Light-sheet photonic-force optical coherence elastography

Patent metadata

Field	Value
Publication number	US-2023072425-A1
Application number	US-202117759851-A
Country	US
Kind code	A1
Filing date	Feb 1, 2021
Priority date	Jan 31, 2020
Publication date	Mar 9, 2023
Grant date	—

Abstract

Disclosed are devices and techniques based on optical coherence tomography (OCT) technology in combination with optical actuation. A system for providing optical actuation and optical sensing can include an optical coherence tomography (OCT) device that performs optical imaging of a sample based on optical interferometry from an optical sampling beam interacting with an optical sample and an optical reference beam; an OCT light source to provide an OCT imaging beam into the OCT device which splits the OCT imaging beam into the optical sampling beam and the optical reference beam; and a light source that produces an optical actuation beam that is coupled along with the optical sampling beam to be directed to the sample to actuate particles or structures in the sample so that the optical imaging captures information of the sample under the optical actuation.

First claim

1 . A system for providing optical actuation and optical sensing, comprising:
an optical coherence tomography (OCT) device that performs optical imaging of a sample based on optical interferometry from an optical sampling beam interacting with an optical sample and an optical reference beam; an OCT light source to provide an OCT imaging beam into the OCT device which splits the OCT imaging beam into the optical sampling beam and the optical reference beam; a light source that produces an optical actuation beam that is coupled along with the optical sampling beam to be directed to the sample to actuate particles or structures in the sample so that the optical imaging captures information of the sample under the optical actuation; an optical beam shaping module located in an optical path between the light source and the sample to shape the optical actuation beam into a light-sheet to illuminate a region of the sample; a fluorescent excitation light source to produce a fluorescent excitation light to the sample to cause the sample to emit fluorescent light; and a fluorescent detection module located to receive the emitted fluorescent light from the sample to measure one or more properties of the sample from the emitted fluorescent light, wherein the fluorescent detection module includes:
different optical filters to receive different portions of the emitted fluorescent light from the sample to filter the received different portions to generate filtered fluorescent beams at different center optical wavelengths, respectively; and
different optical detectors located to receive the filtered fluorescent beams at different center optical wavelengths, respectively, to capture sample information from the filtered fluorescent beams at different center optical wavelengths.

2 . The system as in claim 1 , wherein the optical actuation beam is modulated at a modulation frequency.

3 . The system as in claim 2 , wherein the optical actuation beam is modulated in amplitude or power.

4 . The system as in claim 1 , further comprising an OCT detection and processing module that is configured to process optical imaging information from OCT device output based on varying mechanical properties of different sample materials to distinguish one sample material from another.

5 . The system as in claim 1 , further comprising an OCT detection and processing module that is configured to process optical imaging information from OCT device output based on varying optical scattering properties of different sample materials to distinguish one sample material from another.

6 . (canceled)

7 . The system as in claim 1 , wherein the fluorescent detection module includes an imaging sensor to capture an optical image carried by the emitted fluorescent light from the sample in addition to an optical image produced by the OCT device.

8 . (canceled)

9 . The system as in claim 1 , wherein the OCT light source is configured to provide an OCT imaging beam at an optical wavelength different from an optical wavelength of the optical actuation beam.

10 . The system as in claim 1 , further comprising a scanner that is configured to scan the optical sampling beam over the sample in performing the optical imaging without scanning the optical actuation beam.

11 . The system as in claim 1 , further comprising a beam guiding optics module that is configured to direct the optical actuation beam to spatially overlap with the optical sampling beam over the sample in performing the optical imaging.

12 . The system as in claim 1 , wherein the optical beam shaping module includes optical lenses that include one or more cylindrical lenses.

13 . The system as in claim 1 , further comprising:
an optical combiner device located downstream from the optical beam shaping module to receive the optical actuation beam from the optical beam shaping module and the optical sampling beam, the optical combiner device structured and operable to combine the optical actuation beam and the optical sampling beam to propagate along a common optical path to the sample.

14 . A method of quantifying mechanical properties of a sample, comprising:
operating a first light source emitting light in a first optical band of optical wavelengths to produce
a sampling beam optically interacting with the sample and
a reference beam,
operating a phase-sensitive low-coherence optical interferometry device to obtain optical images of the sample from optical interference of the sampling beam and the reference beam, operating a second light source to produce an optical actuation beam at a second optical wavelength different from the wavelengths in the first band, modulating the optical actuation beam at a modulation frequency, intensity or power, using a modulation waveform, and operating an optical beam shaping module located in an optical path between the second light source and the sample to shape the optical actuation beam into a light sheet, directing the optical actuation beam shaped into the light-sheet to the sample to actuate mechanical movements of microparticle probes so that the optical images capture first information of the mechanical movements of the microparticle probes, and using the first information to obtain second information of the mechanical properties of the sample, wherein the first information includes an amplitude of movements of the microparticle probes and a phase shift of movements of the microparticle probes with respect to the modulation waveform.

15 - 18 . (canceled)

19 . The method of claim 14 , wherein the second information includes components of a complex shear modulus.

20 . A method of quantifying mechanical properties of a sample, comprising:
operating a first light source emitting light in a first optical band of optical wavelengths to produce a sampling beam optically interacting with the sample and a reference beam; operating a phase-sensitive low-coherence optical interferometry device to obtain optical images of the sample from optical interference of the sampling beam and the reference beam; operating a second light source to produce an optical actuation beam at a second optical wavelength different from the wavelengths in the first band; operating an optical beam shaping module located in an optical path between the second light source and the sample to shape the optical actuation beam into a light sheet; directing the optical actuation beam shaped into the light-sheet to the sample to actuate mechanical movements of microparticle probes so that the optical images capture first information of the mechanical movements of the microparticle probes; and using the first information to obtain second information of the mechanical properties of the sample, wherein the shaping comprises modifying an optical phase of the optical actuation beam along an axis.

21 . A method of quantifying mechanical properties of a sample, comprising:
operating a first light source emitting light in a first optical band of optical wavelengths to produce a sampling beam optically interacting with the sample and a reference beam; operating a phase-sensitive low-coherence optical interferometry device to obtain optical images of the sample from optical interference of the sampling beam and the reference beam, operating a second light source to produce an optical actuation beam at a second optical wavelength different from the wavelengths in the first band; operating an optical beam shaping module located in an optical path between the second light source and the sample to shape the optical actu

Assignees

• Univ Cornell

Inventors

• Adie Steven G
• Lin Yuechuan
• Leartprapun Nichaluk

Classifications

• G01N21/6486

  Measuring fluorescence of biological material, e.g. DNA, RNA, cells (G01N21/6428 takes precedence) · CPC title

• G01B9/02012

  using temporal intensity variation · CPC title

• G01B9/02034

  characterised by particularly shaped beams or wavefronts · CPC title

• G01B9/02091 Primary

  Tomographic interferometers, e.g. based on optical coherence · CPC title

• G01B9/02044

  Imaging in the frequency domain, e.g. by using a spectrometer · CPC title