Elite event ee-gm5 and methods and kits for identifying such event in biological samples

Patent metadata

Field	Value
Publication number	US-2023151381-A1
Application number	US-202218058537-A
Country	US
Kind code	A1
Filing date	Nov 23, 2022
Priority date	Dec 22, 2016
Publication date	May 18, 2023
Grant date	—

Abstract

The invention provides specific transgenic soybean plants, plant material and seeds, characterized in that these products harbor a specific nematode resistance and herbicide tolerance transformation event at a specific location in the soybean genome. Tools are also provided which allow rapid and unequivocal identification of the event in biological samples.

First claim

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9 . A soybean plant, cell, plant part, seed or progeny thereof, comprising a nucleic acid molecule of comprising a nucleotide sequence selected from:
(a) a nucleotide sequence essentially similar to the sequence of any one of SEQ ID No. 1, 3 or 5 or to the sequence of any one of SEQ ID No. 2, 4, or 6, or the complement of said sequences; (b) a nucleotide sequence with at least 99% sequence identity to the nucleotide sequence of SEQ ID No. 3, 4, 5, 6, 24 or 25, or the complement thereof; (c) the nucleotide sequence of any one of SEQ ID No. 1 or 3 or SEQ ID No. 2 or 4, or the complement of said sequences; or (d) the nucleotide sequence of SEQ ID No. 1 or 3 and SEQ ID No. 2 or 4, or the complement thereof.

10 . A soybean plant, cell, plant part, seed or progeny thereof, comprising in its genome the nucleotide sequence of SEQ ID No. 3 and the nucleotide sequence of SEQ ID No. 4 or the nucleotide sequence of SEQ ID No. 5 and the nucleotide sequence of SEQ ID No. 6, or the nucleotide sequence of SEQ ID No. 24 and the nucleotide sequence of SEQ ID No. 25.

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12 . The plant, cell, plant part, seed or progeny of claim 10 which also comprises the nucleotide sequence of SEQ ID No. 7 and 9, or the nucleotide sequence of SEQ ID No. 23 from nucleotide position 1114 to nucleotide position 8572.

13 . A soybean plant, cell, part, or seed, each comprising in its genome elite event EE-GM5, wherein said elite event is the genetic locus comprising an inserted T-DNA containing a chimeric HPPD-4 protein-encoding gene and a chimeric Cry14Ab-1 protein-encoding gene, and comprising nucleotide sequence of SEQ ID No. 3 and the nucleotide sequence of SEQ ID No. 4.

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17 . A soybean product produced from the soybean plant, cell, part, or seed of claim 9 .

18 . The soybean product of claim 17 , which is or comprises soybean meal, ground seeds, soybean flour or soybean flakes.

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20 . A method for producing a soybean product, comprising obtaining the soybean plant, or cell, part, seed or progeny of claim 9 , and producing such soybean product therefrom.

21 . The method of claim 20 , wherein said soybean product is or comprises soybean meal, ground seeds, soybean flour or soybean flakes.

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25 . A method for protecting emerging soybean plants of claim 9 from competition by weeds, comprising treating a field to be planted with said soybean plants with an HPPD inhibitor herbicide, before the soybean plants are planted or the seeds are sown, followed by planting or sowing of said soybean plants or seeds in said pre-treated field.

26 . The process of claim 23 , wherein said HPPD inhibitor herbicide is isoxaflutole, topramezone or mesotrione.

27 . A method for producing a soybean plant resistant to SCN and/or tolerant to HPPD inhibitor herbicides, comprising introducing resistance to SCN and/or tolerance to HPPD inhibitor herbicides into the genome of a soybean plant by crossing a first soybean plant lacking a Cry14Ab-1-encoding gene and lacking an HPPD-4-encoding gene with the soybean plant of claim 9 , and selecting a progeny plant resistant to SCN and/or tolerant to HPPD inhibitor herbicides.

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31 . A method for producing a soybean plant or seed comprising elite event EE-GM5, comprising crossing a plant according to claim 9 with another soybean plant, and planting the seed comprising EE-GM5 obtained from said cross.

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63 . A method for reducing yield loss in a field to be planted with soybean plants, particularly a field that contains, contained or is expected to contain nematodes or nematode eggs such as SCN or Pratylenchus or reniform nematodes or their eggs, comprising planting or sowing of soybean plants or seeds of claim 9 .

64 . A method for increasing yield of soybean plants when planted in a field containing nematodes or nematode eggs such as SCN or Pratylenchus or reniform nematodes or eggs thereof, comprising planting or sowing of soybean plants or seeds of claim 9 .

65 . A method for producing a soybean plant or seed tolerant to an HPPD inhibitor herbicide, such as isoxaflutole, topramezone or mesotrione, or for producing a soybean plant or seed tolerant to nematodes, such as SCN or Pratylenchus or reniform nematodes, or for producing a soybean plant or seed tolerant to an HPPD inhibitor herbicide, such as isoxaflutole, topramezone or mesotrione, and tolerant to nematodes, such as SCN or Pratylenchus or reniform nematodes, comprising introducing into the genome of a soybean plant or seed elite soybean a nucleic acid molecule as recited in claim 9 ,
and optionally treating said plant or seed with an HPPD inhibitor herbicide, such as isoxaflutole, topramezone or mesotrione, or optionally treating the field in which said plant or seed will be planted with an HPPD inhibitor herbicide, such as isoxaflutole, topramezone or mesotrione, and planting said plant or seed in said pre-treated field.

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Assignees

• BASF Agricultural Solutions Seed US LLC

Inventors

• Moser Hal
• Mccarville Michael
• Buyse Maxim
• Slabbinck Filip
• Beilinson Vadim
• Kleven Thomas W
• Daum Julia
• Aartsen Wendy
• Habex Veerle

Classifications

• C12Q1/6895

  for plants, fungi or algae · CPC title

• C12Q2600/158

  Expression markers · CPC title

• Y02A40/146

  Genetically Modified [GMO] plants, e.g. transgenic plants · CPC title

• A01N35/06

  containing keto or thioketo groups as part of a ring, e.g. cyclohexanone, quinone; Derivatives thereof, e.g. ketals · CPC title

• C12N15/11

  DNA or RNA fragments; Modified forms thereof (DNA or RNA not used in recombinant technology, C07H21/00); {Non-coding nucleic acids having a biological activity} · CPC title