Methods and systems for processing polynucleotides
US-9567631-B2 · Feb 14, 2017 · US
RNA Printing and Sequencing Devices, Methods, and Systems
US2022064717A1 · US · A1
| Field | Value |
|---|---|
| Publication number | US-2022064717-A1 |
| Application number | US-202117480839-A |
| Country | US |
| Kind code | A1 |
| Filing date | Sep 21, 2021 |
| Priority date | May 26, 2015 |
| Publication date | Mar 3, 2022 |
| Grant date | — |
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Abstract
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Many important biological questions demand single-cell transcriptions on a large scale. Hence, new tools are urgently needed for efficient, inexpensive manipulation of RNA from individual cells. Described herein are devices, systems and methods for trapping single-cell lysates in sealed, microwells capable of priming RNA on glass or capturing RNA on beads.
First claim
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1 . A device or system comprising (a) one or more mRNA capture beads; (b) one or more optical barcodes; (c) a plurality of chambers, microchambers, or microwells comprising one or more mRNA capture beads and/or one or more optical barcodes; (d) wherein the plurality of chambers, microchambers, or microwells comprising one or more mRNA capture beads and/or one or more optical barcodes are configured for reversible sealing. 2 .- 31 . (canceled)
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- C12Q1/6834Primary
Enzymatic or biochemical coupling of nucleic acids to a solid phase · CPC title
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- What does patent US2022064717A1 cover?
- Many important biological questions demand single-cell transcriptions on a large scale. Hence, new tools are urgently needed for efficient, inexpensive manipulation of RNA from individual cells. Described herein are devices, systems and methods for trapping single-cell lysates in sealed, microwells capable of priming RNA on glass or capturing RNA on beads.
- Who is the assignee on this patent?
- Univ Columbia
- What technology area does this patent fall under?
- Primary CPC classification C12Q1/6834. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Thu Mar 03 2022 00:00:00 GMT+0000 (Coordinated Universal Time) (A1). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 1 related publication on this page (citations in our corpus or others sharing the same primary CPC).