Recombinant-structure protein multifilament and method for manufacturing same

Patent metadata

Field	Value
Publication number	US-2022010460-A1
Application number	US-202017427895-A
Country	US
Kind code	A1
Filing date	Feb 7, 2020
Priority date	Feb 7, 2019
Publication date	Jan 13, 2022
Grant date	—

Abstract

An object of the present invention is to provide a method for producing a modified fibroin multifilament excellent in productivity and a multifilament produced by the same. The multifilament of the present invention contains modified fibroin, in which the multifilament has 100 or more constituent single yarns, a coefficient of variation in elastic modulus is 15% or less, a coefficient of variation in strength is 15% or less, and a coefficient of variation in elongation is less than 33%.

First claim

1 . A multifilament comprising modified fibroin, wherein the multifilament has 100 or more constituent single yarns, a coefficient of variation in elastic modulus is 15% or less, a coefficient of variation in strength is 15% or less, and a coefficient of variation in elongation is less than 33%.

2 . The multifilament according to claim 1 , wherein the coefficient of variation in elongation of the multifilament is 20% or less.

3 . The multifilament according to claim 1 , wherein the coefficient of variation in elongation of the multifilament is 10% or less.

4 . The multifilament according to claim 1 , wherein a coefficient of variation in fineness of the multifilament is 20% or less.

5 . The multifilament according to claim 1 , wherein the coefficient of variation in strength of the multifilament is 10% or less, and the coefficient of variation in elastic modulus of the multifilament is 10% or less.

6 . The multifilament according to any one of claim 1 , wherein an average hydropathy index of the modified fibroin is more than −0.8.

7 . The multifilament according to claim 1 , wherein the modified fibroin is modified spider silk fibroin.

8 . The multifilament according to claim 1 to 7 , wherein the multifilament has a shrinkage history of being irreversibly shrunk after spinning.

9 . The multifilament according to claim 8 , wherein the shrinkage history is a shrinkage history of being irreversibly shrunk by bringing the multifilament into contact with water or a shrinkage history of being irreversibly shrunk by heating and relaxing the multifilament.

10 . The multifilament according to claim 8 , wherein a shrinkage rate of the multifilament having a shrinkage history of being irreversibly shrunk after spinning is 5% or less, the shrinkage rate being defined by the following equation:
Shrinkage rate [%]=(1−(length of multifilament when dried from wet state/length of multifilament when in wet state))×100.

11 . The multifilament according to claim 1 , wherein a coefficient of variation in fineness of the multifilament is 0.01% to 6.5%.

12 . The multifilament according to claim 1 , wherein the coefficient of variation in strength of the multifilament is 0.01% to 3.8%.

13 . A multifilament comprising a recombinant structural protein, wherein the multifilament has 100 or more constituent single yarns, and a coefficient of variation in elongation is less than 33%.

14 . A method for producing a multifilament, comprising a step of discharging a spinning raw material solution containing modified fibroin and a solvent from a spinning nozzle having 100 or more holes, and bringing the spinning raw material solution into contact with a coagulation liquid to coagulate the modified fibroin.

15 . A method for producing a multifilament, comprising a step of discharging a spinning raw material solution containing a recombinant structural protein and a solvent from a spinning nozzle having 100 or more holes, and bringing the spinning raw material solution into contact with a coagulation liquid to coagulate the recombinant structural protein.

Assignees

• Spiber Inc

Inventors

• ABE Yunosuke
• Ishii Hideto
• Sato Ryoko
• Sato Akito
• Ando Hirotada
• Kano Hiroshi
• KOTAKA Koichi
• Kobayashi Jun

Classifications

• C07K14/43518

  from spiders · CPC title

• D01D5/06 Primary

  Wet spinning methods {(D01D5/0046 takes precedence)} · CPC title

• D10B2211/22

  Fibroin · CPC title

• D01F4/02 Primary

  from fibroin · CPC title

• A61L27/227

  Other specific proteins or polypeptides not covered by A61L27/222, A61L27/225 or A61L27/24 · CPC title