Detection of components

Patent metadata

Field	Value
Publication number	US-2022016627-A1
Application number	US-201917414856-A
Country	US
Kind code	A1
Filing date	Dec 20, 2019
Priority date	Dec 20, 2018
Publication date	Jan 20, 2022
Grant date	—

Abstract

A flow apparatus for detecting a component on a surface is provided. The flow apparatus, comprising an inlet for receiving a solution of the components to be detected; a detection chamber in fluid connection with and downstream from the inlet, and in fluid connection with a downstream outlet, wherein the internal surface of the detection chamber comprises a plurality of detection zones and the detection zones are configured to adhere to the component to be detected such that the component is immobilised in the detection zones; a detector for detecting components immobilised on each of the detection zones; and a director for directing the flow of the solution of the components to each of the detection zones in sequence, wherein the director is provided by flow rates.

First claim

1 . A flow apparatus for detecting a component on a surface, comprising:
an inlet for receiving a solution of the components to be detected; a detection chamber in fluid connection with and downstream from the inlet, and in fluid connection with a downstream outlet, wherein the internal surface of the detection chamber comprises a plurality of detection zones and the detection zones are configured to adhere to the component to be detected such that the component is immobilised in the detection zones; a detector for detecting components immobilised on each of the detection zones; and a director for directing the flow of the solution of the components to each of the detection zones in sequence, wherein the director is provided by flow rates.

2 . The flow apparatus of claim 1 wherein each of the plurality of detection zones has a width of from 20 μm to 150 μm as measured in the direction from a side wall of the detection chamber to an opposing side wall of the detection chamber.

3 . The flow apparatus of claim 1 , wherein the director is provided by electroosmotic force changes.

4 . The flow apparatus of claim 1 , wherein the director is provided by pressure changes.

5 . The flow apparatus of claim 1 , wherein the director is provided by flow rate changes.

6 . The flow rate apparatus of claim 1 , wherein the director comprises two directing inlets and the two directing inlets are positioned on opposite sides of the inlet and each of the two directing inlets is configured to provide variable flow rates.

7 . The flow apparatus of claim 1 , wherein the detector is an optical detector.

8 . The flow apparatus claim 1 , wherein the optical detector detects components by interferometric scattering microscopy or total internal reflection fluorescence microscopy.

9 . The flow apparatus claim 1 , further having a treatment device in fluid connection with and upstream from the inlet.

10 . The flow apparatus of claim 9 , wherein the treatment device is a separation device.

11 . The flow apparatus of claim 10 , wherein the separation device is a batch separation device, such as a capillary electrophoresis device.

12 . The flow apparatus of claim 10 , wherein the separation device is a continuous separation device, such as a diffusion separation device or an electrophoresis separation device.

13 . The flow apparatus of claim 9 , wherein the treatment device is for adjustment of the pH and/or salt content of the solution.

14 . The flow apparatus of claim 1 , wherein the apparatus is adapted for detecting nanoparticles, protein molecules, colloids, complexes, oligomers, antibodies, nucleotides, biomolecules or biomolecular complexes.

15 . Use of the flow apparatus of claim 1 to detect a component.

16 . A method of detecting a component on a surface using a flow apparatus of claim 1 , comprising providing a solution of the components to the inlet of the flow apparatus, establishing a flow of the solution containing the components through the inlet to the detection module or detection chamber, permitting the immobilisation of the component in the detection zone for detection by the detector and detecting the component using the detector.

Assignees

• Fluidic Analytics Limilted
• Cambridge Entpr Ltd

Inventors

• Knowles Tuomas Pertti Jonathan
• Saar Kadi Liis
• Peter Quentin Alexia Eric
• Challa Pavan Kumar
• Mueller Thomas

Classifications

• B01L2400/0418

  electro-osmotic flow [EOF] · CPC title

• B01L2300/0896

  Nanoscaled · CPC title

• B01L2300/0864

  comprising only one inlet and multiple receiving wells, e.g. for separation, splitting · CPC title

• B01L3/502746

  characterised by the means for controlling flow resistance, e.g. flow controllers, baffles or throttle valves · CPC title

• B01L3/50273 Primary

  characterised by the means or forces applied to move the fluids · CPC title