Method, Computer Program Product and System for Sequencing a Prepared DNA Strand and a Sensor Unit

Patent metadata

Field	Value
Publication number	US-2023151419-A1
Application number	US-202217960490-A
Country	US
Kind code	A1
Filing date	Oct 5, 2022
Priority date	Nov 16, 2021
Publication date	May 18, 2023
Grant date	—

Abstract

A method of sequencing a prepared DNA strand includes providing the prepared DNA strand with nucleotides of the types dATP, dCTP, dGTP and/or dTTP, wherein at least one of the types of the nucleotides comprises a predetermined magnetic label. The method further includes placing the prepared DNA strand within a measuring range of a sensor unit comprising an operatively connected magneto-optical transducer unit and an optical sensor and optically exciting the magneto-optical transducer unit. The method continues with acquiring at least one value indicative of a fluorescence signal of the magneto-optical transducer unit, and assigning the acquired value to the at least one type of the nucleotides comprising the predetermined magnetic label.

First claim

1 . A method of sequencing a prepared DNA strand, comprising:
providing the prepared DNA strand with nucleotides of the types dATP, dCTP, dGTP and/or dTTP, wherein at least one of the types of the nucleotides comprises a predetermined magnetic label; placing the prepared DNA strand within a measuring range of a sensor unit comprising an operatively connected magneto-optical transducer unit and an optical sensor; optically exciting the magneto-optical transducer unit; acquiring at least one value indicative of a fluorescence signal of the magneto-optical transducer unit; and assigning the acquired value to the at least one type of the nucleotides comprising the predetermined magnetic label.

2 . The method according to claim 1 , further comprising:
subjecting the magneto-optical transducer unit to microwave radiation.

3 . The method according to claim, further comprising:
subjecting the magneto-optical transducer unit to a static magnetic field; and subjecting the magneto-optical transducer unit to an RF magnetic field.

4 . The method according to claim 3 , wherein a gradient magnetic field is used as the static magnetic field.

5 . The method according to claim 1 , further comprising:
carrying out a spin polarization.

6 . The method according to claim 1 , wherein:
the sensor unit is stationary and the prepared DNA strand is shifted in a predetermined manner; the prepared DNA strand is stationary and the sensor unit is shifted in a predetermined manner relative to the prepared DNA strand; or the prepared DNA strand and the sensor unit are both shifted in a predetermined manner.

7 . A computer program product comprising at least one non-transitory computer-readable storage medium having computer-executable program code portions stored therein, the computer-executable program code portions comprising program code instructions configured to:
place a prepared DNA strand with nucleotides of the types dATP, dCTP, dGTP and/or dTTP, wherein at least one of the types of the nucleotides comprises a predetermined magnetic label, within a measuring range of a sensor unit comprising an operatively connected magneto-optical transducer unit and an optical sensor; optically excite the magneto-optical transducer unit; acquire at least one value indicative of a fluorescence signal of the magneto-optical transducer unit; and assign the acquired value to the at least one type of the nucleotides comprising the predetermined magnetic label.

8 . A system for sequencing a prepared DNA strand, wherein the prepared DNA strand comprises nucleotides of the types dATP, dCTP, dGTP and/or dTTP, wherein at least one of the types of the nucleotides comprises a predetermined magnetic label, comprising:
a sensor unit comprising an operatively connected magneto-optical transducer unit and an optical sensor, with a light source configured to optically excite the magneto-optical transducer unit, wherein the optical sensor is configured to acquire at least one value indicative of a fluorescence signal of the magneto-optical transducer unit, and the system is configured to assign the acquired at least one value to the at least one type of nucleotide comprising the predetermined magnetic label.

9 . The system according to claim 8 , wherein the system further comprises:
a microwave source configured to apply microwave radiation to the magneto-optical transducer unit.

10 . The system according to claim 8 , further comprising:
a magnet configured to apply a static magnetic field to the magneto-optical transducer unit; and a microwave source configured to apply an RF magnetic field to the magneto-optical transducer unit.

11 . The system according to claim 10 , wherein the magnet is configured to provide a gradient magnetic field as the static magnetic field.

12 . The system according to claim 8 , wherein the system is further configured to carry out a spin polarization.

13 . The system according to claim 8 , wherein the system is configured to:
shift the prepared DNA strand in a predetermined manner while maintaining the sensor unit stationary; shift the sensor unit relative to the prepared DNA strand in a predetermined manner while maintaining the prepared DNA strand stationary; or shift both the prepared DNA strand and the sensor unit in a predetermined manner.

Assignees

• Bosch Gmbh Robert

Inventors

• Brenneis Andreas
• Stuerner Felix Michael
• Hoffmann Jochen
• Fomina Nadezda
• Roelver Robert
• Fuchs Tino
• Grumaz Christian

Classifications

• C12Q1/6806

  Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay (C12Q1/6804 takes precedence) · CPC title

• C12Q1/6869 Primary

  Methods for sequencing · CPC title

• G01N21/6428

  Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" (in vivo A61B5/00; immunoassay G01N33/53) · CPC title

• G01N21/645

  Specially adapted constructive features of fluorimeters · CPC title

• G01N21/6486

  Measuring fluorescence of biological material, e.g. DNA, RNA, cells (G01N21/6428 takes precedence) · CPC title