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Modified Microorganisms and Production Method of Compounds
US2023227865A1 · US · A1
| Field | Value |
|---|---|
| Publication number | US-2023227865-A1 |
| Application number | US-202318152907-A |
| Country | US |
| Kind code | A1 |
| Filing date | Jan 11, 2023 |
| Priority date | Jan 19, 2022 |
| Publication date | Jul 20, 2023 |
| Grant date | — |
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Abstract
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A modified microorganism containing a genetic modification that suppresses a transcription factor that controls expression of pyruvate dehydrogenase, in which the microorganism has and a production pathway of a C6 compound, and in which the C6 compound is at least one compound selected from the group consisting of adipic acid, hexamethylenediamine, 1,6-hexanediol, 6-aminohexanoic acid, 6-amino-1-hexanol, 6-hydroxyhexanoic acid, 3-oxoadipic acid, 3-hydroxyadipic acid, and 2,3-dehydroadipic acid.
First claim
Opening claim text (preview).
1 . A modified microorganism comprising a genetic modification that suppresses a transcription factor that controls expression of pyruvate dehydrogenase, wherein the microorganism has a production pathway of a C6 compound, and wherein the C6 compound is at least one compound selected from the group consisting of adipic acid, hexamethylenediamine, 1,6-hexanediol, 6-aminohexanoic acid, 6-amino-1-hexanol, 6-hydroxyhexanoic acid, 3-oxoadipic acid, 3-hydroxyadipic acid, and 2,3-dehydroadipic acid. 2 . The modified microorganism according to claim 1 , wherein the genetic modification that suppresses a transcription factor that controls expression of pyruvate dehydrogenase is one or more selected from: a modification that suppresses expression of a gene encoding the transcription factor. and a modification that reduces an activity of the transcription factor in comparison to a non-reduced strain. 3 . The modified microorganism according to claim 1 , wherein the modified microorganism belongs to a genus selected from the group consisting of Escherichia, Bacillus, Corynebacterium, Arthrobacter, Brevibacterium, Clostridium, Zymomonas, Pseudomonas, Burkholderia, Streptomyces, Rhodococcus, Synechocystis, Alkalihalobacillus, Saccharomyces, Schizosaccharomyces, Yarrowia, Candida, Pichia, and Aspergillus. 4 . The modified microorganism according to claim 1 wherein the modified microorganism is Escherichia coli. 5 . The modified microorganism according to claim 1 wherein the transcription factor that controls expression of pyruvate dehydrogenase is PdhR. 6 . A method for producing a C6 compound, the method comprising a culture step of culturing the modified microorganism according to claim 1 .
Assignees
Inventors
Classifications
- C12P7/44Primary
Polycarboxylic acids · CPC title
- C12N9/0008Primary
acting on the aldehyde or oxo group of donors (1.2) · CPC title
Pyruvate dehydrogenase (acetyl-transferring) (1.2.4.1) · CPC title
polyhydric · CPC title
Amines; Imines · CPC title
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- What does patent US2023227865A1 cover?
- A modified microorganism containing a genetic modification that suppresses a transcription factor that controls expression of pyruvate dehydrogenase, in which the microorganism has and a production pathway of a C6 compound, and in which the C6 compound is at least one compound selected from the group consisting of adipic acid, hexamethylenediamine, 1,6-hexanediol, 6-aminohexanoic acid, 6-amino-…
- Who is the assignee on this patent?
- Asahi Chemical Ind
- What technology area does this patent fall under?
- Primary CPC classification C12P7/44. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Thu Jul 20 2023 00:00:00 GMT+0000 (Coordinated Universal Time) (A1). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).