Systems and Methods for Determining Probative Samples and Isolation and Quantitation of Cells

Patent metadata

Field	Value
Publication number	US-2021339253-A1
Application number	US-202117369490-A
Country	US
Kind code	A1
Filing date	Jul 7, 2021
Priority date	Sep 30, 2014
Publication date	Nov 4, 2021
Grant date	—

Abstract

Embodiments of the present disclosure relate to a magnetic bead platform for isolating sperm cells from biological samples. In some embodiments, such magnetic bead platforms integrate recognition reagents to its surface to bind target cells, such as sperm cells. Such embodiments provide the ability to at least one of rapidly isolate and quantitate sperm cells from biological samples as occur in sexual assault evidence, for example, thereby enhancing identification of suspects in these cases and contributing to the safety of society.

First claim

What is claimed is:

1 . A system for detecting and isolating target cells in a biological sample, comprising:
a biological sample receptacle configured to receive a biological sample, the biological sample receptacle containing one or more magnetic beads, wherein the magnetic beads comprise at least one outer surface, further wherein the biological sample receptacle comprises at least one outer surface and at least one inner surface; a recognition reagent linked to the outer surface of the one or more magnetic beads, the recognition reagent configured to capture one or more target cells contained in the biological sample by binding with the one or more target cells; and a magnetic source configured to apply an external magnetic field to the outer surface of the biological sample receptacle thereby immobilizing the one or more magnetic beads to the at least one inner surface of the biological sample receptacle.

2 . The system of claim 1 , wherein the biological sample receptacle is selected from the group consisting of a well, cuvette, tube, vial, capillary, assay strip, and combinations thereof.

3 . The system of claim 1 , wherein the biological sample receptacle is a microfluidic device.

4 . The system of claim 1 , wherein the one or more target cells are sperm cells.

5 . The system of claim 1 , wherein the recognition reagent comprises a saccharide sequence.

6 . The system of claim 6 , wherein the saccharide sequence comprises an oligosaccharide sequence.

7 . The system of claim 7 , wherein the oligosaccharide sequence comprises a sialyl-Lewis x oligosaccharide sequence.

8 . A method for detecting and isolating target cells in a biological sample, comprising:
providing a biological sample receptacle, the biological sample receptacle having at least one outer surface and at least one inner surface, wherein the biological sample receptacle is configured to accept the biological sample, the biological sample comprising one or more target cells; transferring one or more magnetic beads having at least one outer surface, into contact with the at least one inner surface of the biological sample receptacle, the at least one outer surface of the one or more magnetic beads being linked to a recognition reagent, wherein the recognition reagent is configured to capture the one or more target cells; exposing the biological sample to the at least one outer surface of the one or more magnetic beads; capturing the one or more target cells contained in the biological sample, wherein the one or more target cells are captured by binding with the recognition reagent; and applying an external magnetic source to the at least one outer surface of the biological sample receptacle to immobilize the one or more magnetic beads to the at least one inner surface of the biological sample receptacle.

9 . The method of claim 9 , wherein the biological sample receptacle is selected from the group consisting of a well, cuvette, tube, vial, capillary, assay strip, and combinations thereof.

10 . The method of claim 9 , wherein the biological sample receptacle is a microfluidic device.

11 . The method of claim 9 , wherein the one or more target cells are sperm cells.

12 . The method claim 9 , wherein the recognition reagent comprises a saccharide sequence.

13 . The method of claim 14 , wherein the saccharide sequence comprises an oligosaccharide sequence.

14 . The method of claim 15 , wherein the oligosaccharide sequence comprises a sialyl-Lewis x oligosaccharide sequence.

Assignees

• Dxnow Inc
• Brigham & Womens Hospital Inc
• Univ Leland Stanford Junior

Inventors

• Klevan Leonard
• Inci Fatih
• Demirci Utkan

Classifications

• C12M23/16

  Microfluidic devices; Capillary tubes (integrated microfluidic structures B01L3/5027; microreactors B01J19/0093) · CPC title

• B01L3/502761 Primary

  specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules · CPC title

• C12M41/48

  Automatic or computerized control (automatic analysis G01N35/00) · CPC title

• C12M47/04 Primary

  Cell isolation or sorting (purging biological preparations of unwanted cells C12N5/0081, determining the presence or kind of microorganism C12Q1/04) · CPC title

• C12M41/36

  of biomass, e.g. colony counters or by turbidity measurements (electrooptical investigation of individual particles G01N15/14, flow cytometers G01N15/1404) · CPC title