Detection chip and modification method therefor

Patent metadata

Field	Value
Publication number	US-2022274105-A1
Application number	US-202117631460-A
Country	US
Kind code	A1
Filing date	Mar 3, 2021
Priority date	Mar 19, 2020
Publication date	Sep 1, 2022
Grant date	—

Abstract

Disclosed are a detection chip and a modification method therefor. The modification method includes performing a surface activation treatment on a hydrophilic layer of the detection chip, such that a hydroxy-containing modification group is formed on a surface of the hydrophilic layer; then performing a surface amination treatment on the hydrophilic layer using a solution containing an amino compound, such that an amino-containing modification group is formed on the surface of the hydrophilic layer, and performing a surface carboxylation treatment on the hydrophilic layer using a solution containing an anhydride compound, such that a carboxyl-containing modification group is formed on the surface of the hydrophilic layer.

First claim

1 . A modification method for a detection chip, comprising:
performing surface activation treatment on a hydrophilic layer on a first substrate of the detection chip to form a hydroxyl-containing modification group on a surface of the hydrophilic layer, wherein the hydrophilic layer covers sample application platforms disposed on the first substrate; performing, with a solution containing an amino compound, surface amination treatment on the hydrophilic layer with the hydroxyl-containing modification group formed on the surface of the hydrophilic layer to form an amino-containing modification group on the surface of the hydrophilic layer; and performing, with a solution containing an anhydride compound, surface carboxylation treatment on the hydrophilic layer with the amino-containing modification group formed on the surface of the hydrophilic layer to form a carboxyl-containing modification group on the surface of the hydrophilic layer.

2 . The modification method according to claim 1 , wherein the performing, with the solution containing the amino compound, the surface amination treatment on the hydrophilic layer with the hydroxyl-containing modification group formed on the surface of the hydrophilic layer, comprises:
putting the first substrate having the hydrophilic layer with the hydroxyl-containing modification group formed on the surface of the hydrophilic layer into an ethanol solution or a toluene solution of 1%-2% (v/v) 3-aminopropyl triethoxysilane to be airtightly soaked for 12 h to 24 h in a normal temperature.

3 . The modification method according to claim 1 , wherein the performing, with the solution containing the anhydride compound, the surface carboxylation treatment on the hydrophilic layer with the amino-containing modification group formed on the surface of the hydrophilic layer, comprises:
putting the first substrate having the hydrophilic layer with the amino-containing modification group formed on the surface of the hydrophilic layer into an N,N-dimethylformamide saturated solution of butanedioic anhydride to be soaked for 24 h to 48 h under conditions of the normal temperature and a normal pressure.

4 . The modification method according to claim 1 , wherein the performing, with the solution containing the anhydride compound, the surface carboxylation treatment on the hydrophilic layer with the amino-containing modification group formed on the surface of the hydrophilic layer comprises:
putting the first substrate having the hydrophilic layer with the amino-containing modification group formed on the surface of the hydrophilic layer into an N,N-dimethylformamide saturated solution of butanedioic anhydride to be subjected to circulation water bath for 24 h to 48 h between 75 -90 .

5 . The modification method according to claim 1 , wherein the performing the surface activation treatment on the hydrophilic layer on the first substrate of the detection chip, comprises:
putting the first substrate having the hydrophilic layer into a piranha solution to be soaked for 12 h to 24 h between 70 -90 , wherein the piranha solution is composed of a concentrated sulphuric acid and 30% hydrogen peroxide, and a volume ratio of the concentrated sulphuric acid to the 30% hydrogen peroxide is 1:3.

6 . The modification method according to claim 1 , wherein after each of the surface activation treatment, the surface amination treatment and the surface carboxylation treatment, a following treatment is performed on the first substrate:
flushing the first substrate at least twice with deionized water; and performing ultrasonic cleaning treatment on the flushed first substrate in the deionized water; and drying the cleaned first substrate with nitrogen for standby application.

7 . The modification method according to claim 1 , before the performing the surface activation treatment on the hydrophilic layer on the first substrate of the detection chip, further comprising:
performing ultrasonic cleaning on the first substrate having the hydrophilic layer by sequentially using acetone, ethanol and the deionized water as a solution, and drying the ultrasonic-cleaned first substrate with nitrogen for standby application.

8 . The modification method according to claim 7 , before the performing the ultrasonic cleaning on the first substrate having the hydrophilic layer by using the acetone as the solution, further comprising:
forming a plurality of sample application platforms on the first substrate; and forming the hydrophilic layer on the plurality of sample application platforms respectively.

9 . The modification method according to claim 8 , wherein the forming the hydrophilic layer on the plurality of sample application platforms respectively, comprises:
depositing a silicon oxide layer with a thickness of 300 nm on a layer of the sample application platforms at 390 by using a plasma enhanced chemical vapor deposition method; and obtaining the hydrophilic layer by etching the silicon oxide layer and retaining the silicon oxide layer covering a region where each of the sample application platforms is located.

10 . A detection chip, comprising:
a first substrate; sample application platforms, on the first substrate; and a hydrophilic layer, on the first substrate and covering the sample application platforms, wherein a surface of the hydrophilic layer has a carboxyl-containing modification group, and
the carboxyl-containing modification group is obtained by using the modification method according to claim 1 .

11 . The detection chip according to claim 10 , further comprising:
a diversion dam, on the first substrate, extending along a first path and located between adjacent sample application platforms, wherein the hydrophilic layer covers the diversion dam; and a portion, covering the diversion dam, of the hydrophilic layer is independent of a portion, covering the sample application platforms, of the hydrophilic layer.

12 . The detection chip according to claim 11 , wherein a height of the diversion dam in a direction perpendicular to the first substrate is greater than a height of the sample application platforms in the direction perpendicular to the first substrate.

13 . The detection chip according to claim 11 , further comprising:
a hydrophobic layer, on the first substrate; wherein the sample application platforms and the diversion dam are all disposed on the hydrophobic layer.

14 . The detection chip according to claim 11 , further comprising:
a second substrate, wherein the second substrate is arranged in opposite to the first substrate and spaced from the first substrate so as to provide a detection space.

15 . The detection chip according to claim 14 , wherein at least one of the first substrate or the second substrate is a glass substrate.

16 . The detection chip according to claim 14 , further comprising:
a sealant, located between the first substrate and the second substrate and surrounding the diversion dam and the plurality of sample application platforms.

17 . The detection chip according to claim 14 , further comprising:
a sample inlet; a sample outlet; and a detection region; wherein the plurality of sample application platforms are located in the detection region and arranged in the plurality of columns; and the sample inlet and the sample outlet are distributed at two sides of the detection region in a column direction.

18 . The detection chip according to claim 14 , further comprising:
a positioning component, configured to cooperate with an optical detection device to re

Assignees

• Boe Technology Group Co Ltd

Inventors

• Yin Yudan
• Yu Jing
• Liu Haonan
• Liu Zhukai

Classifications

• B01L2400/086

  using baffles or other fixed flow obstructions · CPC title

• B01L2300/161

  Control and use of surface tension forces, e.g. hydrophobic, hydrophilic · CPC title

• B01L2300/0887

  Laminated structure · CPC title

• B01L2300/0819

  Microarrays; Biochips · CPC title

• B01L2200/0689

  Sealing · CPC title