Device for detecting analytes in a sample, and methods of use thereof

Patent metadata

Field	Value
Publication number	US-2022283116-A1
Application number	US-202017632613-A
Country	US
Kind code	A1
Filing date	Aug 3, 2020
Priority date	Aug 3, 2019
Publication date	Sep 8, 2022
Grant date	—

Abstract

Devices for detecting an analyte in a sample suspected of containing the analyte, are provided. The devices include bio-functional, nanostructured, isoporous membranes (BNIM) integrated organic electrochemical transistor (OECT), herein BNIM-OECT, for the rapid and sensitive detection of the presence of an analyte of interest, in a sample, for example, a biological sample. The membrane (i.e., BNIM) is physically separated from the OECT channel therefore the electronic device can be used multiple times. The isoporous membrane is functionalized to include a binding partner for the analyte being detected. The BNIM-OECT can be used for disease detection, by functionalizing the BNIM-OECT with a binding partner to an analyte associated with the disease, applying a collected biological sample to the BNIM-OECT. A decrease in channel current as a result of analyte binding to its binding partner on the isoporous membrane indicates the presence of the analyte in the sample.

First claim

1 . A device for detecting an analyte in a sample suspected of containing the analyte, comprising an isoporous membranes (BNIM) and an organic electrochemical transistor (OECT), wherein the BNIM comprises a binding partner specific for the analyte.

2 . The device of claim 1 , wherein the OECT comprises a source electrode, a drain electrode, a channel, and a gate electrode, wherein the source and drain electrodes are electrically connected by the channel.

3 . The device of claim 2 further comprising a supporting substrate, wherein the source electrode, the drain electrode, and the channel are patterned on the supporting substrate.

4 . The device of claim 3 , wherein the substrate is selected from the group consisting of glass, silicon, paper, coated paper, resin-coated paper, paper laminates, paperboard, and corrugated board.

5 . The device of claim 2 , wherein the channel is made from a conducting polymer.

6 . The device of claim 5 , wherein the conducting polymer is selected from the group consisting of PEDOT:PSS, PEDOT-S, PEDOT:TOS, PEDOTOH:ClO 4 , PEDOT-co-PEDOTOH:ClO 4 , P3HT, PTHS, BBL, p(g2T-TT), PTHS − TMA + -co-P3HT, p(gNDI-g2T), p(g0T2-g6T2), and P-90.

7 . The device of claim 1 , wherein the isoporous membrane is made from the block copolymer poly(styrene-b-4-vinylpyridine).

8 . The device of claim 1 , wherein the binding partner specific for the analyte is smaller in size compared to the pore size of the isoporous surface of the membrane.

9 . The device of claim 1 , wherein the device comprises Congo Red or Thioflavin T.

10 . The device of claim 1 , wherein the isoporous membrane comprises an aptamer, optionally a peptide aptamer.

11 . The device of claim 1 , wherein the binding partner for the analyte is crosslinked to the isoporous surface of the membrane.

12 . The device of claim 1 , wherein the BNIM is placed above the channel and in contact with the source and drain electrodes.

13 . A method of detecting the presence of an analyte in a sample comprising
(i) contacting an electrolyte solution with the device of claim 1 , (ii) applying a drain voltage and a gate voltage, and (iii) measuring a drain current, transconductance, and/or response time, wherein a decrease in drain current, a decrease in transconductance, and/or an increased response time compared to those measured without the analyte indicates the presence of the analyte in the sample.

14 . The method of claim 13 , wherein the electrolyte solution comprises or is a biological sample.

15 . The method of claim 14 , wherein the biological sample is selected from the group consisting of blood, serum, cerebrospinal fluid, and saliva.

16 . The method of claim 13 , wherein the BNIM of the device is interposed vertically, in between the channel and the electrolyte solution.

17 . The method of claim 13 , wherein the BNIM of the device comprises Congo Red.

18 . The method of claim 13 , wherein the electrolyte solution comprises or is suspected of comprising amyloid beta aggregates.

19 . The method of claim 13 , wherein the electrolyte solution comprises a known concentration amyloid beta aggregates, the method further comprising generating a dose response curve.

20 . The method of claim 13 , comprising quantifying the amount of amyloid beta in a sample suspected of containing amyloid beta by comparing the decrease in current obtained as a result of binding of amyloid beta in a sample suspected of containing amyloid beta to Congo red on the isoporous membrane, to the dose response curve.

Assignees

• Univ King Abdullah Sci & Tech

Inventors

• Wustoni Shofarul
• Inal Sahika

Classifications

• G01N27/3335

  the membrane containing at least one organic component (G01N27/3271 takes precedence; aspects concerning the enzyme reagent in enzyme electrodes C12Q1/001) · CPC title

• G01N27/327

  Biochemical electrodes {, e.g. electrical or mechanical details for in vitro measurements} · CPC title

• G01N27/4145 Primary

  specially adapted for biomolecules, e.g. gate electrode with immobilised receptors · CPC title

• G01N27/44726

  using specific dyes, markers or binding molecules · CPC title

• G01N27/31

  Half-cells with permeable membranes, e.g. semi-porous or perm-selective membranes · CPC title