Device for evaluating state of sample, system containing same, method for evaluating state of sample and lactate dehydrogenase used therefor

Patent metadata

Field	Value
Publication number	US-2023213499-A1
Application number	US-202117801033-A
Country	US
Kind code	A1
Filing date	Feb 18, 2021
Priority date	Feb 21, 2020
Publication date	Jul 6, 2023
Grant date	—

Abstract

An object of the present invention is to provide a device for evaluating a state of interstitial fluid, blood, urine, tears, sweat, saliva of human and non-human organisms, foods and drinks, brewed product, etc., a system, a program, and an evaluation method using these.The present invention is to provide a device for evaluating a state of a sample which comprises an action part for allowing lactate dehydrogenase to act on a sample, and a sensor for sensing the state of the sample on which lactate dehydrogenase is allowed to act, a system, a program, a method for evaluating the state of the sample using these, and an enzyme to be used therefor.

First claim

1 .- 16 . (canceled)

17 . A device for monitoring state of a sample, wherein the device comprises an action part for allowing
(A) flavin-dependent lactate dehydrogenase which maintains about 20% or more of an initial activity when it is elapsed at 37° C. for 10 days in a solution, (B) flavin-dependent lactate dehydrogenase which maintains about 20% or more of an initial activity when it is elapsed at 37° C. for 3 days or longer in a solution, or (C) flavin-dependent lactate dehydrogenase which maintains about 20% or more of an initial activity when it is elapsed at 37° C. for 15 hours or longer in a solution to act on a sample.

18 . A system which comprises the device according to claim 17 which further comprises an output part, and the output part is connected to a data processing unit.

19 . A method for monitoring a state of a sample which uses the device according to claim 17 .

20 . A lactate dehydrogenase which comprises an amino acid sequence at positions 110 to 502 in an amino acid sequence shown by SEQ ID NO:4 or an amino acid sequence having 70% or more of identity thereof, an amino acid sequence at positions 113 to 505 in an amino acid sequence shown by SEQ ID NO:7 or an amino acid sequence having 70% or more of identity thereof, an amino acid sequence at positions 112 to 503 in an amino acid sequence shown by SEQ ID NO:10 or an amino acid sequence having 70% or more of identity thereof, or an amino acid sequence at positions 102 to 499 in an amino acid sequence shown by SEQ ID NO:12 or an amino acid sequence having 70% or more of identity thereof.

21 . The lactate dehydrogenase according to claim 20 , which has an amino acid sequence represented by SEQ ID NO:4, SEQ ID NO:7, SEQ ID NO:10, or SEQ ID NO:12 or an amino acid sequence having 70% or more of identity thereof.

22 . Nucleic acid which encodes lactate dehydrogenase according to claim 20 .

23 . Nucleic acid which encodes lactate dehydrogenase according to claim 21 .

24 . Host cells having nucleic acid according to claim 22 .

25 . Host cells having nucleic acid according to claim 23 .

26 . A method for producing lactate dehydrogenase, which comprises culturing the host cells according to claim 24 .

27 . A method for producing lactate dehydrogenase, which comprises culturing the host cells according to claim 25 .

28 . A method for evaluating a state of a sample, which comprises
i) a step of bringing lactate dehydrogenase according to claim 20 into contact with the sample, and ii) a step of measuring lactic acid.

29 . A method for evaluating a state of a sample, which comprises
i) a step of bringing lactate dehydrogenase according to claim 21 into contact with the sample, and ii) a step of measuring lactic acid.

Assignees

• Kikkoman Corp

Inventors

• Masakari Yosuke
• Inamoto Chiaki
• Nakamura Miki
• KAN Eiichiro
• Ichiyanagi Atsushi
• HIRAGUCHI Haruka
• Suzuki Ryota

Classifications

• C12Y101/05

  with a quinone or similar compound as acceptor (1.1.5) · CPC title

• C12Y101/01027

  L-Lactate dehydrogenase (1.1.1.27) · CPC title

• C12Q1/32 Primary

  involving dehydrogenase · CPC title

• G01N33/50 Primary

  Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing (measuring or testing processes involving enzymes or microorganisms, compositions or test papers therefor; processes for forming such compositions, condition responsive control in microbiological or enzymological processes C12Q) · CPC title

• G01N2333/904

  acting on CHOH groups as donors, e.g. glucose oxidase, lactate dehydrogenase (1.1) · CPC title