Biopolymer-coated two-dimensional transition metal chalcogenides having potent antimicrobial activity

Patent metadata

Field	Value
Publication number	US-2021289791-A1
Application number	US-201917264766-A
Country	US
Kind code	A1
Filing date	Jul 30, 2019
Priority date	Jul 30, 2018
Publication date	Sep 23, 2021
Grant date	—

Abstract

Biocompatible polymer-coated transition metal chalcogenide (TMC) nanomaterials are provided herein. In particular, provided herein are two-dimensional polymer-coated TMC nanomaterials having excellent antimicrobial properties and biocompatibility, as well as methods of inhibiting microbiological growth on, or in, devices coated by or otherwise comprising the biocompatible polymer-coated transition metal chalcogenide (TMC) nanomaterials. In some cases, the biopolymer coating encapsulating the TMC nanomaterial comprises short synthetic single-stranded DNAs (ssDNAs). As described herein, ssDNA-encapsulated TMDCs exhibit no cytotoxicity against human cell lines at concentrations up to 0.25 mg/mL, but they exhibit exceptionally strong bactericidal activity against both gram-positive and gram-negative bacteria, including antibiotic-resistant Escherichia coli and a gram-positive methicillin-resistant Staphylococcus aureus (MRSA) strain. In other cases, TMDCs encapsulated by poly-L-lysine and Pluronic F77 display strong activity against multi drug resistance bacteria and form coatings that strongly inhibit bacterial biofilms, while TMDCs encapsulated by chitosan exhibit strong activity against fungi.

First claim

1 . A biocompatible polymer-coated transition metal chalcogenide (TMC) nanomaterial, comprising a two-dimensional dispersion of TMC at least partially coated with a biocompatible polymer.

2 . The biocompatible polymer-coated TMC nanomaterial of claim 1 , wherein the TMC is a transition metal dichalcogenide, transition metal monochalcogenide, transition metal trichalcogenide, post-transition metal monochalcogenide, or post-transition metal trichalcogenide.

3 . The biocompatible polymer-coated TMC nanomaterial of claim 2 , wherein the TMC is selected from MoS 2 , MoSe 2 , WS 2 , WSe 2 , Bi 2 Se 3 , Bi 2 S 3 , Bi 2 Te 3 , Sb 2 Se 3 , Sb 2 S 3 , Sb 2 Te 3 , SnSe, and SnS.

4 . The biocompatible polymer-coated TMC nanomaterial of claim 1 , wherein the biocompatible polymer is selected from a single-stranded DNA (ssDNA), a single-stranded RNA (ssRNA), peptide, poly-L-lysine, poly-D-lysine, Pluronic polymers, Tetronic polymers, and chitosan, or a combination thereof.

5 . A method for inhibiting microbiological growth on, or in, a medium which comprises contacting the medium with a biocompatible polymer-coated TMC nanomaterial according to claim 1 .

6 . The method of claim 5 , wherein contacting the medium with the biocompatible polymer-coated TMC nanomaterial inhibits growth of one or more multidrug resistant (MDR) microbial organisms.

7 . A method for preparing a biocompatible polymer-coated transition metal chalcogenides (TMC), the method comprising
(a) ultrasonicating a bulk TMDC powder in an aqueous solutions comprising a biocompatible polymer, whereby an ultrasonicated TMDC solution is obtained; (b) centrifuging the ultrasonicated TMDC solution, whereby a supernatant and a precipitate are obtained; and (c) collecting the supernatant which comprises a two-dimensional dispersion of biocompatible polymer-coated TMDC.

8 . The method of claim 7 , wherein the TMC is a transition metal dichalcogenide, transition metal monochalcogenide, transition metal trichalcogenide, post-transition metal monochalcogenide, or post-transition metal trichalcogenide.

9 . The method of claim 7 , wherein the biocompatible polymer is selected from a single-stranded DNA (ssDNA), a single-stranded RNA (ssRNA), peptide, poly-L-lysine, poly-D-lysine, Pluronic polymers, Tetronic polymers, and chitosan, or a combination thereof.

10 . The method of claim 7 , wherein the biocompatible polymer is ssDNA or ssRNA having a length of 10 nucleotides to 80 nucleotides.

11 . The method of claim 10 , wherein the ssDNA has a nucleotide sequence comprising at least ten repeats of GT (GT 10 ).

12 . The method of claim 10 , wherein the ssDNA has a nucleotide sequence comprising 10-20 consecutive thymidine (T) bases.

13 . A method for inhibiting microbiological growth on, or in, a medium which comprises coating the medium with a biocompatible polymer-coated TMC nanomaterial prepared according to claim 7 .

14 . The method of claim 13 , wherein the medium is a medical device.

15 . The method of claim 13 , wherein coating the medium with the biocompatible polymer coated TMC nanomaterial inhibits growth of one or more multidrug resistant (MDR) microbial organisms.

Assignees

• Univ Arizona State

Inventors

• Green Alexander
• Wang Qing Hua
• Saha Sanchari
• Debnath Abhishek

Classifications

• A01N59/16 Primary

  Heavy metals; Compounds thereof · CPC title

• A01N25/10

  Macromolecular compounds · CPC title

• A61L2300/252

  Polypeptides, proteins, e.g. glycoproteins, lipoproteins, cytokines · CPC title

• A01N59/02

  Sulfur; Selenium; Tellurium; Compounds thereof · CPC title

• B82Y40/00

  Manufacture or treatment of nanostructures · CPC title