System and method for data analysis in quantitative pcr measurements

Patent metadata

Field	Value
Publication number	US-2021395807-A1
Application number	US-202117350666-A
Country	US
Kind code	A1
Filing date	Jun 17, 2021
Priority date	Jun 17, 2020
Publication date	Dec 23, 2021
Grant date	—

Abstract

Embodiments of the present invention relate to a system and method for determining quantity of target nucleic acid sequence in a sample. During a PCR-based amplification reaction, fluorescence intensity signals are acquired that form an amplification profile from which an exponential amplification region is desirably identified. In determining the exponential region, embodiments of the present invention determine a fluorescence threshold by background subtraction, test the feasibility of matching a signal to a reference curve and, in the event the feasibility test is successful, determine the matching parameters that quantify the initial amplicon number, and signal detection that reduces systematic errors in the measurements and increase the sensitivity of the measurement by decreasing the apparent noise-floor.

First claim

What is claimed is:

1 . A method for determining a quantity of a target nucleic acid in a sample, the method comprising:
performing a first amplification reaction on a plurality of control samples; receiving a plurality of first optical signals as a function of a first cycle number for the plurality of the control samples, wherein the plurality of the first optical signals comprises a plurality of first background signals; performing a second amplification reaction on the sample; receiving a plurality of second optical signals as a function of a second cycle number for the target nucleic acid in the sample, wherein the plurality of the second optical signals comprise at least one of the plurality of the first background signals and a plurality of second background signals; optimizing, using a processor, the plurality of the first background signals from the plurality of the first optical signals for each of the plurality of the control samples, wherein the optimizing the plurality of the first background signals comprises subtracting the plurality of the first background signals from the plurality of the second optical signals received to obtain a mean baseline, wherein the mean baseline corresponds to the second cycle number such that the plurality of the second optical signals corresponds to a predetermined value; validating, using the processor, each of the plurality of the second optical signals against at least one of a plurality of reference amplification curves; and computing, using the processor, the quantity of the target nucleic acid in the sample from the validated plurality of the second optical signals obtained as the function of the second cycle number for the target nucleic acid in the sample, wherein computing the quantity of the target nucleic acid in the sample from the validated plurality of the second optical signals comprises performing an affine transformation to apply at least one of a linear transformation and a translation on the validated plurality of the second optical signals.

2 . The method of claim 1 , wherein the receiving the plurality of the first optical signals as the function of the first cycle number comprises:
detecting the plurality of the first optical signals for each of the plurality of the control samples at each cycle of the first amplification reaction, wherein each cycle of the first amplification reaction corresponds to the first cycle number; and plotting the plurality of the first optical signals detected as the function of the first cycle number for each of the plurality of the control samples to obtain a first amplification curve, wherein the first amplification curve represents a ratio of each of the plurality of the first optical signals to a passive reporter dye optical signal as the function of the first cycle number.

3 . The method of claim 1 , wherein the receiving the plurality of the second optical signals as the function of the second cycle number comprises:
detecting the plurality of the second optical signals for the target nucleic acid in the sample at each cycle of the second amplification reaction, wherein each cycle of the second amplification reaction corresponds to the second cycle number; and plotting the plurality of the second optical signals detected as the function of the second cycle number for the target nucleic acid in the sample to obtain a second amplification curve, wherein the second amplification curve represents a ratio of each of the plurality of the second optical signals to a passive reporter dye optical signal as the function of the second cycle number.

4 . The method of claim 1 , wherein the validating each of the plurality of the second optical signals against the at least one of the plurality of the reference amplification curves comprises:
generating at least the one of the plurality of the reference amplification curves, wherein the at least one of the plurality of the reference amplification curves comprises a plurality of third optical signals corresponding to a reference sample, wherein the at least one of the plurality of the reference amplification curves comprises the plurality of the third optical signals corresponding to a background region, an exponential growth region, and a plateau region; projecting each of the plurality of the second optical signals on to the at least one of the plurality of the reference amplification signals, wherein the projecting each of the plurality of the second optical signals on to the at least one of the plurality of the reference amplification curves comprises determining whether each of the plurality of the second optical signals obtained as the function of the second cycle number for the target nucleic acid in the sample collapses on to the at least one of the plurality of the reference amplification curves; and determining a threshold value for the plurality of the second optical signals projected on to the at least one of the plurality of the reference amplification curves.

5 . The method of claim 4 , further comprising plotting the plurality of the second optical signals projected on to the at least one of the plurality of the reference amplification curves to obtain a validated reference amplification curve.

6 . The method of claim 1 , further comprising detecting the plurality of third optical signals for a plurality of amplicons, wherein each of the plurality of the third optical signals correspond to an optical signal from at least one fluorescent dye.

7 . The method of claim 1 , wherein the linear transformation comprises scaling, and wherein the translation is selected from a group comprising a horizontal shift and a vertical scaling.

8 . The method of claim 1 , wherein the control sample is an extraction blank.

9 . The method of claim 1 , wherein the control sample is a non-template control.

10 . The method of claim 1 , wherein the validating each of the plurality of second optical signals against at least one of a plurality of reference amplification curves comprises:
generating the plurality of the reference amplification curves, wherein each of the plurality of the reference amplification curves comprises a plurality of third optical signals corresponding to a reference sample, wherein each of the plurality of the reference amplification curves comprises the plurality of the third optical signals corresponding to a background region, an exponential growth region, and a plateau region; projecting the plurality of the second optical signals onto the plurality of the reference amplification curves, wherein projecting the plurality of the second optical signals onto the plurality of the reference amplification curves comprises determining whether each of the plurality of the second optical signal obtained as the function of the second cycle number for the target nucleic acid in the sample collapses on to at least one of the plurality of reference amplification curves; and determining a threshold value for the plurality of the second optical signals projected on to the at least one of the plurality of the reference amplification curves.

11 . The method of claim 1 , wherein the sample is a pre-amplified DNA sample.

12 . The method of claim 1 , wherein the sample is a pre-amplified RNA sample.

13 . The method of claim 1 , further comprising normalizing the plurality of the first optical signals and the plurality of the second optical signals, wherein normalizing the plurality of the first and the second optical signals comprises determining a ratio of each of the plurality of the first and the second optical signals to a passive reporter dye optical signal.

14 . A

Assignees

• Government Of The Us Secretary Of Commerce

Inventors

• Patrone Paul Nathan
• Kearsley Anthony Jose
• Romsos Erica Lee
• Vallone Peter Michael

Classifications

• C12Q1/6851 Primary

  Quantitative amplification · CPC title

• C12Q1/6844 Primary

  Nucleic acid amplification reactions · CPC title

• C12Q1/6806

  Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay (C12Q1/6804 takes precedence) · CPC title

• G16B40/10

  Signal processing, e.g. from mass spectrometry [MS] or from PCR · CPC title

• G02B2207/113

  Fluorescence · CPC title