Systems and methods for analyzing an analyte extracted from a sample using an adsorbent material

Patent metadata

Field	Value
Publication number	US-2021302288-A1
Application number	US-202117346576-A
Country	US
Kind code	A1
Filing date	Jun 14, 2021
Priority date	Jun 3, 2016
Publication date	Sep 30, 2021
Grant date	—

Abstract

Systems and methods for analyzing an analyte extracted from a sample using an adsorbent material.

First claim

1 . A method for extracting an analyte from a sample, the method comprising:
introducing a sample into a capillary that comprises an adsorbent material that coats a portion of walls of the capillary; interacting the sample within the capillary with the adsorbent material within the capillary such that at least one analyte is extracted from the sample and binds to the adsorbent material; introducing an extraction solvent into the capillary; and interacting the extraction solvent with the adsorbent material in order to elute the at least one analyte from the adsorbent material.

2 . (canceled)

3 . The method according to claim 1 , wherein the capillary is moved a first time to cause the sample to interact with the adsorbent material and then moved a second time to cause the extraction solvent to interact with the adsorbent material after the sample has interacted with the adsorbent material.

4 . The method according to claim 3 , wherein the sample and the extraction solvent are both within the capillary and are separated from each other by an air gap.

5 - 7 . (canceled)

8 . The method according to claim 1 , wherein the sample is removed from the capillary and the extraction solvent is introduced to the capillary in a manner that the adsorbent material interacts with the extraction solvent.

9 . The method according to claim 1 , further comprising analyzing the extracted analyte.

10 . The method according to claim 9 , wherein analyzing comprises:
applying a voltage to the extraction solvent comprising the at least one analyte in the capillary so that the analyte is expelled from the capillary, thereby generating ions of the analyte; and analyzing the ions.

11 . The method according to claim 9 , wherein analyzing comprises:
removing the solvent comprising the at least one analyte from the capillary; and conducting an assay that analyzes the analyte.

12 . The method according to claim 1 , wherein the solvent is immiscible with the sample.

13 . The method according to claim 1 , wherein the solvent is miscible with the sample.

14 . The method according to claim 1 , wherein the adsorbent material comprises one or more molecules that react with the at least one target analyte.

15 . The method according to claim 14 , further comprising conducting a reaction between the one or more molecules and the at least one target analyte to produce a reaction product.

16 . The method according to claim 15 , further comprising analyzing the reaction product.

17 . The method according to claim 16 , wherein analyzing comprises:
applying a voltage to the extraction solvent comprising the reaction product in the capillary so that the reaction product is expelled from the capillary, thereby generating ions of the analyte; and analyzing the ions.

18 . (canceled)

19 . The method according to claim 1 , wherein the extraction solvent comprises one or more molecules that react with the at least one target analyte.

20 . The method according to claim 19 , further comprising conducting a reaction between the one or more molecules and the at least one target analyte to produce a reaction product.

21 . The method according to claim 20 , further comprising analyzing the reaction product.

22 . The method according to claim 21 , wherein analyzing comprises:
applying a voltage to the extraction solvent comprising the reaction product in the capillary so that the reaction product is expelled from the capillary, thereby generating ions of the analyte; and analyzing the ions.

23 . The method according to claim 21 , wherein analyzing comprises:
removing the extraction solvent comprising the reaction product from the capillary; and conducting an assay that analyzes the reaction product.

24 . The method according to claim 1 , wherein the adsorbent material includes an internal standard.

25 - 27 . (canceled)

Assignees

• Purdue Research Foundation

Inventors

• Ouyang Zheng
• Zhang Wenpeng

Classifications

• G01N27/62

  by investigating the ionisation of gases, e.g. aerosols; by investigating electric discharges, e.g. emission of cathode · CPC title

• G01N1/40 Primary

  Concentrating samples · CPC title

• G01N1/28

  Preparing specimens for investigation {including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q}(mounting specimens on microscopic slides G02B21/34; means for supporting the objects or the materials to be analysed in electron microscopes H01J37/20 {; laboratory gas handling apparatus B01L5/00}) · CPC title

• G01N1/405 Primary

  by adsorption or absorption · CPC title

• H01J49/0436

  using a membrane permeable to liquids · CPC title