Detection chip and modification method therefor

Patent metadata

Field	Value
Publication number	US-2022274106-A1
Application number	US-202117631461-A
Country	US
Kind code	A1
Filing date	Mar 3, 2021
Priority date	Mar 19, 2020
Publication date	Sep 1, 2022
Grant date	—

Abstract

Disclosed are a detection chip and a modification method therefor. A surface activation treatment is performed on a hydrophilic layer of the detection chip, such that a hydroxy-containing modification group is formed on a surface of the hydrophilic layer; and a solution containing an oxy compound is then used to perform a surface epoxidation treatment on the hydrophilic layer in order to form an epoxy-containing modification group on the surface of the hydrophilic layer.

First claim

1 . A modification method of a detection chip, comprising:
performing surface activation treatment on a hydrophilic layer on a first substrate constituting the detection chip to form a hydroxy-containing modification group on a surface of the hydrophilic layer, wherein the hydrophilic layer covers sample application platforms located on the first substrate; and performing surface epoxidation treatment on the hydrophilic layer on which the hydroxy-containing modification group is formed, by using a solution containing an oxy compound to form an epoxy-containing modification group on a surface of the hydrophilic layer.

2 . The modification method according to claim 1 , wherein the performing the surface epoxidation treatment on the hydrophilic layer comprises:
placing the first substrate with the hydroxyl-containing modification group formed on the surface of the hydrophilic layer into a toluene solution with 0.5%-5% (v/v) of 3-glyceryl propyl trimethoxy silane, and performing airtight soaking at a temperature condition from a room temperature to 70° C. for 24 h-72 h.

3 . The modification method according to claim 2 , wherein the placing the first substrate with the hydroxyl-containing modification group formed on the surface of the hydrophilic layer into the toluene solution with 0.5%-5% (v/v) of 3-glyceryl propyl trimethoxy silane, and performing the airtight soaking at the temperature condition from the room temperature to 70° C. for 24 h-72 h, comprises:
placing the first substrate with the hydroxyl-containing modification group formed on the surface of the hydrophilic layer into a toluene solution with 3% (v/v) of 3-glyceryl propyl trimethoxy silane, and performing airtight soaking at 70° C. for 24 h.

4 . The modification method according to claim 1 , wherein the performing the surface activation treatment on the hydrophilic layer on the first substrate constituting the detection chip, comprises:
placing the first substrate with the hydrophilic layer into a piranha solution and soaking at a temperature condition of 70° C.-90° C. for 12 h-24 h, wherein the piranha solution comprises concentrated sulfuric acid and 30% hydrogen peroxide, and a volume ratio of the concentrated sulfuric acid to the 30% hydrogen peroxide is 1:3.

5 . The modification method according to claim 1 , wherein after the surface activation treatment and the surface epoxidation treatment, the first substrate is treated as follows:
washing the first substrate with deionized water at least twice; and blow-drying, after the washed first substrate is ultrasonically cleaned in the deionized water, with nitrogen for use.

6 . The modification method according to claim 1 , wherein before the performing the surface activation treatment on the hydrophilic layer on the first substrate constituting the detection chip, further comprising:
performing ultrasonic cleaning on the first substrate with the hydrophilic layer with acetone, ethanol, and deionized water as a solution in sequence, and blow-drying the finally ultrasonically cleaned first substrate with nitrogen for use.

7 . The modification method according to claim 6 , wherein before the performing the ultrasonic cleaning on the first substrate with the hydrophilic layer with acetone as the solution, further comprising:
forming a plurality of sample application platforms on the first substrate; and forming the hydrophilic layer on each of the sample application platforms respectively.

8 . The modification method according to claim 7 , wherein the forming the hydrophilic layer on each of the sample application platforms respectively, comprises:
depositing a silicon oxide layer with a thickness of 300 nm on a layer where each of the sample application platforms is located by using a plasma-enhanced chemical vapor deposition method at a temperature condition of 390° C.; and etching the silicon oxide layer, and reserving the silicon oxide layer covering a region where each of the sample application platforms is located to obtain the hydrophilic layer.

9 . A detection chip, comprising:
a first substrate; sample application platforms, located on the first substrate; and a hydrophilic layer, located on the first substrate and covering the sample application platforms, wherein a surface of the hydrophilic layer comprises a carboxyl-containing modification group; and the carboxyl-containing modification group is obtained by the modification method according to claim 1 .

10 . The detection chip according to claim 9 , further comprising:
diversion dams, located on the first substrate, extending along a first path and located between the adjacent sample application platforms; wherein the hydrophilic layer covers the diversion dams, and parts, covering the diversion dams, of the hydrophilic layer are independent of parts, covering the sample application platforms, of the hydrophilic layer.

11 . The detection chip according to claim 10 , wherein a height of the diversion dams in a direction perpendicular to the first substrate is greater than a height of the sample application platforms in the direction perpendicular to the first substrate.

12 . The detection chip according to claim 10 , further comprising:
a hydrophobic layer, located on the first substrate, wherein the sample application platforms and the diversion dams are located on the hydrophobic layer.

13 . The detection chip according to claim 10 , further comprising:
a second substrate, disposed opposite to the first substrate and spaced apart from the first substrate to provide a detection space.

14 . The detection chip according to claim 13 , wherein at least one of the first substrate or the second substrate is a glass substrate.

15 . The detection chip according to claim 13 , further comprising:
a sealant, located between the first substrate and the second substrate, and surrounding the diversion dams and the plurality of sample application platforms.

Assignees

• Boe Technology Group Co Ltd

Inventors

• Yin Yudan
• Yu Jing
• Liu Haonan
• Liu Zhukai

Classifications

• B01L2400/086

  using baffles or other fixed flow obstructions · CPC title

• B01L2300/161

  Control and use of surface tension forces, e.g. hydrophobic, hydrophilic · CPC title

• B01L2300/0819

  Microarrays; Biochips · CPC title

• B01L2200/0689

  Sealing · CPC title

• B01L3/502707 Primary

  characterised by the manufacture of the container or its components · CPC title