Integrated biochemical sensor and method of manufacturing the same

Patent metadata

Field	Value
Publication number	US-2022178908-A1
Application number	US-202117212248-A
Country	US
Kind code	A1
Filing date	Mar 25, 2021
Priority date	Dec 9, 2020
Publication date	Jun 9, 2022
Grant date	—

Abstract

Disclosed is an integrated biochemical sensor including a reference electrode, a plurality of working electrodes each having different artificial lipid membranes, and partition layers for electrically insulating the reference electrode and each of the working electrodes.

First claim

What is claimed is:

1 . An integrated biochemical sensor, comprising:
a reference electrode; a plurality of working electrodes each having different artificial lipid membranes; and partition layers for electrically insulating the reference electrode and each of the working electrodes.

2 . The integrated biochemical sensor according to claim 1 , wherein membrane potentials of the artificial lipid membranes change due to chemical reaction with biochemical ions corresponding to the artificial lipid membranes.

3 . The integrated biochemical sensor according to claim 2 , wherein the biochemical ions are detected based on a potential difference between the reference electrode and a working electrode having the artificial lipid membrane, a membrane potential of which is changed, among the working electrodes.

4 . The integrated biochemical sensor according to claim 1 , wherein each of the working electrodes further comprises a working electrode layer, a stabilizing layer, and an electrolyte layer for improving electrical connectivity between the working electrode layer and the artificial lipid membrane.

5 . The integrated biochemical sensor according to claim 4 , wherein the stabilizing layer comprises at least one of silver (Ag), silver chloride (AgCl), and polyvinyl butyral (PVB).

6 . The integrated biochemical sensor according to claim 4 , wherein the electrolyte layer comprises at least one of poly(2-hydroxyethyl methacrylate) (pHEMA) and PEDOT:PSS.

7 . The integrated biochemical sensor according to claim 1 , wherein the partition layers comprise a lower encapsulation layer for protecting the working electrodes from external impact, an insulating encapsulation layer for electrically insulating each of the working electrodes and the reference electrode, and an upper encapsulation layer for protecting the reference electrode from external impact.

8 . The integrated biochemical sensor according to claim 1 , wherein the working electrodes comprise a first working electrode for detecting biochemical ions corresponding to saltiness through a first artificial lipid membrane, a second working electrode for detecting biochemical ions corresponding to bitterness through a second artificial lipid membrane, a third working electrode for detecting biochemical ions corresponding to sweetness through a third artificial lipid membrane, and a fourth working electrode for detecting biochemical ions corresponding to sourness through a fourth artificial lipid membrane.

9 . The integrated biochemical sensor according to claim 8 , wherein the first artificial lipid membrane comprises at least one of tetradodecylammonium, bromide 1-hexadecanol, and di-n-octyl phenylphosphonate.

10 . The integrated biochemical sensor according to claim 8 , wherein the second artificial lipid membrane comprises at least one of methyl trioctyl ammonium chloride and di-n-octyl phenylphosphonate.

11 . The integrated biochemical sensor according to claim 8 , wherein the third artificial lipid membrane comprises at least one of tetradodecylammonium bromide and di-n-octyl phenylphosphonate.

12 . The integrated biochemical sensor according to claim 8 , wherein the fourth artificial lipid membrane comprises at least one of methyl trioctyl ammonium chloride, oleic acid, bis(2-ethylhexyl) phosphate, and dioctyl phenyl phosphonate.

13 . A method of manufacturing an integrated biochemical sensor, comprising:
forming a plurality of working electrode layers; forming an insulating encapsulation layer for electrically insulating each of the working electrode layers and a reference electrode layer; forming the reference electrode layer on the insulating encapsulation layer; forming an upper encapsulation layer for protecting the reference electrode layer from external impact on the insulating encapsulation layer; and forming artificial lipid membranes on the working electrode layers.

14 . The method according to claim 13 , wherein the forming of the artificial lipid membranes further comprises forming a stabilizing layer on the working electrode layers and the reference electrode layer;
forming an electrolyte layer on the working electrode layers on which the stabilizing layer has been formed and the reference electrode layer on which the stabilizing layer has been formed; and forming the artificial lipid membranes on the working electrode layers on which the electrolyte layer has been formed.

Assignees

• Daegu Gyeongbuk Inst Science & Tech

Inventors

• Jang Kyung In
• Yea Jun Woo
• Jung Han Hee

Classifications

• G01N27/327 Primary

  Biochemical electrodes {, e.g. electrical or mechanical details for in vitro measurements} · CPC title

• G01N33/483

  Physical analysis of biological material · CPC title

• G01N27/301

  Reference electrodes · CPC title

• G01N27/27

  Association of two or more measuring systems or cells, each measuring a different parameter, where the measurement results may be either used independently, the systems or cells being physically associated, or combined to produce a value for a further parameter · CPC title

• G01N27/31

  Half-cells with permeable membranes, e.g. semi-porous or perm-selective membranes · CPC title