Recombinant microorganism introduced with glutaric acid transporter gene and method of preparing glutaric acid using same

Patent metadata

Field	Value
Publication number	US-2022205000-A1
Application number	US-202117549757-A
Country	US
Kind code	A1
Filing date	Dec 13, 2021
Priority date	Dec 24, 2020
Publication date	Jun 30, 2022
Grant date	—

Abstract

The present invention relates to a recombinant microorganism imparted with increased ability to produce glutaric acid by further introducing a gene encoding a polypeptide having glutaric acid transporter activity into a microorganism having ability to produce glutaric acid, and to a method of preparing glutaric acid using the recombinant microorganism. According to the present invention, glutaric acid used for the preparation of various compounds such as polyamide, polyurethane, 1,5-pentanediol, and 5-hydroxyvaleric acid can be biosynthesized at high yield.

First claim

1 . A recombinant microorganism having increased ability to produce glutaric acid, in which a gene encoding a polypeptide having glutaric acid transporter activity is introduced into a microorganism having ability to produce glutaric acid.

2 . The recombinant microorganism according to claim 1 , wherein the gene encoding the polypeptide having glutaric acid transporter activity is a gene selected from the group consisting of a ynfM gene, a yjjP gene, a yjjB gene, a yeeA gene, and a sucE1 gene.

3 . The recombinant microorganism according to claim 2 , wherein the ynfM gene is a gene encoding a polypeptide represented by SEQ ID NO: 1.

4 . The recombinant microorganism according to claim 3 , wherein the ynfM gene is represented by SEQ ID NO: 2.

5 . The recombinant microorganism according to claim 1 , wherein the microorganism having ability to produce glutaric acid is a microorganism having increased ability to produce lysine.

6 . The recombinant microorganism according to claim 1 , wherein the microorganism having ability to produce glutaric acid comprises a gene selected from the group consisting of a davA gene, a davB gene, a gabT gene, and a gabD gene.

7 . The recombinant microorganism according to claim 1 , wherein the recombinant microorganism is selected from the group consisting of Escherichia coli, Bacillus subtilis, Corynebacterium glutamicum, Lactobacillus brevis, Lactobacillus casei, Lactobacillus reuteri, Lactococcus lactis, Aspergillus niger, Saccharomyces cerevisiae , and Saccharomyces pombe.

8 . A method of preparing glutaric acid, comprising:
(a) producing glutaric acid by culturing the recombinant microorganism according to claim 1 ; and (b) recovering the produced glutaric acid.

Assignees

• Korea Advanced Inst Sci & Tech

Inventors

• Lee Sang Yup
• Han Tae Hee

Classifications

• C12P7/44 Primary

  Polycarboxylic acids · CPC title

• C12N15/77 Primary

  for Corynebacterium; for Brevibacterium · CPC title

• C12N9/00 Primary

  Enzymes; Proenzymes; Compositions thereof (preparations containing enzymes for cleaning teeth A61K8/66, A61Q11/00; medicinal preparations containing enzymes or proenzymes A61K38/43; enzyme containing detergent compositions C11D; {enzymes with nucleic acid structure, e.g. ribozymes, C12N15/113}); Processes for preparing, activating, inhibiting, separating or purifying enzymes (preparation of malt C12C1/00) · CPC title

• C12Y206/01048

  5-Aminovalerate transaminase (2.6.1.48) · CPC title

• C12Y102/01024

  Succinate-semialdehyde dehydrogenase (NAD+) (1.2.1.24) · CPC title