Composition and method for preserving or culturing ocular cells

Patent metadata

Field	Value
Publication number	US-2021207088-A1
Application number	US-201917269278-A
Country	US
Kind code	A1
Filing date	Aug 30, 2019
Priority date	Aug 31, 2018
Publication date	Jul 8, 2021
Grant date	—

Abstract

The present disclosure provides a composition and method for preserving ocular cells. More specifically, the present disclosure provides a composition for preserving ocular cells, or culturing the cells after preservation, comprising albumin and dimethyl sulfoxide, and a cell formulation comprising the ocular cells, albumin, and dimethyl sulfoxide, and a treatment/prevention method using the same. The present disclosure also provides a method of preserving corneal endothelial cells, comprising suspending ocular cells in the composition to provide a suspension and freezing the suspension. In some embodiments, ocular cells are corneal cells (e.g., corneal endothelial cells).

First claim

1 .- 30 . (canceled)

31 . A method for preserving ocular cells, comprising:
suspending the ocular cells in a composition comprising albumin and dimethyl sulfoxide to provide a suspension; and freezing the suspension.

32 . The method of claim 31 , wherein the ocular cells are suspended in the suspension in a concentration of about 1×10 3 to about 1×10 8 cells/mL.

33 . The method of claim 31 , wherein the freezing the suspension comprises freezing without pre-freezing.

34 . The method of claim 33 , wherein the freezing without pre-freezing comprises freezing rapidly at about −80° C.

35 .- 70 . (canceled)

71 . The method of claim 31 , wherein the ocular cells comprise corneal cells.

72 . The method of claim 31 , wherein the ocular cells comprise corneal endothelial cells.

73 . The method of claim 31 , wherein the composition further comprises a saccharide.

74 . The method of claim 31 , wherein the composition further comprises a phosphate ion.

75 . The method of claim 31 , wherein the composition further comprises a metal ion.

76 . The method of claim 75 , wherein the metal ion is selected from the group consisting of a calcium ion, a magnesium ion, an iron ion, a zinc ion, a copper ion, an aluminum ion, and any combination thereof.

77 . The method of claim 31 , wherein the composition further comprises (i) a calcium ion and magnesium or (ii) calcium, magnesium, and copper.

78 . The method of claim 31 , wherein the albumin has a concentration of about 0.01 to about 25% (w/w) in the composition.

79 . The method of claim 31 , wherein the dimethyl sulfoxide has a concentration of about 0.1 to about 50% (w/w) in the composition.

80 . The method of claim 75 , wherein the metal ion has a concentration of about 0.0000001 to 5% (w/w) in the composition.

81 . The method of claim 31 , wherein the composition further comprises (i) about 0.1% (w/w) calcium and magnesium in the composition or (ii) about 0.1 to about 5% (w/w) calcium, magnesium, and copper in the composition.

82 . The method of claim 73 , wherein the saccharide has a concentration of about 0.1 to about 10% (w/w) in the composition.

83 . The method of claim 31 , wherein the composition is Bambanker®.

84 . The method of claim 31 , wherein the ocular cells comprise corneal endothelial cells, and the method further comprises:
preserving the suspension, and administering the preserved suspension to a subject.

85 . The method of claim 84 , wherein the preserving comprises cryopreserving the suspension.

86 . The method of claim 84 , wherein the corneal endothelial cells are not cultured prior to administering to the subject.

Assignees

• The Doshisha

Inventors

• Koizumi Noriko
• Okumura Naoki

Classifications

• A01N1/126

  Physiologically active agents, e.g. antioxidants or nutrients · CPC title

• A61K35/30

  Nerves; Brain; Eyes; Corneal cells; Cerebrospinal fluid; Neuronal stem cells; Neuronal precursor cells; Glial cells; Oligodendrocytes; Schwann cells; Astroglia; Astrocytes; Choroid plexus; Spinal cord tissue · CPC title

• C12N2500/34

  Sugars · CPC title

• C12N5/0621 Primary

  Eye cells, e.g. cornea, iris pigmented cells (photoreceptors C12N5/062) · CPC title

• C12N2500/14

  Calcium; Ca chelators; Calcitonin · CPC title