Method of producing sulfur-containing amino acid or derivative thereof

Patent metadata

Field	Value
Publication number	US-2022315963-A1
Application number	US-202017597007-A
Country	US
Kind code	A1
Filing date	Jun 26, 2020
Priority date	Jun 28, 2019
Publication date	Oct 6, 2022
Grant date	—

Abstract

A method of producing sulfur-containing amino acids or derivatives of the sulfur-containing amino acids.

First claim

What is claimed is:

1 . A method of producing a sulfur-containing amino acid or a derivative of the sulfur-containing amino acid, the method comprising culturing a genetically modified microorganism in a culture medium including thiosulfate,
wherein the microorganism includes genetic modification to increase activity of a protein encoded by ssuD gene compared to a non-modified microorganism.

2 . The method of claim 1 , wherein the protein encoded by ssuD gene has thiosulfate reductase activity.

3 . The method of claim 1 , wherein the protein encoded by ssuD gene includes an amino acid sequence having at least 80% homology with SEQ ID NO: 43.

4 . The method of claim 1 , wherein the microorganism is a microorganism belonging to the genus Corynebacterium or the genus Escherichia sp.

5 . The method of claim 4 , wherein the microorganism is Corynebacterium glutamicum, Corynebacterium callunae, Corynebacterium deserti, Corynebacterium crenatum , or Escherichia coli.

6 . The method of claim 1 , further comprising recovering the sulfur-containing amino acid and the derivative of the sulfur-containing amino acid from the microorganism or the culture medium.

7 . The method of claim 1 , wherein genetic modification to increase the activity of the protein is achieved by i) increasing a copy number of a polynucleotide encoding the protein in a cell, ii) replacing an expression regulatory region of a polynucleotide encoding the protein with a sequence with stronger activity, iii) modifying an initiation codon or 5′-UTR of a polynucleotide encoding the protein, iv) modifying a nucleotide sequence on a chromosome to enhance the activity of the protein, v) introducing a foreign polynucleotide expressing the activity of the protein or a codon optimized variant polynucleotide of the polynucleotide encoding the protein, or vi) a combination thereof.

8 . The method of claim 1 , wherein the sulfur-containing amino acid or the derivative of the sulfur-containing amino acid comprises at least one selected from the group consisting of methionine, cysteine, cystine, lanthionine, homocysteine, homocystine, homolanthionine, and taurine.

9 . A microorganism producing a sulfur-containing amino acid and a derivative of the sulfur-containing amino acid and comprising genetic modification to increase activity of a protein encoded by ssuD gene compared to a non-modified microorganism.

10 . The microorganism of claim 9 , wherein the microorganism produces the sulfur-containing amino acid or the derivative of the sulfur-containing amino acid using thio sulfate as a sulfur source.

11 . The microorganism of claim 9 , wherein genetic modification to increase the activity of the protein is achieved by i) increasing a copy number of a polynucleotide encoding the protein in a cell, ii) replacing an expression regulatory region of a polynucleotide encoding the protein with a sequence with stronger activity, iii) modifying an initiation codon or 5′-UTR of a polynucleotide encoding the protein, iv) modifying a nucleotide sequence on a chromosome to enhance the activity of the protein, v) introducing a foreign polynucleotide expressing the activity of the protein or a codon optimized variant polynucleotide of the polynucleotide encoding the protein, or vi) a combination thereof.

12 . A composition for producing a sulfur-containing amino acid or a derivative of the sulfur-containing amino acid,
wherein the composition comprises: a microorganism including genetic modification to increase activity of a protein encoded by ssuD gene compared to a non-modified microorganism, or a culture thereof; and thiosulfate.

13 . (canceled)

14 . (canceled)

Assignees

• Cj Cheiljedang Corp

Inventors

• Choi Sol
• Kim Hee Ju
• Rho Jin Ah
• Lee Jin Nam
• Lee Han Hyoung
• Lee Sun Young
• Kim Sang Jun
• Shim Jihyun

Classifications

• C12N15/77

  for Corynebacterium; for Brevibacterium · CPC title

• C12Y108/05

  with a quinone or similar compound as acceptor (1.8.5) · CPC title

• C07K14/245

  Escherichia (G) · CPC title

• C12P13/12 Primary

  Methionine; Cysteine; Cystine · CPC title

• C12N9/0051 Primary

  acting on a sulfur group of donors (1.8) · CPC title