Composition comprising at least one nanobomb suitable for altering a biological barrier

Patent metadata

Field	Value
Publication number	US-2022017925-A1
Application number	US-201917298884-A
Country	US
Kind code	A1
Filing date	Dec 16, 2019
Priority date	Dec 18, 2018
Publication date	Jan 20, 2022
Grant date	—

Abstract

A composition comprising at least one nanobomb comprising at least one first particle and at least one second particle in close proximity to the first particle. The at least one first particle is able to absorb electromagnetic radiation so as to generate a vapor bubble. The generation of the vapor bubble causes the at least one second particle to be propelled over a distance D. The composition is suitable to alter a biological barrier, in particular, for deforming, permeabilizing or perforating a biological barrier. A method to alter biological barriers is also disclosed.

First claim

1 . A composition comprising at least one nanobomb, said at least one nanobomb comprising n first particles and m second particles, with each of n and m being at least one, at least one of said m second particles being in close proximity to at least one of said n first particles, so as to either be in contact with or be positioned at a distance d smaller than 1 μm, said at least one n first particle being able to absorb electromagnetic radiation such so as to generate a vapor bubble, whereby said generation of said vapor bubble causes said at least one m second particle to be propelled over a distance D away from said at least one n first particle, with said distance D being at least 0.01 μm.

2 . The composition according to claim 1 , wherein said at least one m second particle of said at least one nanobomb is adapted to alter a biological barrier once propelled upon said generation of said vapor bubble.

3 . The composition of claim 1 , wherein m is larger than n.

4 . The composition of claim 1 , wherein said m second particle(s) of said at least one nanobomb has a size ranging between 10 nm and 10 μm and/or a density of at least 1 kg/dm 3 .

5 . The composition of claim 1 , wherein a majority of said n first particles comprises at least p second particles in close proximity, with p being at least 2.

6 . The composition of claim 1 , wherein said distance D ranges between 0.1 and 100 μm.

7 . The composition of claim 1 , wherein said at least one n first particle comprises a metal, a metal oxide, carbon, a carbon-based material, a light-absorbing compound or particles loaded or functionalized with one or more light-absorbing compounds or a combination thereof.

8 . The composition of claim 1 , wherein said at least one n first particle is functionalized with one or more polymer, lipid and/or molecular linker.

9 . The composition of claim 1 , wherein said m second particle(s) of said at least one nanobomb is selected from the group consisting of polymer particles, metal oxide particle, silicon or silicon oxide particles, liposomes, drug loaded polymer particles, drug loaded silicon or silicon oxide particles an drug loaded liposomes.

10 . The composition of claim 1 , wherein said at least one m second particle is functionalized with one or more charged polymer or lipid, one or more targeting moiety selected from the group consisting of antibodies, dyes, proteins, nucleic acids, drugs and/or labels and/or one or more functional group to induce a linking strategy with the at least one n first particle in close proximity.

11 . A method of altering a biological barrier, the method comprising: using the composition of claim 1 to alter at least one biological barrier.

12 . The composition of claim 1 for use in drug delivery, in intracellular delivery of compounds, in drug delivery, in cell therapy, in immunotherapy, in gene therapy and in transfection of cells.

13 . An ex vivo or in vitro method for altering a biological barrier, said method comprising:
providing the composition of claim 1 ; introducing the composition in proximity of a biological barrier; and irradiating the composition using electromagnetic radiation so as to generate vapor bubbles, thereby generating a mechanical force to propel at least part of said m second particles of said at least one nanobomb of said composition upon the generation of said vapor bubbles.

14 . The method of claim 13 , further comprising attracting said at least one nanobomb of said composition to said biological barrier by means of a magnetic field.

15 . A method of producing the composition of claim 1 , the method comprising:
providing first particles able to absorb electromagnetic radiation so as to generate a vapor bubble, providing second particles; and mixing said first particles and said second particles allowing to form at least one nanobomb, said at least one nanobomb comprising n first particles and m second particles, with each of n and m being at least one and with at least one of said m second particles being in close proximity to at least one of said n first particles, with “being in close proximity to” being defined as being either in contact with or being positioned at a distance d smaller than 1 μm.

16 . A composition comprising a nanobomb, wherein the nanobomb has n first particles and m second particles, wherein each of n and m is at least one, and wherein at least one of the m second particles is in close proximity to at least one of the n first particles, so as to either be in contact with one another or to be positioned at a distance d of less than 1 μm from one another, wherein the at least one n first particle absorbs electromagnetic radiation and thereby generates a vapor bubble, wherein generation of a vapor bubble causes said at least one m second particle to be propelled over a distance D away from said at least one n first particle, wherein distance D is at least 0.01 μm.

17 . The composition of claim 16 , wherein m is greater than n.

18 . The composition of claim 17 , wherein the at least one m second particle has a size of between 10 nm and 10 μm and/or a density of at least 1 kg/dm3.

19 . The composition of claim 18 , wherein distance D is between 0.1 and 100 μm.

20 . The composition of claim 19 , wherein the at least one n first particle comprises a metal, a metal oxide, carbon, a carbon-based material, a light-absorbing compound, a particle loaded or functionalized with one or more light-absorbing compounds, or a combination of any thereof.

Assignees

• Univ Gent

Inventors

• Braeckmans Kevin
• Fraire Juan
• De Smedt Stefaan

Classifications

• A61K47/6925

  the form being a microcapsule, nanocapsule, microbubble or nanobubble · CPC title

• A61K41/0042

  Photocleavage of drugs in vivo, e.g. cleavage of photolabile linkers in vivo by UV radiation for releasing the pharmacologically-active agent from the administered agent; photothrombosis or photoocclusion · CPC title

• A61K47/6915

  the form being a liposome with polymerisable or polymerized bilayer-forming substances, e.g. polymersomes · CPC title

• A61K48/0041

  the non-active part being polymeric · CPC title

• A61K48/0083

  characterised by an aspect of the administration regime · CPC title