Methdos for detecting estradiol by mass spectrometry

Patent metadata

Field	Value
Publication number	US-2022326260-A1
Application number	US-202217735879-A
Country	US
Kind code	A1
Filing date	May 3, 2022
Priority date	Nov 27, 2007
Publication date	Oct 13, 2022
Grant date	—

Abstract

Provided are methods for determining the amount of estradiol in a sample using mass spectrometry. The methods generally involve ionizing estradiol in a sample and detecting and quantifying the amount of the ion to determine the amount of estradiol in the sample.

First claim

1 .- 53 . (canceled)

54 . A method for determining the amount of estradiol in a sample, the method comprising:
(a) purifying estradiol from a sample by liquid chromatography; (b) ionizing the purified estradiol from the sample to produce one or more ions detectable by tandem mass spectrometry; and (c) detecting the amount of the estradiol ion(s) by tandem mass spectrometry, wherein said one or more ions comprise an ion with a mass/charge ratio of 183.10±0.5 or 145.10±0.5; wherein the amount of the estradiol ion(s) is related to the amount of estradiol in said test sample.

55 . The method of claim 54 , wherein the liquid chromatography is high performance liquid chromatography (HPLC).

56 . The method of claim 54 , wherein the purifying step comprises high turbulence liquid chromatography (HTLC) followed by high performance liquid chromatography (HPLC).

57 . The method of claim 54 , wherein an agent is added to the sample in an amount sufficient to free estradiol from a protein prior to the ionizing step.

58 . The method of claim 57 , wherein the agent is formic acid.

59 . The method of claim 57 , wherein the agent is a salt.

60 . The method of claim 59 , wherein the salt is ammonium sulfate.

61 . The method of claim 54 , wherein the method has a limit of quantitation less than or equal to 25 pg/mL.

Assignees

• Quest Diagnostics Invest Inc

Inventors

• Goldman Mildred M
• Clarke Nigel J
• Reitz Richard E

Classifications

• G01N30/7233

  interfaced to liquid or supercritical fluid chromatograph (interfaces in general for introducing or extracting samples to be analysed with specially adapted mass spectrometer, see H01J49/04) · CPC title

• H01J49/004

  Combinations of spectrometers, tandem spectrometers, e.g. MS/MS, MSn · CPC title

• H01J49/0431

  for liquid samples (interfaces to liquid chromatographs G01N30/7233) · CPC title

• G01N33/6851

  Methods of protein analysis involving laser desorption ionisation mass spectrometry · CPC title

• G01N33/743 Primary

  Steroid hormones · CPC title