Integrated Microfluidic Device for Capture and Spectroscopic Characterization of Live Cells Under Various Treatment Conditions

Patent metadata

Field	Value
Publication number	US-2021302312-A1
Application number	US-201917262347-A
Country	US
Kind code	A1
Filing date	Jul 25, 2019
Priority date	Jul 25, 2018
Publication date	Sep 30, 2021
Grant date	—

Abstract

A detection unit, device, and system for cell capture, spectral analysis, and drug interaction monitoring. The detection unit includes an IR-transparent substrate connected to a plasmonic metasurface with an array of metallic antennas. In a detection device, the detection unit is connected to a microfluidic chamber with a channel such that the channel extends along the metasurface. For the detection system, the detection device is mounted on a microscope. The infrared spectra are collected in reflection, with infrared light impinging on the metasurface from the substrate side and returning back through the substrate in the form of reflected infrared light. The system includes a syringe pump for injecting live cells into the chamber. An AC source is connected to the metasurface for cell capture and its AC voltage creates a dielectrophoretic (DEP) force that causes the live cells to move from the chamber and onto the metasurface for spectral analysis.

First claim

What is claimed is:

1 . A detection unit, comprising:
an IR-transparent substrate; and a plasmonic metasurface connected to the IR-transparent substrate, the plasmonic metasurface comprising an array of metallic plasmonic wires.

2 . The detection unit of claim 1 , wherein the substrate is composed of CaF 2 .

3 . The detection unit of claim 1 , wherein the metallic plasmonic wires are gold nanoantennas.

4 . The detection unit of claim 1 , further comprising an AC source connected to the array of metallic plasmonic wires.

5 . The detection unit of claim 1 , wherein the metallic plasmonic wires are electrically-biased.

6 . The detection unit of claim 1 , wherein the metallic plasmonic wires are attached to contact pads on the plasmonic metasurface.

7 . The detection unit of claim 1 , wherein the IR-transparent substrate is configured to receive and reflect infrared light.

8 . The detection unit of claim 1 , wherein the IR-transparent substrate is within a well of a multi-well microplate.

9 . The detection unit of claim 7 , wherein the IR-transparent substrate comprises a bottom of the well.

10 . The detection unit of claim 7 , wherein the plasmonic metasurface is positioned above the IR-transparent substrate closer to an open end of the well.

11 . The detection unit of claim 9 , wherein IR transparent substrate is configured to receive and reflect infrared light at the bottom of the well.

12 . A detection device, comprising:
a unit comprising a substrate attached to a metasurface, the metasurface having a plurality of wires extending therethrough; and a microfluidic chamber with a microfluidic channel, the microfluidic chamber connected to the metasurface such that the microfluidic channel extends along the metasurface.

13 . The detection device of claim 12 , further comprising a top clamp attached to the substrate and a bottom clamp attached to the microfluidic chamber.

14 . The detection device of claim 13 , wherein the top clamp and the bottom clamp are attached via one or more connectors.

15 . The detection device of claim 14 , wherein the one or more connectors extend through the top clamp and the bottom clamp.

16 . The detection device of claim 12 , further comprising an AC source connected to the metasurface.

17 . A detection system, comprising:
a unit comprising a substrate attached to a metasurface, the metasurface having a plurality of wires extending therethrough; and a microfluidic chamber with a microfluidic channel, the microfluidic chamber connected to the metasurface such that the microfluidic channel extends along the metasurface; an AC source connected to the plurality of wires in the metasurface; and a microscope positioned above the unit, the microscope configured to emit IR light at the metasurface and collect IR light reflected from the metasurface.

18 . The detection system of claim 17 , further comprising an injection device connected to the microfluidic channel.

19 . The detection system of claim 17 , further comprising an imaging device connected to the microscope.

20 . The detection system of claim 19 , wherein the imaging device is configured to capture image data from the collected IR light reflected from the metasurface.

Assignees

• Univ Cornell

Inventors

• Shvets Gennady
• Kelp Glen
• Huang He

Classifications

• G01N21/554 Primary

  detecting the surface plasmon resonance of nanostructured metals, e.g. localised surface plasmon resonance · CPC title

• G02B27/42

  Diffraction optics {, i.e. systems including a diffractive element being designed for providing a diffractive effect}(G02B27/60 takes precedence) · CPC title

• G01N21/35

  using infrared light (G01N21/39 takes precedence) · CPC title

• B01L3/502715

  characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces · CPC title

• G02B21/06

  Means for illuminating specimens · CPC title