Cell culture device

Patent metadata

Field	Value
Publication number	US-2022389360-A1
Application number	US-202017754994-A
Country	US
Kind code	A1
Filing date	Oct 12, 2020
Priority date	Oct 17, 2019
Publication date	Dec 8, 2022
Grant date	—

Abstract

A cell culture device is provided. A cell culture device according to an exemplary embodiment of the present invention comprises: a housing which has a plurality of through-holes formed through at least one surface thereof to allow carbon dioxide to be introduced thereinto from the outside and includes an inner space filled with a medium for culturing cells; a plurality of supporters which are arranged in the inner space in multiple steps while being spaced at an interval from each other so as to culture cells and are provided in a plate-shape having a predetermined area; and a porous member which is attached to one surface of the housing so as to cover the plurality of through-holes, prevents the medium filled in the inner space from leaking to the outside, and allows carbon dioxide to be introduced into the inner space from the outside.

First claim

1 . A cell culture device comprising:
a housing in which a plurality of through-holes are formed in at least one surface to allow carbon dioxide to enter from the outside and which has an inner space filled with a culture medium for cell culture; a plurality of support bodies which are disposed in multiple stages at predetermined intervals from each other in the inner space for cell culture and which are provided in the shape of a plate having a predetermined area; and a porous member which is attached to one surface of the housing to cover the plurality of through-holes and is configured to prevent external leakage of the culture medium filled in the inner space and allow carbon dioxide to enter the inner space from the outside.

2 . The cell culture device of claim 1 , wherein the support body includes a motif-coated, plate-shaped nanofiber membrane.

3 . The cell culture device of claim 2 , wherein the support body includes the motif-coated, plate-shaped nanofiber membrane and a support member which is attached to one surface of the nanofiber membrane via an adhesive layer to support the nanofiber membrane.

4 . The cell culture device of claim 3 , wherein the support body includes a plurality of holes formed to pass through the support member to facilitate passage of carbon dioxide entering the inner space through the plurality of through-holes.

5 . The cell culture device of claim 4 , wherein a first area which is a sum of areas of the plurality of holes is relatively smaller than a second area which is an area excluding the first area from the entire area of the support member.

6 . The cell culture device of claim 1 , wherein the support body is a plasma-treated, plate-shaped film member.

7 . The cell culture device of claim 6 , wherein the plasma-treated, plate-shaped film member includes a plurality of holes formed to pass therethrough to facilitate passage of carbon dioxide entering the inner space through the plurality of through-holes.

8 . The cell culture device of claim 7 , wherein a first area which is a sum of areas of the plurality of holes is relatively smaller than a second area which is an area excluding the first area from the entire area of the plasma-treated, plate-shaped film member.

9 . The cell culture device of claim 1 , wherein the plurality of support bodies maintain a state in which two support bodies adjacent to each other are spaced apart via a spacer disposed in the inner space.

10 . The cell culture device of claim 9 , wherein:
the spacer includes two guide members inserted into the inner space so that one surface of one guide member and one surface of the other guide member face each other; and the two guide members include a plurality of slot grooves formed to be recessed in a longitudinal direction so that end sides of the support bodies are able to be fitted to the surfaces facing each other.

11 . The cell culture device of claim 10 , wherein the two guide members are disposed so that one surface of each guide member comes into contact with one of two inner surfaces that face each other among inner surfaces of the housing.

12 . The cell culture device of claim 1 , wherein the porous member is a membrane treated to be water-repellent.

13 . The cell culture device of claim 1 , wherein the housing includes at least one culture medium inlet disposed at a side portion of the housing to allow a culture medium supplied from the outside to enter the inner space.

14 . The cell culture device of claim 1 , further comprising a blower fan coupled to the housing to suction carbon dioxide present outside the housing and supply the suctioned carbon dioxide into the plurality of through-holes.

15 . The cell culture device of claim 14 , wherein:
the housing includes a concave portion formed to be recessed a predetermined depth inward in one surface; the plurality of through-holes are formed to pass through a bottom surface of the concave portion; a lid member on which the blower fan is mounted is coupled to the housing to cover an open upper portion of the concave portion; and the blower fan is disposed to be spaced a predetermined distance apart from the plurality of through-holes.

16 . The cell culture device of claim 15 , wherein carbon dioxide present outside the housing enters the concave portion through the blower fan and then is dispersed and injected into the inner space through the plurality of through-holes.

Assignees

• Amogreentech Co Ltd

Inventors

• Park Hee Sung
• Seo In Yong
• Lee Seoung Hoon
• Han Kyung Gu
• Jang Seon Ho
• Kim Jae Yun
• Lee Su Yeon
• Kim Ji Young
• Lee Hyo Jung

Classifications

• C12M25/06

  Plates; Walls; Drawers; Multilayer plates · CPC title

• C12M23/24 Primary

  Gas permeable parts · CPC title

• C12M23/20 Primary

  Material Coatings (immunocoatings C12M25/00) · CPC title

• C12M29/20

  Degassing; Venting; Bubble traps (means for collection or storage of gas C12M23/36; gas collection apparatus for laboratory use B01L5/02) · CPC title

• C12M25/02

  Membranes; Filters (filters or filtration in general B01D24/00-B01D41/00) · CPC title