Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars

Patent metadata

Field	Value
Publication number	US-2021180098-A1
Application number	US-202117170169-A
Country	US
Kind code	A1
Filing date	Feb 8, 2021
Priority date	Apr 30, 2014
Publication date	Jun 17, 2021
Grant date	—

Abstract

The invention relates to a process for the preparation of a fermentation product from lignocellulosic material, comprising the following steps:a) optionally, pretreatment of the lignocellulosic material,b) optionally, washing of the optionally pretreated lignocellulosic material,c) enzymatic hydrolysis of the optionally washed and/or optionally pretreated lignocellulosic material using an enzyme composition comprising at least two cellulases and whereby the enzyme composition at least comprises LPMO, and optionally purifying the hydrolysed lignocellulosic material,d) fermentation of the hydrolysed lignocellulosic material to produce a fermentation product, ande) optionally, recovery of a fermentation product,wherein the amounts of formed hydrolysed oxidation products at the end of the enzymatic hydrolysis by the oxidation by LPMO of the lignocellulosic material containing cellulose and/or cello-oligosaccharides is kept between 3 to 80 g/kg glucan present in the lignocellulosic material by adding a suitable amount of oxygen after the pre-treatment and before and/or during the enzymatic hydrolysis to the lignocellulosic material, preferably the formed hydrolysed oxidation product is gluconic acid, an aldonic acid and/or geminal diol, more preferably the hydrolysed oxidation product is gluconic acid.

First claim

1 . A process for the preparation of a sugar product from lignocellulosic material, comprising:
a) optionally, pretreatment of the lignocellulosic material, b) optionally, washing of the optionally pretreated lignocellulosic material, c) enzymatic hydrolysis of the optionally washed and/or optionally pretreated lignocellulosic material using an enzyme composition comprising at least two cellulases and whereby the enzyme composition at least comprises a lytic polysaccharide monooxygenase (LPMO), and d) optionally, recovery of a glucose-containing composition,
wherein the amount of formed gluconic acid at the end of the enzymatic hydrolysis by the oxidation by LPMO of the lignocellulosic material containing cellulose and/or cello-oligosaccharides is kept between 3 to 80 g/kg glucan present in the lignocellulosic material by adding a suitable amount of oxygen after the pretreatment and before and/or during the enzymatic hydrolysis to the lignocellulosic material.

2 . A process for the preparation of a fermentation product from lignocellulosic material, comprising:
a) optionally, pre-treatment of the lignocellulosic material, b) optionally, washing of the optionally pretreated lignocellulosic material, c) enzymatic hydrolysis of the optionally washed and/or optionally pretreated lignocellulosic material using an enzyme composition comprising at least two cellulases and whereby the enzyme composition at least comprises a lytic polysaccharide monooxygenase (LPMO), and optionally purifying the hydrolysed lignocellulosic material, d) fermentation of the hydrolysed lignocellulosic material to produce a fermentation product, and e) optionally, recovery of a fermentation product,
wherein the amount of formed gluconic acid at the end of the enzymatic hydrolysis by the oxidation by LPMO of the lignocellulosic material containing cellulose and/or cello-oligosaccharides is kept between 3 to 80 g/kg glucan present in the lignocellulosic material by adding a suitable amount of oxygen after the pretreatment and before and/or during the enzymatic hydrolysis to the lignocellulosic material.

3 . The process according to claim 2 , wherein the fermentation is conducted with a microorganism that is able to ferment at least one C5 sugar.

4 . The process according to claim 3 , wherein the microorganism is of the species Saccharomyces cerevisiae , in which genetic modifications have been made.

5 . The process according to claim 1 , wherein during the enzymatic hydrolysis oxygen is added to the lignocellulosic material.

6 . The process according to claim 1 , wherein the oxygen is added in the form of bubbles.

7 . The process according to claim 1 , wherein the reactor for the enzymatic hydrolysis has a volume of 1 m 3 or more.

8 . The process according to claim 1 , wherein the enzymatic hydrolysis time is 5 to 150 hours.

9 . The process according to claim 1 , wherein the enzyme composition used retains activity for 30 hours or more.

10 . The process according to claim 1 , wherein the hydrolysis is conducted at a temperature of 45° C. or more, optionally 50° C. or more and optionally at a temperature of 55° C. or more.

11 . The process according to claim 1 , wherein the enzyme composition is derived from a fungus, preferably a microorganism of the genus Rasamsonia or the enzyme composition comprises a fungal enzyme, optionally a Rasamsonia enzyme.

12 . The process according to claim 1 , wherein the dry matter content in the enzymatic hydrolysis is 10 wt % or more, optionally is 14 wt % or more and optionally is 14 to 33 wt %.

13 . The process according to claim 1 , in which the enzymatic hydrolysis takes place in a batch, fed batch and/or continuous culture reactor.

14 . The process according to claim 1 , wherein the enzyme composition is in the form of a whole fermentation broth of a fungus.

15 . The process according to claim 1 , in which oxygen is introduced as an oxygen-containing gas such as air.

Assignees

• Dsm Ip Assets Bv

Inventors

• Noordam Bertus
• Bevers Loes Elizabeth
• Parton Rudy Francois Mariajozef
• Berkhout Michael Petrus Jozef

Classifications

• C12P7/16

  Butanols · CPC title

• C12P19/02 Primary

  Monosaccharides (2-ketogulonic acid C12P7/60) · CPC title

• C12P19/14

  produced by the action of a carbohydrase {(EC 3.2.x)}, e.g. by alpha-amylase {, e.g. by cellulase, hemicellulase} · CPC title

• C12P2203/00

  Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source · CPC title

• C13K13/00

  Sugars not otherwise provided for in this class · CPC title