Microfluidic Sequencing Device for Multiplying and Separating Molecular Chains, and Method for Separating Molecular Chains Obtained from an Amplification Reaction

Patent metadata

Field	Value
Publication number	US-2021237063-A1
Application number	US-201917051688-A
Country	US
Kind code	A1
Filing date	May 2, 2019
Priority date	May 8, 2018
Publication date	Aug 5, 2021
Grant date	—

Abstract

A microfluidic sequencing device for multiplying and separating molecular chains, and which is designed to receive biochemical material, includes at least one supply opening for supplying biochemical material into the sequencing device. The sequencing device additionally has at least one microfluidic separation unit, which has at least one separation channel with at least one amplification cavity for multiplying molecular chains supplied via the supply opening as the biochemical material and with at least one separating unit which is connected or can be connected to the amplification cavity microfluidically and includes a multi-porous material. The separating unit is designed to separate nucleic acids from other macromolecular components and/or to separate nucleic acids. Furthermore, the sequencing device has at least one discharge opening for discharging nucleic acids separated in the separation unit as biochemical material out of the sequencing device.

First claim

1 . A microfluidic sequencing device for reproducing and separating molecular chains, the sequencing device configured to accommodate biochemical material, the sequencing device comprising:
a supply opening configured for supplying biochemical material into the sequencing device; at least one microfluidic separation-procedure unit comprising at least one separation-procedure channel comprising (i) at least one amplification cavity configured to reproduce molecular chains supplied via the supply opening as biochemical material, and (ii) at least one separation unit microfluidically connectable or connected to the at least one amplification cavity and including a multipore material, the at least one separation unit configured to separate nucleic acids from further macromolecular constituents and/or to resolve nucleic acids; and a discharge opening configured for discharging nucleic acids resolved in the at least one separation-procedure unit from the sequencing device as biochemical material.

2 . The sequencing device as claimed in claim 1 , wherein the at least one separation unit comprises at least one of the following: porous silicon, polycarbonate filter membranes, fabric-type polymer membranes, nanoporous metal oxide, an array of microposts, an acrylamide gel, and an agarose gel.

3 . The sequencing device as claimed in claim 1 , wherein the at least one separation unit comprises at least two multipore layers of different porosity.

4 . The sequencing device as claimed in claim 1 , wherein the at least one separation unit has a width which substantially corresponds to a width of the at least one amplification cavity.

5 . The sequencing device as claimed in claim 1 , wherein the at least one amplification cavity is connectable or connected to the at least one separation unit by a wall having a bottleneck.

6 . The sequencing device as claimed in claim 1 , wherein the supply opening and/or the discharge opening is formed as a constriction of the at least one separation-procedure channel.

7 . The sequencing device as claimed in claim 1 , further comprising:
a field-development unit configured to generate an electric field across the at least one separation unit in order to convey a molecular chain through the at least one separation unit by electrophoresis.

8 . The sequencing device as claimed in claim 1 , wherein:
at least one reaction component and at least one artificial DNA nucleotide and/or one chain-termination nucleotide is kept in reserve in the at least one amplification cavity; and/or the at least one amplification cavity is configured to accommodate a liquid for an amplification reaction.

9 . The sequencing device as claimed in claim 1 , wherein:
the at least one amplification cavity of the at least one separation-procedure unit comprises at least two amplification cavities, and
each of the at least two amplification cavities contains (a) at least one identical reaction component and (b) at least one different, artificial DNA nucleotide and/or at least one respectively different chain-termination nucleotide; and/or
each of the at least two amplification cavities contains is designed to accommodate a liquid for an amplification reaction.

10 . The sequencing device as claimed in claim 1 , further comprising:
a length-determination unit configured to determine a length of resolved nucleic acids as biochemical material.

11 . A method for separating molecular chains obtained from an amplification reaction, using a microfluidic sequencing device that has (a) a supply opening configured for supplying biochemical material into the sequencing device; (b) at least one microfluidic separation-procedure unit including at least one separation-procedure channel that includes (i) at least one amplification cavity configured to reproduce molecular chains supplied via the supply opening as biochemical material, and (ii) at least one separation unit microfluidically connectable or connected to the at least one amplification cavity and including a multipore material, the at least one separation unit configured to separate nucleic acids from further macromolecular constituents and/or to resolve nucleic acids; and a discharge opening configured for discharging nucleic acids resolved in the at least one separation-procedure unit from the sequencing device as biochemical material, the method comprising:
introducing a liquid for the amplification reaction into at least one amplification cavity of at least one separation-procedure channel of at least one microfluidic separation-procedure unit in order to prepare for an amplification reaction; conducting a sealing liquid across the supply opening and the discharge opening in order to seal the supply opening and the discharge opening; performing the amplification reaction in order to generate at least one molecular chain and terminating obtained molecular chains in order to generate chain-termination products; and applying a voltage between the supply opening and the discharge opening in order to fill the at least one separation unit and separating the at least one molecular chain generated in the separation unit in order to obtain chain-termination products;

12 . The method as claimed in claim 11 , further comprising:
measuring a length of the chain-termination products in order to detect a sequence of the chain-termination products.

13 . A method for producing the sequencing device as claimed in claim 1 , the method comprising:
forming the at least one separation unit of the multipore material; and connecting the at least one separation unit to the at least one amplification cavity in the at least one separation-procedure unit in order to produce the sequencing device.

14 . A device which is configured to execute and/or control the steps of the method as claimed in claim 11 in corresponding units.

15 . A computer program which is configured to execute and/or control a microfluidic sequencing device, which has (a) a supply opening configured for supplying biochemical material into the sequencing device; (b) at least one microfluidic separation-procedure unit including at least one separation-procedure channel that includes (i) at least one amplification cavity configured to reproduce molecular chains supplied via the supply opening as biochemical material, and (ii) at least one separation unit microfluidically connectable or connected to the at least one amplification cavity and including a multipore material, the at least one separation unit configured to separate nucleic acids from further macromolecular constituents and/or to resolve nucleic acids; and a discharge opening configured for discharging nucleic acids resolved in the at least one separation-procedure unit from the sequencing device as biochemical material, so as to execute program instructions stored in memory to operate the microfluidic sequencing device to separate molecular chains obtained from an amplification reaction by:
introducing a liquid for an amplification reaction into at least one amplification cavity of at least one separation-procedure channel of at least one microfluidic separation-procedure unit in order to prepare for an amplification reaction; conducting a sealing liquid across the supply opening and the discharge opening in order to seal the supply opening and the discharge opening; performing an amplification reaction in order to generate at least one molecular chain and terminating obtained molecular chains in order to generate chain-termination products; and applying a voltage between the supply opening and the discharge opening in order to fill the at least one se

Assignees

• Bosch Gmbh Robert

Inventors

• Hoffmann Jochen

Classifications

• B01L3/50857 Primary

  using arrays or bundles of open capillaries for holding samples · CPC title

• C12Q1/6869

  Methods for sequencing · CPC title

• B01L3/50273

  characterised by the means or forces applied to move the fluids · CPC title

• B01L2300/0681

  Filter · CPC title

• B01L3/502753 Primary

  characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation · CPC title