Flowcell cartridge with floating seal bracket

Patent metadata

Field	Value
Publication number	US-2023191416-A1
Application number	US-202318167836-A
Country	US
Kind code	A1
Filing date	Feb 11, 2023
Priority date	Jan 3, 2017
Publication date	Jun 22, 2023
Grant date	—

Abstract

A cartridge for use with chemical or biological analysis systems, as well as methods of using the same, is provided. The cartridge may include a floating microfluidic plate that is held in the cartridge using one or more floating support brackets that incorporate gaskets that may seal against fluidic ports on the microfluidic plate. The floating support brackets may include indexing features that may align the microfluidic plate with the seals.

First claim

1 . A method comprising:
rotating a first clamp arm about a first axis in a plane parallel to a plane of a surface of a chuck of a receiver, the rotating first clamp arm contacting a surface of a microfluidic plate of a flow cell cartridge disposed on the chuck of the receiver, the microfluidic plate comprising a fluidic port proximate a first gasket fluidic port of a first gasket assembly of the flow cell cartridge, the microfluidic plate moveable longitudinally and laterally within a predetermined range of movement relative to a frame of the flow cell cartridge; translating a first longitudinal indexing pin offset longitudinally from the chuck to contact a portion of the flow cell cartridge, the translating longitudinal indexing pin causing the flow cell cartridge to be compressed between the first longitudinal indexing pin and a second longitudinal indexing pin; and engaging a first fluidic port indexing feature of the flow cell cartridge with a first alignment hole of a fluidic port block of the receiver, the fluidic port block moveable at least one of longitudinally or laterally relative to the chuck of the receiver, the fluidic port block comprising a receiver fluidic port, the receiver fluidic port aligned with the first gasket fluidic port of the first gasket assembly of the flow cell cartridge responsive to the first fluidic port indexing feature of the flow cell cartridge being inserted into the first alignment hole of the fluidic port block.

2 . The method of claim 1 , further comprising translating the first clamp arm laterally along the first axis in the plane parallel to the plane of the surface of the chuck.

3 . The method of claim 2 , wherein the first clamp arm laterally translates the microfluidic plate relative to the chuck of the receiver.

4 . The method of claim 2 , wherein the translating of the first clamp arm laterally along the first axis occurs during at least some of the rotating of the first clamp arm about the first axis.

5 . The method of claim 4 , wherein the rotating of the first clamp arm, at least in part, serves to first align the microfluidic plate with the first gasket assembly and the fluidic port block and then clamp the aligned microfluidic plate in place relative to the chuck.

6 . The method of claim 1 , wherein the flow cell cartridge comprises a first support bracket comprising the first gasket assembly, a first indexing feature, and a second indexing feature, the first indexing feature of the first support bracket abutting the microfluidic plate at a first edge of the microfluidic plate and the second indexing feature of the first support bracket abutting the microfluidic plate at a second edge of the microfluidic plate when the first gasket fluidic port of the first gasket assembly aligns with the fluidic port of the microfluidic plate, wherein the first support bracket floats relative to the microfluidic plate and the frame of the flow cell cartridge.

7 . The method of claim 6 , wherein the first longitudinal indexing pin engages the first support bracket when the first longitudinal indexing pin is caused to contact the portion of the flow cell cartridge, thereby compressing the first indexing feature of the first support bracket against the first edge of the microfluidic plate.

8 . The method of claim 7 , wherein the flow cell cartridge further comprises a second support bracket comprising a second gasket assembly having another first indexing feature, the first indexing feature of the second support bracket proximate a third edge of the microfluidic plate opposite the first edge of the microfluidic plate and the second indexing feature of the second support bracket proximate the second edge of the microfluidic plate of the microfluidic plate, wherein the second support bracket floats relative to the microfluidic plate and the frame of the flow cell cartridge.

9 . The method of claim 8 , further comprising causing the second support bracket to engage the second longitudinal indexing pin while the first longitudinal indexing pin is caused to engage the first support bracket, thereby compressing the first indexing feature of the second support bracket against the third edge of the microfluidic plate.

10 . The method of claim 9 , wherein the second longitudinal indexing pin is fixedly mounted with respect to the chuck.

11 . The method of claim 10 , wherein:
the frame has an opening with a first interior edge, at least a portion of the microfluidic plate is positioned within the opening, and the second indexing feature of the first gasket assembly and the second indexing feature of the second gasket assembly are interposed between the first interior edge and the second edge of the microfluidic plate, the method further comprising moving the first interior edge of the frame towards the second edge of the microfluidic plate so as to cause the second indexing feature of the first gasket assembly and the second indexing feature of the second gasket assembly to contact both the first interior edge of the frame and the second edge of the microfluidic plate.

12 . The method of claim 11 , wherein the receiver has a first lateral indexing pin positioned proximate a fourth edge of the microfluidic plate opposite the second edge of the microfluidic plate, the method further comprising moving the first interior edge of the frame towards the first lateral indexing pin so as to cause the fourth edge of the microfluidic plate to be pressed against the first lateral indexing pin.

13 . The method of claim 11 , wherein:
the frame has a first clamp arm slot located along a second interior edge of the frame extending in a direction transverse to the first interior edge of the frame, and the first clamp arm presses against a side of the first clamp arm slot to move the first interior edge of the frame towards the second edge of the microfluidic plate.

14 . The method of claim 13 , further comprising rotating a second clamp arm about a second axis parallel to the first axis, the rotating second clamp arm also contacting the surface of the microfluidic plate, wherein:
the frame has a second clamp arm slot located along a third interior edge of the frame extending in a direction transverse to the first interior edge of the frame and located opposite the second interior edge of the frame, and the second clamp arm presses against a side of the second clamp arm slot to also move the first interior edge of the frame towards the second edge of the microfluidic plate.

15 . The method of claim 6 , wherein the first fluidic port indexing feature is located on the first support bracket.

16 . The method of claim 15 , further comprising moving the fluidic port block along a direction perpendicular to, and towards, the microfluidic plate to cause the first fluidic port indexing feature of the flow cell cartridge to engage with the first alignment hole of the fluidic port block.

17 . The method of claim 16 , wherein:
the first support bracket further includes a second fluidic port indexing feature, the first alignment hole is circular in cross-section and the fluidic port block has at least a second alignment hole that is obround in cross-section, and moving the fluidic port block along the direction perpendicular to, and towards, the microfluidic plate also causes the second fluidic port indexing feature to engage with the second alignment hole, and the engagement of the first fluidic port indexing feature and the second fluidic port indexing feature with the first alignment hole and the second alignment hole, respectively, serves to both positionally

Assignees

• Illumina Inc

Inventors

• Kaplan David Elliott
• De Ruyter Anthony John
• Kelley Richard Alan
• Kumar Ashish

Classifications

• B01L2300/041

  Connecting closures to device or container · CPC title

• B01L2200/0689

  Sealing · CPC title

• B01L9/527 Primary

  for microfluidic devices, e.g. used for lab-on-a-chip · CPC title

• B01L2300/0609

  Holders integrated in container to position an object · CPC title

• B01L2200/027

  for microfluidic devices · CPC title