Biological component treatment system, biological component treatment device, and cell culturing method

Patent metadata

Field	Value
Publication number	US-2022251495-A1
Application number	US-202017631928-A
Country	US
Kind code	A1
Filing date	Jul 29, 2020
Priority date	Aug 2, 2019
Publication date	Aug 11, 2022
Grant date	—

Abstract

A biological component treatment system is equipped with a biological component kit having a path through which a culture medium is capable of flowing, and a biological component treatment device that controls a flowing state of the culture medium. In a cell culturing method, there are performed a measurement step of performing a measurement of information in relation to lactic acid, a cell number calculation step of calculating a number of cells during culturing of cells on the basis of the obtained information in relation to lactic acid and calibration information, and an operation control step of controlling supply of the culture medium to the cells on the basis of the number of cells.

First claim

What is claimed is:

1 . A biological component treatment system, comprising:
a biological component kit having a path through which a culture medium for culturing cells is allowed to flow; and a biological component treatment device in which the biological component kit is set, and which is configured to control a flowing state of the culture medium; wherein the biological component treatment device comprises:
a measurement unit configured to perform a measurement of information in relation to lactic acid contained in the culture medium, on the biological component kit; and
a control unit configured to calculate a number of cells during culturing of the cells, based on the information in relation to lactic acid obtained by the measurement of the measurement unit, and calibration information that is stored in advance, and further configured to control supply of the culture medium to the cells based on the number of cells.

2 . The biological component treatment system according to claim 1 , wherein the control unit calculates an amount of lactic acid based on the information in relation to lactic acid, and derives the number of cells corresponding to the amount of lactic acid from the calibration information.

3 . The biological component treatment system according to claim 2 , wherein:
the biological component kit comprises a treatment unit configured to culture the cells, and a waste liquid route configured to cause the culture medium to flow out from the treatment unit; and the control unit corrects the amount of lactic acid by calculating an outflowing amount of the lactic acid that flows out together with the culture medium in the waste liquid route.

4 . The biological component treatment system according to claim 3 , wherein:
the biological component kit comprises a circulation circuit in which the culture medium is circulated between the biological component kit and the treatment unit, and the waste liquid route through which the culture medium is caused to flow out from the circulation circuit; and the control unit calculates the outflowing amount of the lactic acid that flows out from the circulation circuit.

5 . The biological component treatment system according to claim 1 , wherein the calibration information is set beforehand based on an input operation of an operator to the biological component treatment device before carrying out culturing of the cells.

6 . The biological component treatment system according to claim 1 , wherein:
the biological component treatment device comprises a glucose measurement unit configured to perform a measurement of information in relation to glucose contained in the culture medium, on the biological component kit; and the control unit calculates an amount of glucose based on the information in relation to glucose obtained by the measurement of the glucose measurement unit, and controls supply of the culture medium to the cells based on the amount of glucose.

7 . The biological component treatment system according to claim 6 , wherein in a case that the amount of glucose is greater than or equal to an upper limit threshold value, the control unit decreases a target supply amount of the culture medium regardless of the number of cells, whereas in a case that the amount of glucose is less than or equal to a lower limit threshold value, the control unit increases the target supply amount of the culture medium regardless of the number of cells.

8 . The biological component treatment system according to claim 1 , wherein:
the biological component kit comprises a biological component cassette that is disposed between the treatment unit and a medical bag in which the culture medium is accommodated, and is set in the biological component treatment device; the biological component cassette includes a flow path through which the culture medium flows, and further includes, in an intermediate location of the flow path, a lactic acid detection part configured to react with the lactic acid contained in the culture medium; and in a set state of the biological component cassette, the measurement unit is disposed so as to face the lactic acid detection part, and performs a measurement on the lactic acid detection part.

9 . A biological component treatment device in which a biological component kit is set, the biological component kit having a path through which a culture medium for culturing cells is allowed to flow, the biological component treatment device being configured to control a flowing state of the culture medium;
wherein the biological component treatment device comprises:
a measurement unit configured to perform a measurement of information in relation to lactic acid contained in the culture medium, on the biological component kit; and
a control unit configured to calculate a number of cells during culturing of the cells, based on the information in relation to lactic acid obtained by the measurement of the measurement unit, and calibration information that is stored in advance, and further configured to control supply of the culture medium to the cells based on the number of cells.

10 . A cell culturing method performed by a biological component treatment system, comprising:
a biological component kit having a path through which a culture medium for culturing cells is allowed to flow; and a biological component treatment device in which the biological component kit is set, and which is configured to control a flowing state of the culture medium; wherein the cell culturing method comprises:
a measurement step of performing, by a measurement unit of the biological component treatment device, a measurement of information in relation to lactic acid contained in the culture medium, on the biological component kit;
a cell number calculation step of calculating, by a control unit of the biological component treatment device, a number of cells during culturing of cells, based on the information in relation to lactic acid obtained by the measurement of the measurement unit, and calibration information that is stored in advance; and
an operation control step of controlling, by the control unit, supply of the culture medium to the cells based on the number of cells.

Assignees

• Terumo Corp

Inventors

• IGARASHI Masatsugu

Classifications

• C12M23/58

  Reaction vessels connected in series or in parallel (combinations of bioreactors with other apparatus, C12M43/00) · CPC title

• C12M23/14 Primary

  Bags · CPC title

• C12M29/10

  Perfusion · CPC title

• C12M33/07

  Dosage or metering devices therefore · CPC title

• C12M41/36

  of biomass, e.g. colony counters or by turbidity measurements (electrooptical investigation of individual particles G01N15/14, flow cytometers G01N15/1404) · CPC title