Methods and systems for in vivo full-field interference microscopy imaging

Patent metadata

Field	Value
Publication number	US-2021345873-A1
Application number	US-201917280646-A
Country	US
Kind code	A1
Filing date	Sep 27, 2019
Priority date	Sep 28, 2018
Publication date	Nov 11, 2021
Grant date	—

Abstract

According to one aspect, the invention relates to a system (101) for in vivo, full-field interference microscopy imaging of a scattering three-dimensional sample. It comprises a full-field OCT imaging system (130) for providing en face images of the sample, wherein said full-field OCT system comprises an interference device (145) with an object arm (147) intended to receive the sample and a reference arm (146) comprising an optical lens (134) and a first reflection surface (133), and an acquisition device (138) configured to acquire a temporal succession of two-dimensional interferometric signals (I1, I2) resulting from interferences produced at each point of an imaging field; an OCT imaging system (110) for providing at the same times of acquisition of said two-dimensional interferometric signals, cross-sectional images of both the sample and a first reflection surface (133) of said full-field OCT imaging system (130); a processing unit (160) configured to determine a plurality of en face images (X-Y) of a plurality of slices of the sample, each en face image being determined from at least two two-dimensional interferometric signals (I1, I2) having a given phase shift; determine from the cross-sectional images provided by the OCT imaging system (110) at the times of acquisition of each of said two two-dimensional interferometric signals (I1, I2) a depth (z) for each en face image (X-Y) of said plurality of slices; determine a 3D image of the sample from said plurality of en face images of said plurality of slices of the sample and depths.

First claim

1 . A method for in vivo, full-field interference microscopy imaging of a scattering three-dimensional sample, comprising:
disposing the sample in an object arm of an interference device of a full-field OCT imaging system, wherein said interference device further comprises a reference arm with an optical lens and a first reflection surface; producing, at each point of an imaging field, an interference between a reference wave obtained by reflection of incident light waves on an elementary surface of the first reflection surface corresponding to said point of the imaging field and an object wave obtained by backscattering of incident light waves by a voxel of a slice of the sample at a given depth, said voxel corresponding to said point of the imaging field, acquiring, using an acquisition device of said full-field OCT imaging system, a temporal succession of two-dimensional interferometric signals resulting from the interferences produced at each point of the imaging field; storing, for each two-dimensional interferometric signal, a time of acquisition; providing, at each time of acquisition of the two-dimensional interferometric signals, cross-sectional images (X-Z) of both the sample and said first reflection surface of said full-field OCT imaging system using an OCT imaging system; determining a plurality of en face images (X-Y) of a plurality of slices of the sample, each en face image being determined from at least two two-dimensional interferometric signals having a given phase shift; determining from the cross-sectional images provided by the OCT imaging system at the times of acquisition of each of said two two-dimensional interferometric signals a depth (z) for each en face image (X-Y) of said plurality of slices; determining a 3D image of the sample from said plurality of en face images of said plurality of slices of the sample and depths.

2 . The method according to claim 1 , wherein said full-field OCT imaging system and said OCT imaging system being mounted on a moving platform, the method further comprises moving said platform at least along an optical axis (Z) of the object arm to determine said plurality of en face images (X-Y).

3 . The method according to claim 1 , further comprising moving said platform at least along a direction (X, Y) perpendicular to said optical axis of the object arm.

4 . The method according to claim 2 , wherein said reference arm being mounted on a moving platform, the method further comprises moving said platform to compensate for defocus.

5 . The method according to claim 1 , wherein said object arm being mounted on a moving platform, the method further comprises moving said platform along an optical axis (Z) of the object arm to determine said plurality of en face images (X-Y).

6 . The method according to claim 5 , wherein said reference arm being mounted on a moving platform, the method further comprises moving said platform to compensate for defocus.

7 . The method according to claim 1 , wherein said reference arm being mounted on a moving platform, the method further comprises moving said platform along an optical axis (X) of the reference arm to compensate for defocus, to determine said plurality of en face images (X-Y).

8 . The method according to claim 1 , further comprising position shifting said first reflection surface of the reference arm of the full-field OCT imaging system to provide said phase shift between said at least two two-dimensional interferometric signals.

9 . The method according to claim 1 , further comprising selecting in said temporal succession of two-dimensional interferometric signals acquired by the acquisition device, said at least two two-dimensional interferometric signals having said phase shift, wherein the phase shift results from in vivo movements of the sample.

10 . A system for in vivo, full-field interference microscopy imaging of a scattering three-dimensional sample comprising:
a full-field OCT imaging system for providing en face images of the sample, wherein said full-field OCT system comprises:
an interference device comprising an object arm intended to receive the sample and a reference arm comprising an optical lens and a first reflection surface, wherein said object arm and said reference arm are separated by a beam splitter and wherein the interference device is adapted to produce, when the sample is disposed on the object arm of the interference device, at each point of an imaging field, an interference between a reference wave obtained by reflection of incident light waves on an elementary surface of the first reflection surface corresponding to said point of the imaging field and an object wave obtained by backscattering of incident light waves by a voxel of a slice of the sample at a given depth, said voxel corresponding to said point of the imaging field,
an acquisition device configured to acquire a temporal succession of two-dimensional interferometric signals resulting from the interferences produced at each point of the imaging field,
an OCT imaging system for providing at the same times of acquisition of said two-dimensional interferometric signals, cross-sectional images of both the sample and said first reflection surface of said full-field OCT imaging system; a processing unit configured to:
determine a plurality of en face images (X-Y) of a plurality of slices of the sample, each en face image being determined from at least two two-dimensional interferometric signals having a given phase shift;
determine from the cross-sectional images provided by the OCT imaging system at the times of acquisition of each of said two two-dimensional interferometric signals a depth (z) for each en face image (X-Y) of said plurality of slices;
determine a 3D image of the sample from said plurality of en face images of said plurality of slices of the sample and depths.

11 . The system according to claim 10 , wherein said first reflection surface of the reference arm of the full-field OCT imaging system is position shifted to provide said optical path difference between said at least two-dimensional interferometric signals.

12 . The system according to claim 10 , wherein the processing unit is further configured to:
select in said temporal succession of two-dimensional interferometric signals acquired by the acquisition device, said at least two-dimensional interferometric signals having said given optical path difference, wherein the optical path difference results from in vivo movements of the sample.

13 . The system according to claim 10 , wherein said object arm of the full-field OCT imaging system further comprises an optical lens.

14 . The system according to any of claim 10 , wherein said reference arm and/or object arm of the full-field OCT imaging system can be moved with respect to said beam splitter of the interference device of said full-field OCT imaging system.

15 . The system according to claim 10 , further comprising a moving platform, wherein said full-field OCT imaging system and said OCT imaging system are mounted on said moving platform.

16 . The system according to claim 10 , wherein the OCT imaging system is a spectral domain OCT imaging system, a time-domain OCT imaging system, or a swept-source OCT imaging system.

Assignees

• Paris Sciences Lettres Quartier Latin
• Centre Nat Rech Scient
• Ecole Superieure Physique & Chimie Ind Ville De Paris

Inventors

• Mazlin Viacheslav
• Xiao Peng
• Fink Mathias
• Boccara Albert Claude

Classifications

• G01B9/02091

  Tomographic interferometers, e.g. based on optical coherence · CPC title

• A61B3/102 Primary

  for optical coherence tomography [OCT] · CPC title

• G01B9/02077

  of the object · CPC title

• A61B3/13

  Ophthalmic microscopes · CPC title