Biological sample analysis device and biological sample analysis method

Patent metadata

Field	Value
Publication number	US-2022178832-A1
Application number	US-202017595578-A
Country	US
Kind code	A1
Filing date	May 15, 2020
Priority date	Jul 2, 2019
Publication date	Jun 9, 2022
Grant date	—

Abstract

The present invention shortens measurement time of a sample and improves measurement accuracy. The present invention is a biological sample analysis device that stores a sample containing a biological substance and a luminescent reagent in a container, detects luminescence generated by reacting the sample and the luminescent reagent, and analyzes the biological substance, the present invention including a photodetector that detects the luminescence and outputs a light intensity signal, and a calculator that subtracts the light intensity signal obtained before the sample and the luminescent reagent react from the light intensity signal obtained after the sample and the luminescent reagent react to remove light stored in the container, and calculates a value related to an amount of the biological substance.

First claim

1 . A biological sample analysis device that stores a sample containing a biological substance and a luminescent reagent in a container, detects luminescence generated by reacting the sample and the luminescent reagent, and analyzes the biological substance, the biological sample analysis device comprising:
a photodetector that detects the luminescence and outputs a light intensity signal; and a calculator that subtracts the light intensity signal obtained before the sample and the luminescent reagent react from the light intensity signal obtained after the sample and the luminescent reagent react to remove light stored in the container, and calculates a value related to an amount of the biological substance.

2 . The biological sample analysis device according to claim 1 , sequentially measuring luminescence of the sample stored in a plurality of the containers, wherein the calculator subtracts the light intensity signal obtained before the sample and the luminescent reagent react from the light intensity signal obtained after the sample and the luminescent reagent react in each of the plurality of containers.

3 . The biological sample analysis device according to claim 1 , wherein
the biological substance includes adenosine triphosphate (ATP), and ATP-derived luminescence generated by a reaction between the sample and an ATP luminescent reagent is detected.

4 . The biological sample analysis device according to claim 3 , further comprising a dispensing mechanism that dispenses a reagent into the sample, a standard solution having a known ATP amount, and a zero solution having a zero ATP amount, wherein the dispensing mechanism is controlled to equalize a mixing ratio of an ATP scavenging solution, a spore reaction solution, and an ATP extract to be added to the sample, a mixing ratio of the ATP scavenging solution, the spore reaction solution, and the ATP extract in the standard solution, and a mixing ratio of the ATP scavenging solution, the spore reaction solution, and the ATP extract in the zero solution.

5 . The biological sample analysis device according to claim 4 , wherein the dispensing mechanism is controlled to add the ATP extract after adding the ATP scavenging solution and the spore reaction solution to the standard solution.

6 . The biological sample analysis device according to claim 1 , further comprising:
a housing body that accommodates a measurement system instrument for biological sample analysis inside and includes an opening; a door that opens and closes the opening of the housing body; and uneven structures respectively provided on contact portions of the opening of the housing body and the door, the uneven structures being fitted to each other in a state where the door closes the opening.

7 . The biological sample analysis device according to claim 1 , further comprising a disposal box in which pipette tips that inject the reagent into the sample are discarded, wherein the disposal box includes disposal spaces respectively partitioned for the pipette tips to be discarded, the disposal box including a slope that inclines the pipette tips discarded in the disposal spaces in a predetermined direction.

8 . A biological sample analysis method of storing a sample containing a biological substance and a luminescent reagent in a container, detecting luminescence generated by reacting the sample and the luminescent reagent, and analyzing a biological substance, the method comprising subtracting a light intensity signal obtained before the sample and the luminescent reagent react from a light intensity signal obtained after the sample and the luminescent reagent react to remove light stored in the container, and calculating a value related to an amount of the biological substance.

Assignees

• Horiba Advanced Techno Co Ltd

Inventors

• Nakayama Hideki
• Fukao Yoshiki
• Kido Masanori
• Nakai Yoko
• Oka Yohei

Classifications

• C12M41/36

  of biomass, e.g. colony counters or by turbidity measurements (electrooptical investigation of individual particles G01N15/14, flow cytometers G01N15/1404) · CPC title

• G01N33/48

  Biological material, e.g. blood, urine (G01N33/02, G01N33/26, G01N33/44, G01N33/46 take precedence); Haemocytometers (counting blood corpuscules distributed over a surface by scanning the surface G06M11/02) · CPC title

• G01N2201/12707

  Pre-test of apparatus, e.g. dark test, sensor test · CPC title

• G01N21/77

  by observing the effect on a chemical indicator · CPC title

• G01N21/00

  Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light (G01N3/00 - G01N19/00 take precedence) · CPC title