Electroporation device and method for producing cells with introduced foreign substance

Patent metadata

Field	Value
Publication number	US-2022135937-A1
Application number	US-201917415798-A
Country	US
Kind code	A1
Filing date	Dec 19, 2019
Priority date	Dec 20, 2018
Publication date	May 5, 2022
Grant date	—

Abstract

Provided is an electroporation device and a method for producing cells with an introduced foreign substance, with which, compared to a device that performs electroporation by causing a solution (droplet) containing a foreign substance and cells to reciprocate in oil, an operation to recover a sample after a reaction is simplified.An electroporation device (1) includes a holding portion (2), a discharge generating portion (3), a conductive portion (4), and a power amount control portion (5). The holding portion (2) is configured to hold a solution containing a foreign substance and a cell. The discharge generating portion (3) includes a pair of electrodes disposed at a predetermined gap, and configured to generate an arc discharge between the pair of electrodes. The conductive portion (4) is configured to electrically connect the holding portion and the discharge generating portion, and to supply, to the holding portion, a pulsed electric current resulting from the arc discharge generated by the discharge generating portion. The power amount control portion (5) is configured to control an amount of electric energy of the pulsed electric current supplied to the holding portion.

First claim

1 . An electroporation device comprising:
a holding portion configured to hold a solution containing a foreign substance and a cell; a discharge generating portion including a pair of electrodes disposed at a predetermined gap, and configured to generate an arc discharge between the pair of electrodes; a conductive portion configured to electrically connect the holding portion and the discharge generating portion, and to supply, to the holding portion, a pulsed electric current resulting from the arc discharge generated by the discharge generating portion; and a power amount control portion configured to control an amount of electric energy of the pulsed electric current supplied to the holding portion.

2 . The electroporation device according to claim 1 , wherein
the power amount control portion includes a condenser electrically connected to a power supply portion and to the discharge generating portion, the condenser being configured to accumulate an electric charge as a result of voltage applied by the power supply portion, and the condenser being configured to discharge the accumulated electric charge to the discharge generating portion.

3 . The electroporation device according to claim 1 , wherein
the discharge generating portion is electrically connected to a power supply portion, and the power amount control portion includes a condenser electrically connected to the holding portion, the condenser being configured to accumulate the pulsed electric current supplied to the holding portion.

4 . The electroporation device according to claim 2 , further comprising:
the power supply portion configured to supply a high-voltage DC power of 3 kV or more.

5 . The electroporation device according to claim 1 , wherein
in the discharge generating portion, the predetermined gap is changeable.

6 . The electroporation device according to claim 1 , wherein
leading ends of the pair of electrodes of the discharge generating portion are formed in a hemispherical shape.

7 . The electroporation device according to claim 1 , wherein
the power amount control portion further includes a dark current control portion configured to control a dark current generated in the discharge generating portion.

8 . The electroporation device according to claim 1 , further comprising:
a cycle adjustment portion configured to cyclically apply a voltage to the discharge generating portion at a predetermined interval.

9 . The electroporation device according to claim 3 , wherein
the power amount control portion includes an inductor connected in parallel with the holding portion and the condenser.

10 . The electroporation device according to claim 9 , wherein
the power amount control portion includes a diode connected in parallel with the holding portion, to consist a forward direction of an electric current discharged from the condenser flows.

11 . A method for producing cells with an introduced foreign substance, comprising:
a holding process of causing a holding portion of the electroporation device according to claim 1 to hold a solution containing a foreign substance and a cell; a supply process of generating a pulsed electric current by arc discharge and supplying the generated pulsed electric current to the holding portion; and a recovery process of recovering, from the holding portion, the solution that has undergone the supply process.

Assignees

• National Univ Corporation Toyohashi Univ Of Technology

Inventors

• Numano Rika
• Shinozaki Ryuto

Classifications

• C12N13/00

  Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves · CPC title

• A61N1/327

  for enhancing the absorption properties of tissue, e.g. by electroporation · CPC title

• C12N15/87

  Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation · CPC title

• B01L3/502715

  characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces · CPC title

• B01L2300/0645

  Electrodes · CPC title