Detection chip and modification method therefor

Patent metadata

Field	Value
Publication number	US-2022274107-A1
Application number	US-202117631463-A
Country	US
Kind code	A1
Filing date	Mar 4, 2021
Priority date	Mar 19, 2020
Publication date	Sep 1, 2022
Grant date	—

Abstract

Disclosed are a detection chip and a modification method therefor. The method for modifying the detection chip comprises performing a surface film-forming treatment on a hydrophilic layer on a first substrate constituting the detection chip to form polydopamine film on a surface of the hydrophilic layer; and adopting a buffer solution containing sodium hyaluronate to perform a surface carboxylation treatment on the hydrophilic layer, on the surface of which the polydopamine film has been formed, in order to form a carboxyl-containing modification group on the surface of the hydrophilic layer.

First claim

1 . A modification method of a detection chip, comprising:
forming a polydopamine film on a surface of the hydrophilic layer by adopting a dopamine-containing buffer solution to perform surface film-forming treatment on the hydrophilic layer on a first substrate constituting the detection chip, wherein the hydrophilic layer covers sample application platforms located on the first substrate; and forming a carboxyl-containing modification group on a surface of the hydrophilic layer by adopting a buffer solution containing sodium hyaluronate to perform a surface carboxylation treatment on the hydrophilic layer with the polydopamine film.

2 . The modification method according to claim 1 , wherein a mass concentration ratio of the dopamine to the sodium hyaluronate is 1:1-1:5.

3 . The modification method according to claim 2 , wherein the adopting the dopamine-containing buffer solution to perform the surface film-forming treatment on the hydrophilic layer on the first substrate constituting the detection chip, comprises:
obtaining the buffer solution of the dopamine by weighing and taking 500 mg of dopamine to be dissolved in 250 mL of Tris-HCI, the Tris-HCI is of a pH value being 7-9 and a concentration being 10 mM; putting the first substrate with the hydrophilic layer into the buffer solution of the dopamine to be subjected to still standing at a room temperature for a reaction for 12 h-24 h.

4 . The modification method according to claim 2 , wherein the adopting the buffer solution containing the sodium hyaluronate to perform the surface carboxylation treatment on the hydrophilic layer with the polydopamine film, comprises:
obtaining the buffer solution of the sodium hyaluronate by weighing and taking 1.25 g of sodium hyaluronate to be dissolved in 250 mL of Tris-HCI, the Tris-HCI is of a pH value being 7-9 and a concentration being 10 mM; and putting the first substrate with the polydopamine film formed on the surface of the hydrophilic layer into the buffer solution of the sodium hyaluronate, to be subjected to still standing under a temperature condition of 20° C.-40° C. for a reaction for 12 h-24 h.

5 . The modification method according to claim 1 , wherein before the performing the surface film-forming treatment on the hydrophilic layer on the first substrate constituting the detection chip, the modification method further comprises:
forming the carboxyl-containing modification group on the surface of the hydrophilic layer by performing surface activation treatment on the hydrophilic layer on the first substrate.

6 . The modification method according to claim 5 , wherein the performing the surface activation treatment on the hydrophilic layer on the first substrate, comprises:
putting the first substrate with the hydrophilic layer into a piranha solution, to be soaked under a temperature condition of 70° C.-90° C. for 12 h-24 h, wherein the piranha solution comprises concentrated sulfuric acid and 30% hydrogen peroxide, and a volume ratio of the concentrated sulfuric acid to the 30% hydrogen peroxide is 1:3.

7 . The modification method according to claim 5 , wherein after the surface activation treatment, the surface film-forming treatment and the surface carboxylation treatment, following treatment is performed on the first substrate:
cleaning the first substrate for at least three times by adopting deionized water, and then blowing-drying the first substrate through nitrogen for standby application.

8 . The modification method according to claim 5 , wherein before the performing the surface activation treatment on the hydrophilic layer on the first substrate, the modification method further comprises:
ultrasonic-cleaning the first substrate with the hydrophilic layer by sequentially adopting acetone, ethyl alcohol and deionized water as a solution, and blow-drying ultrasonic-cleaned first substrate for standby application.

9 . The modification method according to claim 8 , wherein before the ultrasonic-cleaning the first substrate with the hydrophilic layer, the modification method further comprises:
forming the plurality of sample application platforms on the first substrate; and respectively forming the hydrophilic layers on the sample application platforms.

10 . The modification method according to claim 9 , wherein the respectively forming the hydrophilic layers on the sample application platforms, comprises:
depositing a silicon oxide layer with a thickness being 300 nm on a layer where the sample application platforms are located, by adopting a plasma enhanced chemical vapor deposition, under a temperature condition of 390° C.; and obtaining the hydrophilic layer by etching the silicon oxide layer, and reserving a silicon oxide layer covering a region where all the sample application platforms are located to.

11 . A detection chip, comprising:
a first substrate; sample application platforms, located on the first substrate; and a hydrophilic layer, located on the first substrate and covering the sample application platforms, wherein a surface of the hydrophilic layer comprises a carboxyl-containing modification group, and the carboxyl-containing modification group is obtained by adopting the modification method according to claim 1 .

12 . The detection chip according to claim 11 , further comprising:
a diversion dam, located on the first substrate, wherein the diversion dam extends in a first path and located between adjacent sample application platforms; and the hydrophilic layer covers the diversion dam, and a part, covering the diversion dam, of the hydrophilic layer and a part, covering the sample application platforms, of the hydrophilic layer are mutually independent.

13 . The detection chip according to claim 12 , wherein a height of the diversion dam in a direction perpendicular to the first substrate is greater than a height of the sample application platforms in the direction perpendicular to the first substrate.

14 . The detection chip according to claim 12 , further comprising:
a hydrophobic layer, located on the first substrate, wherein the sample application platforms and the diversion dam are located on the hydrophobic layer.

15 . The detection chip according to any one of claims 1244 , further comprising:
a second substrate, disposed opposite to the first substrate, and spaced from the first substrate so as to provide a detection space.

16 . The detection chip according to claim 15 , wherein at least one of the first substrate or the second substrate is a glass substrate.

17 . The detection chip according to claim 16 , further comprising:
sealant, located between the first substrate and the second substrate, and surrounds the diversion dam and the plurality of sample application platforms.

Assignees

• Boe Technology Group Co Ltd

Inventors

• Yin Yudan
• Yu Jing
• Liu Haonan
• Liu Zhukai

Classifications

• G01N33/543

  with an insoluble carrier for immobilising immunochemicals · CPC title

• C03C17/42 Primary

  at least one coating of an organic material and at least one non-metal coating · CPC title

• B01L3/502707 Primary

  characterised by the manufacture of the container or its components · CPC title

• C03C17/28

  with organic material (C03C17/34, C03C17/44 take precedence) · CPC title

• C03C17/30

  with silicon-containing compounds · CPC title