection kit, a detection apparatus and a method for manufacturing a detection kit are provided. The detection kit according to an aspect of the present invention may include a plate-shaped detection member; a first photothermal area which is formed on one side of the detection member and fixes a photothermal material that generates heat exposed to light; and a first sample area which is disposed adjacent to the first photothermal area to receive heat from the first photothermal area when light is irradiated onto the first photothermal area.

First claim

1 . A detection kit, comprising:
a plate-shaped detection member; a first photothermal area which is formed on one side of the detection member and fixes a photothermal material that generates heat exposed to light; and a first sample area which is disposed adjacent to the first photothermal area to receive heat from the first photothermal area when light is irradiated onto the first photothermal area.

2 . The detection kit of claim 1 , wherein the first photothermal area is formed in a hollow tubular shape extending from one side to the other side of the detection member, and
wherein the first sample area extends from one side to the other side of the detection member and is formed inside the first photothermal area.

3 . The detection kit of claim 2 , wherein the first photothermal area is formed in a cylindrical shape.

4 . The detection kit of claim 1 , wherein the detection member is formed of a material comprising cellulose to have hygroscopicity.

5 . The detection kit of claim 1 , wherein the photothermal material is a mixture of carbon black and poly(dimethylsiloxane).

6 . The detection kit of claim 5 , wherein the wavelength of light exposed to the photothermal material is 800 nm to 1,000 nm.

7 . The detection kit of claim 5 , wherein the concentration of carbon black of the photothermal material is 0.5 to 1.5%.

8 . The detection kit of claim 1 , wherein the photothermal material is a mixture of gold nanoparticles and poly(dimethylsiloxane), and
wherein the size of the gold nanoparticles is 10 nm to 100 nm.

9 . The detection kit of claim 8 , wherein the wavelength of light exposed to the photothermal material is 500 nm to 600 nm.

10 . The detection kit of claim 1 , wherein a sample is fixed to the first sample area, and
wherein the sample comprises a detection material that receives heat from the first photothermal area and causes a chemical reaction at a predetermined temperature.

11 . The detection kit of claim 10 , wherein the sample further comprises an indicator whose color changes according to pH, and
wherein the detection material is a material whose pH changes when a chemical reaction occurs.

12 . The detection kit of claim 1 , further comprising:
a first protection member which is laminated on one side of the detection member; and a second protection member which is laminated on the other side of the detection member.

13 . The detection kit of claim 12 , wherein the first protection member is formed to have a larger area than one surface of the detection member such that a first edge part is disposed outside the one surface of the detection member, and
wherein the second protection member is formed to have a larger area than the other surface of the detection member such that a second edge part is disposed outside the other surface of the detection member.

14 . The detection kit of claim 13 , wherein the first edge part of the first protection member is bonded to the second edge part of the second protection member such that the detection member is disposed between the first protection member and the second protection member in a sealed state.

15 . The detection kit of claim 1 , further comprising:
a second photothermal area which is formed on the other side of the detection member and fixes a photothermal material that generates heat exposed to light; and a second sample area which is disposed adjacent to the second photothermal area to receive heat from the second photothermal area when light is irradiated onto the second photothermal area.

16 . The detection kit of claim 15 , wherein the first sample fixed to the first sample area comprises a detection material whose pH changes while receiving heat from the first photothermal area to cause a chemical reaction at a predetermined temperature and an indicator whose color changes according to pH; and
wherein the second sample fixed to the second sample area comprises an indicator whose color changes according to pH.

17 . A detection apparatus, comprising:
the detection kit according to claim 1 ; an enclosure-shaped housing with a through-hole formed on one side; a holder which is detachably coupled to one surface of the housing and supported by the detection kit; a light irradiation module which is fixed to the inside of the housing and irradiates light onto the first photothermal area through the through-hole; and a power supply module which is built into the housing and supplies power to the light irradiation module.

18 . The detection apparatus of claim 17 , wherein the holder is provided with an insertion groove into which the detection kit can be slid in one direction and inserted such that the first photothermal area of the detection kit is disposed adjacent to the through-hole.

19 . The detection apparatus of claim 17 , wherein the detection kit further comprises:
a second photothermal area which is formed on the other side of the detection member and fixes a photothermal material that generates heat exposed to light; and a second sample area which is disposed adjacent to the second photothermal area to receive heat from the second photothermal area when light is irradiated onto the second photothermal area, and wherein the light irradiation module comprises: a first light irradiation member which irradiates light onto the first photothermal area; and a second light irradiation member which irradiates light onto the second photothermal area.

20 . A method for manufacturing a detection kit which manufactures the detection kit according to claim 1 , comprising:
a photothermal material fixing step of fixing the photothermal material to the first photothermal area of the detection member; a punching step of removing a central portion of the first photothermal area; a first protection member attaching step of attaching a first protection member to one surface of the detection member; and a first sample area forming step of forming the first sample area at the central portion of the penetrated first photothermal area.

21 . The method of claim 20 , wherein the photothermal material fixing step comprises:
a photothermal material applying step of applying the photothermal material to one surface of the first photothermal area of the detection member; a photothermal material absorbing step of absorbing the photothermal material into the first photothermal area and reaching the other surface of the first photothermal area; and a photothermal material curing step of curing the photothermal material in a state where the photothermal material is absorbed in the first photothermal area.

22 . The method of claim 20 , wherein in the first sample area forming step, the first sample area is formed by inserting a member identical to the detection member into the central portion of the penetrated first photothermal area.

23 . The method of claim 20 , further comprising:
a sample fixing step of fixing a sample to the first sample area; and a second protection member attaching step of attaching a second protection member to the other surface of the detection member.

24 . The method of claim 23 , wherein in the second protection member attaching step, a first edge part of the first protection member and a second edge part of the second protection member are bonded such that the detection member is disposed between the first protection member and the second protection member in a sealed state.

Assignees

• Nat Univ Pukyong Ind Univ Coop Found

Inventors

• Shin Joong Ho
• Kim Ye Lin
• Kwak Minseok

Classifications

• G01N21/8483 Primary

  Investigating reagent band (test-element handling not specific to a test method G01N33/4875; analytical elements specific to chemical analysis of biological material G01N33/52; autometer with reagent band G01N35/04) · CPC title

• G01N21/78

  producing a change of colour · CPC title

• G01N1/44

  Sample treatment involving radiation, e.g. heat · CPC title

• G01N1/36

  Embedding or analogous mounting of samples · CPC title

• G01N33/48

  Biological material, e.g. blood, urine (G01N33/02, G01N33/26, G01N33/44, G01N33/46 take precedence); Haemocytometers (counting blood corpuscules distributed over a surface by scanning the surface G06M11/02) · CPC title