Method for detection of a bottom of at least one well

Patent metadata

Field	Value
Publication number	US-2023152342-A1
Application number	US-202218056626-A
Country	US
Kind code	A1
Filing date	Nov 17, 2022
Priority date	Nov 17, 2021
Publication date	May 18, 2023
Grant date	—

Abstract

A method for detection of a bottom of a well of a multiwell plate for a pipetting device is disclosed. The pipetting device includes a pipetting head configured for being coupled to a plurality of pipetting tips. A force sensor configured for measuring a resistance force depending on a force on a pipetting tip exerted by the bottom is used. The method includes measuring the resistance force during movement of one of the pipetting tips from a start position downstream towards the bottom of the well and stopping the movement at a bottom position. The method further includes storing the bottom position together with a corresponding logical position of the well in the multiwell plate in a database. Further disclosed is a pipetting device, a laboratory instrument for processing and/or analyzing a sample and a computer program and a computer-readable storage medium for performing the method.

First claim

1 . A method for detection of a bottom of at least one well of a multiwell plate for a pipetting device, wherein the pipetting device comprises at least one pipetting head configured for being coupled to a plurality of pipetting tips, wherein the method comprises using at least one force sensor configured for measuring a resistance force depending on a force on a pipetting tip exerted by the bottom, wherein the method comprises the following steps:
a) measuring the resistance force during movement of one of the pipetting tips from a start position downstream towards the bottom of the well and stopping the movement at a bottom position, wherein the bottom position is a position where a predefined resistance force is reached; and b) storing the bottom position together with a corresponding logical position of the well in the multiwell plate in at least one database.

2 . The method of claim 1 , wherein the method comprises considering the stored bottom position for at least one subsequent pipetting procedure.

3 . The method of claim 2 , wherein the method comprises determining whether a stored bottom position is available for a logical position in the database, wherein, in case a stored bottom position is available, the method proceeds with a subsequent pipetting procedure, otherwise method steps a) to b) are performed.

4 . The method of claim 1 , wherein steps a) and b) are repeated in case of new consumables and/or new pipetting tips.

5 . The method of claim 1 , wherein the force sensor comprises a load cell configured for generating a sensor signal proportional to the resistance force exerted on the pipetting tip.

6 . The method of claim 1 , wherein the method is performed during at least one of aspiration, sip and spit, and dispensing.

7 . The method of claim 1 , wherein the method is performed during aspiration and/or sip and spit, wherein the method further comprises, subsequent to step a), moving the pipetting tip upwards until the resistance force has decreased to a predefined residual force, and moving the pipetting tip upwards with a predefined distance to its final position for aspiration and/or sip and spit.

8 . The method of claim 1 , wherein the method comprises surveillance of the force sensor, wherein the force sensor is moved to a defined position where the force sensor experiences at least one resistance force generated from at least one mechanical spring.

9 . The method according to claim 8 , wherein two different forces are applied to the force sensor.

10 . A pipetting device comprising a pipetting head configured to be coupled to a plurality of pipetting tips, wherein the pipetting device is configured for performing the method for detection of a bottom of at least one well of a multiwell plate, wherein the pipetting device comprises at least one force sensor configured for measuring a resistance force depending on a force on the pipetting tip exerted by the bottom, wherein the pipetting device comprises at least one control unit configured for executing the following steps:
i. measuring the resistance force during movement of one of the pipetting tips from a start position downstream towards the bottom of the well and stopping the movement at a bottom position, wherein the bottom position is a position where a predefined resistance force is reached; ii. storing the bottom position together with a corresponding logical position of the well in the multiwell plate in at least one database.

11 . The pipetting device of claim 10 , wherein the control unit is configured to consider the stored bottom position for at least one subsequent pipetting procedure.

12 . The pipetting device of claim 11 , wherein the control unit is configured to determine whether a stored bottom position is available for a logical position in the database, wherein, in case a stored bottom position is available, the control unit is configured to execute a subsequent pipetting procedure, otherwise steps i) to ii) are performed.

13 . A laboratory instrument for processing and/or analyzing a sample, wherein the laboratory instrument comprises at least one pipetting device according to claim 10 , wherein the laboratory instrument is one or more of a pre-analytical, an analytical or a post-analytical instrument.

14 . The laboratory instrument of claim 13 , wherein the laboratory instrument comprises a force control check station, wherein the force control check station is configured for surveillance of the force sensor, wherein the force control check station is configured for exerting at least one resistance force on the force sensor generated from at least one mechanical spring.

15 . The laboratory instrument according to claim 14 , wherein two different forces are applied to the force sensor.

Assignees

• Roche Molecular Systems Inc

Inventors

• Gut Raphael
• Hermann Pius
• Meier Alexander
• Birkner Patrick
• Ettel Christian
• Spescha Roger
• Wang Pengfei
• Durrer Simon

Classifications

• B01L3/02

  Burettes; Pipettes · CPC title

• G01N35/1009 Primary

  Characterised by arrangements for controlling the aspiration or dispense of liquids · CPC title

• B01L3/021

  Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids · CPC title

• B01L2300/0829

  Multi-well plates; Microtitration plates · CPC title

• B01L2200/143

  Quality control, feedback systems · CPC title