Cementation methods
US-11274323-B2 · Mar 15, 2022 · US
US2021189238A1 · US · A1
| Field | Value |
|---|---|
| Publication number | US-2021189238-A1 |
| Application number | US-202117192337-A |
| Country | US |
| Kind code | A1 |
| Filing date | Mar 4, 2021 |
| Priority date | Sep 10, 2018 |
| Publication date | Jun 24, 2021 |
| Grant date | — |
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The present disclosure provides a method of biocementation comprising contacting a granular, cohesionless soil with a solution, wherein the solution comprises urea, urease, a source of calcium ions, and a source of non-urease proteins, wherein the urea, urease, source of calcium ions, and source of non-urease proteins are provided in effective amounts suitable to cause crystallization of calcium carbonate.
Opening claim text (preview).
What is claimed is: 1 . A method of biocementation comprising: contacting a granular, cohesionless soil with a solution comprising: urea; urease; a source of calcium ions; and a source of non-urease proteins, wherein the urea, urease, source of calcium ions, and source of non-urease proteins are provided in effective amounts suitable to cause crystallization of calcium carbonate. 2 . The method of claim 1 , wherein the source of non-urease proteins comprises an enzyme stabilizer. 3 . The method of claim 2 , wherein the enzyme stabilizer comprises nonfat milk powder. 4 . The method of claim 3 , wherein the solution comprises about 4 grams/liter non-fat milk powder. 5 . The method of claim 4 , wherein the source of calcium ions comprises calcium chloride dehydrate. 6 . The method of claim 4 , wherein the solution comprises about 0.25 M to about 0.67 M calcium chloride. 7 . The method of claim 4 , wherein the solution comprises about 0.37 M to about 1 M urea. 8 . The method of claim 4 , wherein the solution comprises about 0.85 g/L to about 3 g/L urease. 9 . The method of claim 1 , wherein the enzyme stabilizer comprises casein. 10 . A method of producing an EICP-treated soil, the method comprising: contacting a granular, cohesionless soil with a solution comprising: urea; urease; a source of calcium ions; and a source of non-urease proteins, wherein the EICP-treated soil comprises a carbonate content of less than about 3% weight by weight. 11 . The method of claim 10 , wherein the EICP-treated soil comprises a carbonate content of between about 3% weight by weight and about 0.5% weight by weight. 12 . The method of claim 10 , wherein the EICP-treated soil comprises an unconfined compressive strength of about 0.5 MPa or more. 13 . The method of claim 12 , wherein the EICP-treated soil comprises an unconfined compressive strength of between about 0.5 MPa and about 2 MPa. 14 . The method of claim 12 , wherein the source of non-urease proteins comprises an enzyme stabilizer. 15 . The method of claim 14 , wherein the enzyme stabilizer comprises nonfat milk powder. 16 . The method of claim 15 , wherein the solution comprises 4 grams/liter non-fat milk powder. 17 . The method of claim 15 , wherein the solution comprises about 0.25 M to about 0.67 M calcium chloride. 18 . The method of claim 15 , wherein the solution comprises about 0.37 M to about 1 M urea. 19 . The method of claim 15 , wherein the solution comprises about 0.85 g/L to about 3 g/L urease. 20 . The method of claim 14 , wherein the enzyme stabilizer comprises casein.
containing hybrid binders other than those of the polycarboxylate type · CPC title
the additive being an organic compound · CPC title
Urease (3.5.1.5) · CPC title
Living organisms, e.g. microorganisms, or enzymes · CPC title
Consolidating by placing solidifying or pore-filling substances in the soil (making piles E02D5/46; soil-conditioning or soil-stabilising materials C09K17/00; consolidation of ground around boreholes or wells E21B43/025) · CPC title
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