Process for the removal and recovery of hesperaloe extractives

Patent metadata

Field	Value
Publication number	US-2023233644-A1
Application number	US-202118011310-A
Country	US
Kind code	A1
Filing date	Jun 21, 2021
Priority date	Jun 19, 2020
Publication date	Jul 27, 2023
Grant date	—

Abstract

Disclosed are soluble extractives prepared from non-woody plants of the genus Hesperaloe and processes for preparing the same. The extracts preferably comprise at least one saponin. In certain instances, the process includes providing biomass derived from non-woody plants of the genus Hesperaloe, milling the biomass, washing the biomass with a solvent to yield a crude extract and optionally further purifying the crude extract by filtration to remove water insoluble compositions such as fibers, fines, epidermal debris and lipids. Preferably, the composition extracted from Hesperaloe comprises 25(27)-dehydrofucreastatin, 5(6),25(27)-disdehydroyuccaloiside C, 5(6)-disdehydroyuccaloiside, C, furcreastatin, yuccaloiside, or a mixture thereof.

First claim

1 . A composition extracted from a non-woody plant of the genus Hesperaloe , the extract comprising 25(27)-dehydrofucreastatin, 5(6),25(27)-disdehydroyuccaloiside C, 5(6)-disdehydroyuccaloiside C, furcreastatin, yuccaloiside C, or a mixture thereof.

2 . The composition of claim 1 wherein the extract is water soluble.

3 . The composition of claim 1 comprising at least about 5 wt % saponins, based upon the bone dry weight of the composition.

4 . The composition of claim 1 comprising from about 5 to about 40 wt % saponins, based upon the bone dry weight of the composition.

5 . The composition of claim 1 further comprising inorganic salts, saccharides, lipids, or proteins.

6 . The composition of claim 1 comprising 25(27)-dehydrofucreastatin, 5(6),25(27)-disdehydroyuccaloiside C, 5(6)-disdehydroyuccaloiside C, furcreastatin and yuccaloiside C.

7 . The composition of claim 1 wherein the saponins comprise an aglycone selected from kammogenin, manogenin, gentrogenin, hecogenin, tigogenin, sarsapogenin, chlorogenin and gitogenin, and at least one glycosidic selected from glucose, xylose, rhamnose, arabinose and galactose.

8 . The composition of claim 1 further comprising at least about 10 wt % inorganic salts and at least about 5 wt % saccharides, based upon the bone dry weight of the composition.

9 . The composition of claim 1 further comprising from about 10 to about 25 wt % inorganic salts and from about 5 to about 10 wt % saccharides, based upon the bone dry weight of the composition.

10 . The composition of claim 1 comprising at least 90 wt % water soluble solids, based upon the bone dry weight of the composition.

11 . The composition of claim 1 comprising less than about 2.0 wt % water insoluble solids, based upon the bone dry weight of the composition.

12 . The composition of claim 1 wherein the composition is substantially free from water insoluble solids.

13 . The composition of claim 1 wherein the composition is substantially free from hydrophobic solids, proteins, and lipids.

14 . The composition of claim 1 wherein the non-woody plant is Hesperaloe funifera, Hesperaloe noctuma, Hesperaloe parviflora, Hesperaloe chiangii , or a mixture thereof.

15 . A process for preparing an extract from the biomass of non-woody plants of the genus Hesperaloe comprising the steps of cutting the biomass, milling the biomass, extracting the biomass with a solvent, and collecting the crude extract.

16 . The process of claim 15 wherein the solvent is an aqueous solvent having a pH from about 6.0 to about 8.0.

17 . The process of claim 15 further comprising the step of imbibing the biomass with the crude extract.

18 . The process of claim 15 further comprising the step of depithing the biomass.

19 . The process of claim 15 wherein the non-woody plant is Hesperaloe funifera, Hesperaloe noctuma, Hesperaloe parviflora, Hesperaloe chiangii , or a mixture thereof.

20 . The process of claim 15 further comprising the step of concentrating the crude extract and wherein the concentrated crude extract comprises from about 50 to about 95% solids by weight.

21 . The process of claim 15 wherein the solvent is water having a pH from about 6.0 to about 7.5 and a temperature from about 40 to about 90° C.

22 . The process of claim 15 wherein the step of cutting the biomass comprises passing the biomass through a hammer mill to yield a cut biomass having a particle size ranging from about 1.0 to about 10.0 mm.

23 . The process of claim 15 further comprising processing the crude extract to separate the water soluble fraction from the water insoluble fraction.

24 . The process of claim 15 wherein the crude extract comprises from about 5 to about 30 wt % saponins, based upon the bone dry weight of the water soluble solids.

25 . The process of claim 16 wherein the crude extract comprises 25(27)-dehydrofucreastatin, 5(6),25(27)-disdehydroyuccaloiside C, 5(6)-disdehydroyuccaloiside C, furcreastatin, yuccaloiside C, or a mixture thereof.

26 . The process of claim 15 wherein the crude extract comprises at least about 10 wt % inorganic salts and at least about 5 wt % saccharides, based upon the bone dry weight of the water soluble solids.

27 . The process of claim 15 wherein the crude extract comprises from about 10 to about 25 wt % inorganic salts and from about 5 to about 10 wt % saccharides, based upon the bone dry weight of the water soluble solids.

28 . The process of claim 15 wherein the crude extract comprises less than about 2.0 wt % water insoluble solids.

29 . The process of claim 15 further comprising the step of filtering the crude extract to separate the water insoluble solids from the water soluble solids.

30 . The process of claim 15 further comprising the steps of mixing the crude extract with a salt and a solvent to form a first solution, adjusting the pH of the first solution to about 7.0 or less, adding at least one phosphate to the first solution to form a precipitate and removing the precipitate.

31 . The process of claim 15 further comprising the steps of mixing the crude extract with a solvent comprising an alcohol and water, defatting the crude extract with a non-polar solvent, and extracting saponins from the defatted crude extract with a polar solvent.

Assignees

• Kimberly Clark Co

Inventors

• Wei Ning
• Shannon Thomas G
• Thompson Brent M
• Wideman Gregory J

Classifications

• A61K36/88 Primary

  Liliopsida (monocotyledons) · CPC title

• B01D11/0288 Primary

  Applications, solvents · CPC title

• B01D9/0054

  Use of anti-solvent · CPC title

• B01D11/0284

  Multistage extraction · CPC title

• A61K31/7048

  having oxygen as a ring hetero atom, e.g. leucoglucosan, hesperidin, erythromycin, nystatin {, digitoxin or digoxin} · CPC title