Improved demineralization of fermentation broths and purification of fine chemicals such as oligosaccharides

Patent metadata

Field	Value
Publication number	US-2023227487-A1
Application number	US-202118008684-A
Country	US
Kind code	A1
Filing date	Jun 10, 2021
Priority date	Jun 12, 2020
Publication date	Jul 20, 2023
Grant date	—

Abstract

The present invention relates to a method for improved demineralization of fermentation broths, including the steps of providing a solution comprising one or more oligosaccharides, carrying out a first membrane filtration and preferably being a microfiltration or ultrafiltration, a second membrane filtration of the permeate of the first membrane filtration and a first nanofiltration step; with a sub-step of concentration and/or a sub-step of diafiltration. Moreover, the present invention is directed to an improved purification of fine chemicals from a fermentation broth.

First claim

1 - 17 . (canceled)

18 . A method for the demineralization of a solution comprising one or more fine chemicals, wherein the method comprises the steps of:
a. providing the solution comprising one or more oligosaccharides; b. optionally adjusting the pH to the desired value below 7 or less by adding at least one acid to the solution comprising at least one oligosaccharide, c. optional decolourisation step, d. an optional incubation step, e. carrying out a first membrane filtration, f. a second membrane filtration of the permeate of the first membrane filtration, g. optional decolourisation step of the permeate of the second membrane filtration, h. a first nanofiltration step; with a sub-step of concentration and/or a sub-step of diafiltration.

19 . The method according to claim 18 , wherein steps b) and c) are performed.

20 . The method according to claim 18 , wherein no cation exchange or anion ion exchange is carried out.

21 . The method according to claim 18 , wherein in step h), a sub-step of concentration and a sub-step of diafiltration is carried out.

22 . The method according to claim 18 , wherein the concentration sub-step of step h) is performed so that the concentration factor is at least 3 or more.

23 . The method according to claim 18 , wherein the diafiltration sub-step of step h) is performed so that the diafiltration factor is from 2.5 to 3.5.

24 . The method according to claim 18 , wherein the nanofiltration membrane has a NaCl retention between 5 to 30%.

25 . A method for purification of one or more fine chemicals from a solution comprising biomass and one or more oligosaccharides, comprising the steps of
i. providing a solution comprising biomass and one or more oligosaccharides, ii. setting the pH value of the solution below 7 or less by adding at least one acid to the solution comprising biomass and the at least one oligosaccharide, iii. decolourizing the solution at least in part by adding an adsorbing agent to the solution comprising biomass and one or more oligosaccharides, iv. optionally an incubation step, v. carrying out a membrane filtration also called herein the first membrane filtration and typically being a microfiltration or ultrafiltration so as to separate the biomass from the solution comprising the one or more oligosaccharides; vi. optionally carrying out at least one second or further membrane filtration with the permeate of the first membrane filtration; vii. optionally carrying out a decolourization step; viii. carrying out a nanofiltration with the permeate of the membrane filtration antedating this step viii, either with the permeate of the first or the second or any further membrane filtration; ix. optionally a decolourization step; x. optionally a second nanofiltration with the retentate of the nanofiltration of the previous nanofiltration step viii, wherein the nanofiltration membrane used is a different one to the one in the previous nanofiltration step; xi. optionally a third or further nanofiltration with a membrane differing from the one of the previous nanofiltration step.

26 . The method of claim 25 , with subsequent to step xi further processing of the retentate of the previous nanofiltration step by any of the following steps is conducted:
a. carrying out a decolourization step, and / or b. carrying out a demineralization step, and / or c. carrying out an electrodialysis and / or reverse osmosis and / or concentration step and / or a decolourization step; and / or d. carrying out a simulated moving bed chromatography, and / or a solidification step creating a solid oligosaccharide product, and / or a spray drying of the oligosaccharide followed by drying as desired.

27 . The method according to claim 26 wherein a demineralization step is conducted after the last one of the one or more nanofiltrations of steps viii, x and xi, wherein the demineralization is performed by ion exchange and wherein further the throughput of the demineralisation step is increased by a factor of at least 2 or more compared to the throughput of an identical ion exchange step without the one or more nanofiltrations of steps viii, x and xi preceding it.

28 . The method according to claim 18 , wherein the pH value of the solution is lowered to a pH value in the range of 3.0 to 5.5.

29 . The method according to claim 18 , wherein said adsorbing agent is added in an amount in the range of 0.5% to 3% by weight.

30 . The method according to claim 18 , wherein said first membrane filtration is carried out as cross-flow microfiltration or cross-flow ultrafiltration.

31 . The method according to claim 18 , wherein said first membrane filtration is carried out at a temperature of the solution in the range of 8° C. to 55° C.

32 . The method according to claim 18 , further comprising carrying out a second membrane filtration with the solution comprising oligosaccharides obtained by the first membrane filtration.

33 . The method according to claim 31 , wherein said second membrane filtration is carried out at a temperature of the solution being in the range of 5° C. to 15° C.

34 . The method according to claim 18 , wherein said at least one oligosaccharide comprises human milk oligosaccharide.

Assignees

• Basf Se

Inventors

• Billing Johan Fredrik
• Kappert Emiel Jan
• Wong Chung Huan
• Malisz Jacek

Classifications

• C07H1/08 Primary

  from natural products · CPC title

• A23C9/142

  by dialysis, reverse osmosis or ultrafiltration (A23C9/144 takes precedence) · CPC title

• A23C9/146

  by ion-exchange · CPC title

• C07H3/06

  Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages · CPC title

• C12P19/04

  Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds · CPC title